Ayushi Rajput, Sourabh Jain* and Karunakar Shukla
College of Pharmacy, Dr. A. P. J. Abdul Kalam University, Indore, MP, India
*Corresponding Author: College of Pharmacy, Dr. A. P. J. Abdul Kalam University, Indore, MP, India
Received: September 02, 2020; Published: September 28, 2020
The plant Gmelina arborea (G. arborea) has been traditionally used in India for several medicinal purposes like anthelmintic, diuretic, antibacterial, antioxidant and antidiabetic. The aim of the present study was to evaluate qualitative phytochemical analysis, antioxidant and antiarthritic activities of aqueous extracts of G. arborea flowers. Qualitative analysis of various phytochemical constituents was determined by the well-known test protocol available in the literature. The in vitro antioxidant activity was assessed against 2, 2-diphenyl-1-picrylhydrazyl (DPPH) and superoxide scavenging assay using standard protocols. The in vitro antiarthritic potential of extracts was evaluated in terms of its inhibition of protein denaturation and membrane stabilization method. Freund’s adjuvant arthritis model in Wistar rats was used to study the in vivo antiarthritic activity. Phytochemical analysis revealed the presence of alkaloids, glycosides, phenols, flavonoids, tannins. The inhibitory concentration 50% values are 85.43 and 107.59 µg/ml for DPPH radical scavenging assay and superoxide scavenging assay, respectively. The plant extracts exhibited dose dependent inhibition of protein denaturation and also protected the RBC cells by stabilizing the membranes. G. arborea flowers extracts (100 and 200 mg/kg) was given orally to arthritic rats induced with complete freund’s adjuvant and changes in paw diameter, joint diameter, arthritic score and body weight were determined. Indomethacin was taken as standard. Rats treated with extracts displayed marked reduction in paw diameter, joint diameter and arthritic score along with substantial enhancement in body weight. The antiarthritic effects of these plant extracts may attribute either due to its inhibition of protein denaturation or stabilizing the membranes from the free radical attack which are generated due to the immunological and inflammatory reactions observed in most of the arthritic conditions.
Keywords: Gmelina arborea; Phytochemical Analysis; Antioxidant; Antiarthritic Activity
Citation: Sourabh Jain., et al. “Phytochemical Analysis, Antioxidant and Antiarthritic Activity of Aqueous Extracts of Gmelina arborea Flowers". Acta Scientific Pharmaceutical Sciences 4.10 (2020): 165-171.
Copyright: © 2020 Sourabh Jain., et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.