Alen J Salerian*
Modern Psychiatry, Athens, Greece
*Corresponding Author: Alen J Salerian, Modern Psychiatry, Athens, Greece.
Received:April 07, 2021; Published: : April 30, 2021
This paperproposes that there maybe five independent pathways for infections to develop; Transformation from organic matter, a non-living protein multiplying in a cell, evolution of microbes, reproduction-contamination and endogenous production. It has been demonstrated that the Christensenellaceae, a family in the phylum Firmicutes, is heritable suggesting that human cells and gut bacterial material are related.It has also been shown that the fetus is exposed to bacteria prior to birth -without any evidence that they are contaminants or acquired from the environment suggesting a possible endogenous origin of bacteria in breast milk, meconium, placenta, umbilical cord blood and amniotic fluid. Malassezia yeasts are not contagious, not culturable from the environment, cannot colonize human skin by inoculation without occlusion and neonate skin is free of Malassezia but is colonized in the first month of lifesuggesting that they may be endogenous. Human stem cells seem to be the most likely candidates to produce microbes: This is becausethey differentiate to epithelial cells and cancer cells and contain the essentials to transform to microorganisms. Evidence to suggest decomposer microbes and Pseudomonas aeruginosa may represent examples of microevolution of microorganisms is presented. Future experimental studies to demonstrate the transformation of organic matter to microorganisms and also the microevolution of organisms consistent with the Darwinian theory are described.
Keywords: Independent Pathways; Infections; Transformation; Organic Matter
Since the introduction of the germ theory, it has been accepted that all infections result from foreign invading microbes .
Of interest,the history of earth[2,3] and thetheory of evolutionsuggest there may be pathwaysindependent of contamination for microorganisms to develop such as transformation from lifeless organic matter or evolution of microorganisms to more complex microorganisms. It has already been established that viral infections  and prion caused diseases  result from lifeless proteins multiplying in cells of complex multi- cellular organisms. In essence, our current paradigm of all infections resulting from contamination seems to be less than accurate.
This paper reviews possible diverse origins of infections based upon scientific observations for our better understanding of diverse pathways of infections maybe helpful to combat infectious disorders. Possible diverse origins of infections will be discussed under five headings:
The history of life on earth suggests, the first microorganisms were born from lifeless organic matter some 3.6 billion years ago[2,3]. Although there is some controversy over the exact timing and the precise identity of the first living things on earth, in general, the scientific consensus indicates living organisms came from nonliving organic matter. It seems reasonable to assume that the very processes that started life 3.6 billion years ago continue to be operational now.A lifeless protein and a cell of a multicellular organism
Yet not fully understood processes involved in the formation of unicellular organisms from lifeless organicmatter may also be observed in the emergence of infectious pathogens such as virusesand prion associated disorders whereby a lifeless protein -virion or prion-gains the capacity to multiply and reproduce upon entry into a cell of a multicellular organism. Viral infections and prion caused neurodegenerative disorders representinfections that develop by various protein particles becoming pathogens in human and animal cells.Reproduction-contamination
Bacteria reproduce by cell division through binary fission. The germ theory experimentally validated by Louis Pasteur demonstrated that some infections result from bacterial growth. Indeed, the germ theory has been the central paradigm of medicine ever since its introduction in the middle of 19thcentury.Possible changes of stem cells to microorganisms
There seems to be some indirect evidence to suggest the possibility that stem cells which are multi potent and capable to differentiate to epithelial cells and cancer may also transform to microorganisms. Although this hypotheses has not been validatedby experimental studies, its potential validity is supported by diverse observations that indeed humans and some microorganismsshare genetic links.For instance, it has been demonstrated that the Christensenellaceae, a family in the phylum Firmicutes, is heritable suggesting that human genetic material and gut bacterial material are related andhuman cells may generate some gut microbes.Also, It has been shown that Malassezia yeasts are not contagious[8,9], not culturable from the environment, cannot colonize human skin by inoculation without occlusion[11,12] and neonate skin is free of Malassezia but is colonized in the first month of life  suggesting that they may be endogenous.
Furthermore, It has also been shown that the fetus is exposed to bacteria prior to birth -without any evidence that they are contaminants or acquired from the environment suggesting a possible endogenous origin of bacteria in placenta, amniotic fluid meconium breast milk,breast tissueumbilical cord blood.
In essence a plethora of observations are consistent with the possibility thatthe Christensenellaceae, Malassezia yeasts andbacteria in breast milk, meconium, placenta, umbilical cord blood and amniotic fluidare endogenous.
The hypotheses of endogenous infections may suggest thathuman stem cells are the most likely candidates to produce microbes: This is because human stem cells differentiate to epithelial cells, erythrocytes, lactocytes, cancer cells and have all the essentials to produce bacteria[22,23]. Also, the rate of gut epithelial cell production and cell loss is the same as endogenous bacterial production suggesting that fallen epithelial cells may become bacteria.Understandably,the endogenous infections hypotheses needs future experimental validation.Microevolution of microbes
Approximately 38 trillion microbesrepresent the normal flora and are still active when a mammal dies .Decomposer microbes including Pseudomonas aeruginosa play an important role in the process of mammalian decomposition [26-28]. In the early stages of decomposition, decomposer bacteria breakdown tissues and produce hydrogen sulfide methane putrescine, cadaverine, which inflate cadaverand eventually trigger the rupture of skin [26-28].The pre-bloat stage is predominated by Pseudomonas,Streptococcus, Staphylococcus, Enterobacteriacae,Prevotella,Veillonellaand Actinobacteria.
