Abstract

  Setaria digitata is a filarial worm of the cattle used as a model system for anti- filarial drug screening, due to its similarity to the human filarial parasites Wuchereria bancrofti and Brugia malayi. Since filarial Superoxide dismutase (SOD) is a good biochemical target for anti filarial drug development, a study has been undertaken for the biochemical characterization of SOD from S. digitata. Superoxide dismutase activity was measured in different stages of growth of filarial parasites (human and cattle). The activity was almost undetected or very low in micro filarial stage but in adult worms, the enzyme activity was high. The enzyme was characterized to be a Cu/Zn superoxide dismutase. Most of the enzyme activity was associated with a detergent extractable fraction of adult (Setaria) parasite. The enzyme was also detected in the in vitro released products of adult worms. The superoxide dismutase activity was completely inhibited with IgG antibody from chronic filarial patients in contrast to IgG from normal people. Filarial patients particularly have high IgG antibody levels to purified enzyme. However, individuals from non-filarial regions of Trivandrum, Kerala, sero-negative for superoxide dismutase antibodies. Antibody response to superoxide dismutase could thus be used for filarial diagnosis.

Keywords: Filariasis; Superoxide Dismutase; Wuchereria bancrofti; Setaria digitata

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Citation

Citation: Jayakumar K., et al. “Partial Purification of Superoxide Dismutase and Antigenicity Nature in Setaria digitata, A Filarial Parasite". Acta Scientific Pharmaceutical Sciences 5.1 (2021): 17-23.

Copyright

Copyright: © 2021 Jayakumar K., et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.