Acta Scientific Pharmaceutical Sciences (ASPS)(ISSN: 2581-5423)

Case Report Volume 4 Issue 8

Extraction of Chromium(III) from Urine and Water Samples Using Divalent Beta-diketonates Ligands and Determination with Reversed-Phase HPLC

David TanyalaTakuwa*

Department of Chemistry, University of Botswana, Gaborone, Botswana

*Corresponding Author: David TanyalaTakuwa, Department of Chemistry, University of Botswana, Gaborone, Botswana.

Received: June 26, 2020; Published: July 28, 2020

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Abstract

 Chromium is an element of interest due to its physiological properties in different oxidation states. Chromium (III) is believed to be beneficial in curbing effects of sugar diabetes in the body, and at the same time, chromium (VI) is carcinogenic. A lot of research has been conducted in an effort to conduct speciation to identify the Cr oxidation state available in a sample. Several methods have been used but with challenges. In this study, acetylacetonates and its derivatives was used to extract Cr(III) in urine and fresh water samples. It was observed that smaller ligands of acetylacetonate metal chelates gave high linear range, which decreased with increase in the size of the ligand. Cr(AA)3, Cr(DPM)3 and Cr(DBM)3 gave linear range of 1 - 10000gn/ml, 1 - 1000 ng/mL and 1000 ng/mL respectively. The highest detection limit was observed when determining Cr(DBM)3, which had benzene rings (0.84 ng/mL) as compared to the smaller ligand metal complex Cr(AA)3 with 1.20 ng/mL. The use of ligand with benzene ring (DBM) further allowed for resolution of other metal complexes during the extraction of Cr(III), which avoided challenges of interferences. The method developed was applied successfully in real urine and fresh water samples using DBM as a chelating agent and obtained Cr(III) in the ranges 0.86 - 7.18 ng/mL and 5.94 - 8.99 ng/mL respectively. The percentage recovery was in the range 96 - 99% for fresh water and 99.8 - 105% for urine samples.

Keywords: Metal Chelates; Acetylacetonate;Dibenzoylmethane; Dipivaloylmethane; Chromium(III); High Performance Liquid Chromatography

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Citation

Citation: David TanyalaTakuwa. “Extraction of Chromium(III) from Urine and Water Samples Using Divalent Beta-diketonates Ligands and Determination with Reversed-Phase HPLC". Acta Scientific Pharmaceutical Sciences 4.8 (2020): 85-97.




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