Decomposition follows a predicted clock like sequence consistent with a shift from aerobic to anaerobic and ammonium intolerant to ammonium tolerant microbes. suggesting that bacterial communities evolve andgain abilities to survive in rapidly changing environments corresponding with the stages of decomposition [26-28]. Predicted increases in genes related to nitrogen cycling and amino acid degradation including those required for the breakdown of lysine and arginine into cadaverine and putrescine have been noted [26-28]. Later, the decomposer microbial community seems to be capable to survive in ammonium rich environments [26-28].
Hyde.,et al.suggested that thedecomposer bacterial community migrated out of the large intestine, skin or soil [26,27].Metcalf at al. hypothesized that the microbial decomposersmainly come from the rich microbial biodiversity in soils .
Of interest, several observations argue against the hypotheses that soil bacteria are the predominant source of decomposers. For instance, there are some estimated 38 trillion microorganisms that reside in human body are still alive and active at the time of death .
Decomposition has been observed in pig carcasses in tightly wrapped plastic bags in water .It has also been demonstrated that in sterile soil decomposition occurred . In summary, host specific intrinsic biological processes -independent of the environment -shape the bacterial communities involved in mammalian decomposition. This observation suggests that the most likely candidate of origin of decomposer microbes including Pseudomonas are the endogenous bacteria of the host body and not contaminants from the soil. Worthy of emphasis is the observation that during mammalian decomposition bacterial communities continue to change and evolve in a predicted schedule without any evidence that those changes correspond to input from microorganisms that are getting acquired from the environment.This observation also suggests that environmental conditions shared by a dead host may promote evolution of decomposing microbes including Pseudomonas from host microbes.Pseudomonas has larger genome size and greater cellular and functional complexity-more evolved- than common bacteria.
The possible evolution of complex unicellular organisms such as pseudomonas aeruginosa from less complex unicellular organisms such as human bacterial flora lacks experimental validation however is supported by indirect scientific observations on burn wound infections and studies of decomposer species.
During mammalian decomposition host gut bacteria-and not contaminants from the environment- seem to be the predominant source of decomposers suggesting that decomposer bacteria may be endogenous and represent evolution of host bacteria. In essence, decomposer bacteria including Pseudomonas may result from contamination or may evolve from host gut bacteriaconsistent with what Charles Darwin once observed - all species have two possible pathways of origins, reproduction or evolution from a lesscomplex organism.
Scientific data suggest there may indeed multiple pathways of origin for infections to develop independent of contamination. It is true that amongmany possible pathways of infections only the germ theory has been experimentally validated.
However compelling the supporting evidence, neither the birth of first microorganisms from organic matter nor the evolution of species has been experimentally demonstrated.
Bacteria in the fetus that are not contaminants, the observation that some gutbacteria and Malassezia species are heritable strongly support an endogenous origin of some infections.
If indeed some infections are endogenous and do not result from contamination,human stem cells are the most likely candidates to produce microbes: This is because human stem cells differentiate to epithelial cells, erythrocytes, lactocytes, cancer cells and have all the essentials to produce bacteria. Also, the rate of gut epithelial cell production and cell loss is the same as endogenous bacterial production suggesting that fallen epithelial cells may become bacteria
Also of significance, the emergence of more complex microorganisms during mammalian decomposition without any evidence of their foreign origin maybe viewed as evidence of microevolution of normal gut bacteria.
It seems possible to conduct novel studies to demonstrate the transformation of organic matter to microorganisms and also the microevolution of organisms. It can easily be demonstrated that sterilized organic matter [milk and eggs] would generate microorganisms in sterile conditions in ambient temperatures. For the record, this author conducted a home experiment in which microwave sterilized eggs in sterile conditions and in temperaturesbetween 20 and 30 Celcius showed visually observable evidence of spoilage.It seems equally feasible to demonstrate microevolution of common bacteria to more complexbacteria in conditions similar to mammalian decomposition.
Further experimental validation of the central paradigm of this paper-possible multiple pathways of infections-may help usbetter combat infectious disorders.
Origin of Microbes
Origin of Microorganisms
Transformation of organic matter
Organic matter multiplying in a cellof an organism (virus,prion disease)
Transformation of cells of multicellularorganisms to microorganisms
Evolution of less complex microorganismsto more complex microorganisms.
Some gut microbes may be endogenous
· Meconium is not sterile and there is no evidence that bacteria in meconium areacquired from the environment.
· Human genetics shape gut bacteria and some gut microbes are heritable suggesting a genetic link between human cells and gut microbes.
· Human stem cells differentiate to epithelial cells, erythrocytes, lactocytes, cancer cells and have all the essentials to produce bacteria.
· The rate of gut epithelial cell production and cell loss is the same as endogenous bacterial production suggesting that fallen epithelial cells may become bacteria.
Pseudomonas may evolve from gut bacteria in a stressed host
[A stressed host -due to abnormal genetics or biology- is a prerequisite for Pseudomonas aeruginosa infections]
· Healthy hosts do not develop pseudomonas infections.
· Healthy volunteers did not develop infection after ingesting Pseudomonas.
· Normal mice exposed to pseudomonas do not develop infection .
· Injury is associated with Pseudomonas infection.
· Cerebrovascular accident is associated with Pseudomonas infection.
· Burn wound is associated with Pseudomonas infection[6-8].
· Heritable Cystic fibrosis is associated with Pseudomonas infection[9-13].
· Heritable Crohn’s disease is associated with Pseudomonas infection[14-18].
· Decomposition of corpses is associated with emergence of decomposers including Pseudomonas not acquired from the environment[19-23].
· Pseudomonas has larger genome size,greater cellular and functional complexity-more evolved- than common bacteria.
Citation: : Alen J Salerian., et al. “Origin of Infections: Five Independent Pathways". Acta Scientific Pharmaceutical Science 5.5 (2021): 88-92.
Copyright: © 2021 :Alen J Salerian., et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.