Abstract

Heat shock protein70 associated with lymphatic filariasis (LF) has been identified from a cattle filarial parasite Setaria digitata. A heat shock protein was detected in different life stages of Setaria digitata when exposed to an elevated temperature of 44°C. A combination of ATP-agarose column chromatography and electro-elution was used for its purification from adult female extract. On closer examination, it migrated as a single band at 62 kDa on 10% SDS-PAGE. These observations suggest a plausible biological connection of ScHSP70 with the disease and its strong immunogenic nature. ScHSP70 showed antigenic cross-reactivity with IgG class of antibody in different categories of filarial sera. However, when IgG subclasses were tested, IgG4 showed high specificity and sensitivity with symptomatic microfilaraemic sera.

Keywords: ATP-Agarose; Filarial Parasite; Heat Shock Proteins; Setaria digitata; W. bancrofti; SDS-PAGE

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Citation

Citation: Jayakumar K., et al. “Identification of Heat Shock Protein70 in Setaria digitata and its Evaluation as Diagnostic Marker for Lymphatic Filariasis". Acta Scientific Pharmaceutical Sciences 4.9 (2020): 15-22.

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Copyright: © 2020 Jayakumar K., et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.