Acta Scientific Pharmaceutical Sciences (ASPS)(ISSN: 2581-5423)

Research Article Volume 4 Issue 9

Identification of Heat Shock Protein70 in Setaria digitata and its Evaluation as Diagnostic Marker for Lymphatic Filariasis

Jayakumar K1*, Sony Peter2 and Sarath Babu K3

1Professor and Head, Department of Biochemistry, PMS College of Dental Science and Research, Vettapara, Thiruvanthapuram, Kerala, India
2Senior Lecturer, Department of Biochemistry, PMS College of Dental Science and Research, Vettapara, Thiruvanthapuram, Kerala, India
3Associate Professor, Department of Pharmacology, Dhanalakshmi Srinivasan Medical College and Hospital, Perambalur, Trichy, Tamil Nadu, India

*Corresponding Author: Jayakumar K, Professor and Head, Department of Biochemistry, PMS College of Dental Science and Research, Vettapara, Thiruvanthapuram, Kerala, India.

Received: July 21, 2020; Published: August 27, 2020

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Abstract

Heat shock protein70 associated with lymphatic filariasis (LF) has been identified from a cattle filarial parasite Setaria digitata. A heat shock protein was detected in different life stages of Setaria digitata when exposed to an elevated temperature of 44°C. A combination of ATP-agarose column chromatography and electro-elution was used for its purification from adult female extract. On closer examination, it migrated as a single band at 62 kDa on 10% SDS-PAGE. These observations suggest a plausible biological connection of ScHSP70 with the disease and its strong immunogenic nature. ScHSP70 showed antigenic cross-reactivity with IgG class of antibody in different categories of filarial sera. However, when IgG subclasses were tested, IgG4 showed high specificity and sensitivity with symptomatic microfilaraemic sera.

Keywords: ATP-Agarose; Filarial Parasite; Heat Shock Proteins; Setaria digitata; W. bancrofti; SDS-PAGE

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Citation

Citation: Jayakumar K., et al. “Identification of Heat Shock Protein70 in Setaria digitata and its Evaluation as Diagnostic Marker for Lymphatic Filariasis". Acta Scientific Pharmaceutical Sciences 4.9 (2020): 15-22.




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