Acta Scientific Pharmaceutical Sciences (ASPS)(ISSN: 2581-5423)

Research Article Volume 5 Issue 1

Partial Purification of Superoxide Dismutase and Antigenicity Nature in Setaria digitata, A Filarial Parasite

Jayakumar K1*, Dhanya NV2 and Sony Peter3

1Professor and Head, Department of Biochemistry, PM S College of Dental Science and Research, Vettapara, Thiruvanthapuram, Kerala, India
2Reader, Department of Biochemistry, Sri Sankara Dental College, Varkala Thiruvanthapuram, Kerala, India
3Senior Lecturer, Department of Biochemistry, PM S College of Dental Science and Research, Vettapara, Thiruvanthapuram, Kerala, India

*Corresponding Author: Jayakumar K, Professor and Head, Department of Biochemistry, PM S College of Dental Science and Research, Vettapara, Thiruvanthapuram, Kerala, India.

Received: November 17, 2020; Published: December 10, 2020

×

Abstract

  Setaria digitata is a filarial worm of the cattle used as a model system for anti- filarial drug screening, due to its similarity to the human filarial parasites Wuchereria bancrofti and Brugia malayi. Since filarial Superoxide dismutase (SOD) is a good biochemical target for anti filarial drug development, a study has been undertaken for the biochemical characterization of SOD from S. digitata. Superoxide dismutase activity was measured in different stages of growth of filarial parasites (human and cattle). The activity was almost undetected or very low in micro filarial stage but in adult worms, the enzyme activity was high. The enzyme was characterized to be a Cu/Zn superoxide dismutase. Most of the enzyme activity was associated with a detergent extractable fraction of adult (Setaria) parasite. The enzyme was also detected in the in vitro released products of adult worms. The superoxide dismutase activity was completely inhibited with IgG antibody from chronic filarial patients in contrast to IgG from normal people. Filarial patients particularly have high IgG antibody levels to purified enzyme. However, individuals from non-filarial regions of Trivandrum, Kerala, sero-negative for superoxide dismutase antibodies. Antibody response to superoxide dismutase could thus be used for filarial diagnosis.

Keywords: Filariasis; Superoxide Dismutase; Wuchereria bancrofti; Setaria digitata

