Abstract

A new simple, precise method was developed for Acebrophylline, Montelukast sodium and Levocetirizine by using UPLC for Simultaneous Estimation. Acebrophylline is combination of Theophylline and Ambroxol. The compounds were separated by using column C- 18 BEH 2.1 x 50 mm (1.7 µm) at ambient temperature. Flow rate was 0.6 ml/min; wavelength of 230 nm, Mobile phase was methanol:acetonitrile: Buffer (30:40:30) potassium dihydrogen phosphate buffer was used with pH 6.0. The injection volume was 2 µl and Run time 4.5 min. The retention time of Acebrophylline (Theophylline and Ambroxol), Montelukast sodium and Levocetirizine 0.87,1.34, 2.01, 3.20 mins respectively. The percentage purity was found to be 99.72, 99.96 and 99.81 respectively. The validation parameters Specificity, Accuracy, linearity, LOD, LOQ and Robustness were studied. The linearity of was Acebrophylline 40 - 320 µg/ml, for Montelukast sodium 2 - 16 µg/ml and for Levocetirizine 1-8 µg/ml They have good linearity with Correlation coefficient Value of 0.996, 0.994, 0.994 respectively.

Keywords: UPLC; Acebrophylline; Montelukast Sodium and Levocetirizine; Simultaneous Estimation

References

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Citation

Citation: S Sangeetha., et al. “Simultaneous Estimation of Acebrophylline, Montelukast Sodium and Levocetirizine by RP-UPLC Method in Combined Dosage Forms". Acta Scientific Pharmaceutical Sciences 4.10 (2020): 52-55.

Copyright

Copyright: © 2020 S Sangeetha., et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.