×

References

  1. Lamas J., et al. “Resveratrol promotes an inhibitory effect on the turbot Scutico ciliate parasite Philasteridesdicentrarchi by mechanisms related to cellular detoxifcation”. Vertinary Parasitology 161 (2009): 307-315.
  2. Sattin G., et al. “Characterization and expression of a new cytoplasmic glutathione peroxidase 1 gene in the Antarctic fish Trematomus bernacchii”. Hydrobiologia 761 (2015): 363-372.
  3. Ferro K., et al. “Cu, Zn SOD genes in Tribolium castaneum: evolution, molecular characterisation and gene expression during immune priming”. Frontier Immunology 8 (2017): 1811-1821.
  4. Ferro D., et al. “Molecular characterization and metal induced gene expression of the novel glutathione peroxidase 7 from the chordate invertebrate Cionarobusta”. Compartive Biochememistry and Physiology C 205 (1018) : 1-7.
  5. Kim J S., et al. “Identification and molecular characterization of two Cu/Zn-SODs and Mn-SOD in the marine ciliate Euplotes crassus: Modulation of enzyme activity and transcrips in response to copper and cadmium”. Aquatic Toxicology 199 (2018): 296-304.
  6. Gieseg S., et al. “Peroxidation of proteins before lipids in U937 cells exposed to peroxyl radicals”. Biochemistry Journal 350 (2000): 215-218.
  7. Callahan Η L., et al. “Helminth antioxidant enzymes: A protective mechanism against host oxidants” Parasitolgy Today 4 (1988): 218-223.
  8. Hong Z., et al. “Identification and purification of a second form of Cu/Zn Superoxide dismutase from Schistosoma mansoni”. Infection and Immunology 60 (1992): 3641-3651.
  9. Rhoads ΜL., et al. “Identification and purification of superoxide dismutase”. Experimental Parasitology 56 (1983): 41-54.
  10. Leid R W and Suquet C Μ “A Superoxide dismutase of meta cestodes of Taeniataeniae formis”. Molecular Biochemical Parasitology 18 (1986): 301-311.
  11. Fairfield A S., et al. “Superoxide dismutase and catalase in the murine malaria, Plasmodium berghei; Content and sub-cellular distribution”. Archives of Biochemistry and Biophysics 250 (1986): 526-529.
  12. Henkle Κ J., et al. “Characterization and molecular cloning of a Cu/Zn Superoxide dismutase from the human parasite Onchocerca volvulus”. Infection and Immunology 59 (1991): 2063-2069.
  13. Sugunan VS and Kaleysa Raj R. “Orgin of Biologically active excretory secretory materials from Setaria digitata”. Current Science 55 (1986): 331-533.
  14. Paoletti F and Macali A “ Determination of Superoxide dismutase activity by purely chemical system based on NAD (P)H oxidation”. Methods in Enzymology 186 (1990): 209-220.
  15. Nishikimi M., et al. “The occurrence of superoxide dismutase (SOD’s) in controlling oxidative stress in plants”. Biochemical Biophysical Research Communications 46 (1972): 844-845.
  16. Das Μ Κ., et al. “Immunoglobulin Ε and G4 antibodies to infective larvae in a Wuchereria bancrofti endemic population”. International Archives of Allergy and Immunology 99 (1992): 118-122.
  17. Bradford MM. “A rapid and sensitive method for the quantization of microgram quantities of protein utilizing the principle of protein-dye binding”. Annals of Biochemistry 729 (1976): 248-254.
  18. Newport G., et al. “Parasite heat shock proteins”. Parasitology Today 11 (1988): 306-312.
  19. Laemmli UK. “Cleavage of structural proteins during the assembly of the head of bacteriophage T4”. Nature (Lond) 227 (1970) 680-685.
  20. James E R., et al. “Molecular cloning of an Onchocer cavolvulus extracellular Cu-Zn superoxide dismutase”. Infection and Immunology 62 (1994) :713-716.
  21. Kim TS., et al. “Molecular Cloning and Expression of Cu/Zn-Containing Superoxide dismutase from Fasciola hepatica”. Infection and Immunity7 (2000): 3941-3948.
  22. Paoletti F and Macali A “Determination of Superoxide dismutase activity by purely chemical system based on NAD (P)H oxidation”. Methods in Enzymology 186 (1990) 209-220.
  23. Fridovich I. Annual review of Biochemistry 44 (1978) :178.
  24. Marklund S L. “Role of superoxide dismutase in controlling oxidative stress in parasites”. Federation of European Biochemistry Society Letters 184 (1988): 234.
  25. Chandler A C. “Studies on the nature of immunity to intestinal helminths -The locai nature of the immunity of white rats to Nippo strongylus infection”. American Journal of Hygiene 22 (1935): 157-168.
  26. Britton C., et al. “The secreted and somatic proteinases of the bovine lungworm Dictyocaulus viviparous and their inhibition by antibody from infected and vaccinated animals”. Parasitology 105 (1992): :325-333.
  27. Knox D Ρ and Kennedy Μ W. “Proteinases released by the parasitic larval stages of Ascarissuum and their inhibition by antibody”. Molecular Biochemical Parasitology 28 (1988): 207-216
  28. Kumar S., et al. “Selective modification and immune evasion: A hypothesis”. Immunology and Cell Biology 71 (1993): 141-143.
  29. Kennedy Μ W., et al. “Parasitic nematodes-antigens membranes and genes”. (London: Taylor and Francis) (1991).
  30. Kaushal NA., et al. “Isolation of an antigen fraction from Setaria cervi adults having potential for immunodiagnosis of human filariasis”. Immunological Investigations 38 (2009): 749-761.
  31. Beyer W., et al. “Superoxide dismutases”. Progress in Nucleic Acid Research and Molecular Biology 40 (1991): 221-253 .
  32. Kim J S., et al. “Identification and molecular characterization of two Cu/Zn-SODs and Mn-SOD in the marine ciliate Euplotes crassus: Modulation of enzyme activity and transcripts in response to copper and cadmium”. Aquatic Toxicology 199 (2018): 296-304.
×

Citation

Citation: Jayakumar K., et al. “Partial Purification of Superoxide Dismutase and Antigenicity Nature in Setaria digitata, A Filarial Parasite". Acta Scientific Pharmaceutical Sciences 5.1 (2021): 17-23.




Metrics

Acceptance rate32%
Acceptance to publication20-30 days
Impact Factor0.759

Indexed In




News and Events


  • Certification for Review
    Acta Scientific certifies the Editors/reviewers for their review done towards the assigned articles of the respective journals.
  • Submission Timeline for Upcoming Issue
    The last date for submission of articles for regular Issues is September 30, 2021.
  • Publication Certificate
    Authors will be issued a "Publication Certificate" as a mark of appreciation for publishing their work.
  • Best Article of the Issue
    The Editors will elect one Best Article after each issue release. The authors of this article will be provided with a certificate of “Best Article of the Issue”.
  • Welcoming Article Submission
    Acta Scientific delightfully welcomes active researchers for submission of articles towards the upcoming issue of respective journals.
  • Contact US