Acta Scientific Pharmaceutical Sciences (ISSN: 2581-883X)

Research Article Volume 4 Issue 8

Development and Evaluation of Herbal Cream of Berberis aristate for its Antibacterial and Antifungal Activity

Megha Tiwari1, Mohd Neyaz Ahsan2, Pradeep K Sharma1, Anzarul Haque3 and Sanjar Alam1*

1Department of Pharmaceutics, RV Northland Institute, Greater Noida, India
2Department of Medical Laboratory Technology, University Polytechnic, Birla Institute of Technology, Mesra, Ranchi, India
3College of Pharmacy, Prince Sattam Bin Abdul Aziz University, Alkharj, KSA

*Corresponding Author: Sanjar Alam, Associate Professor, Department of Pharmaceutics, RV Northland Institute, Greater Noida, India.

Received: June 24, 2020; Published: July 18, 2020



In the present study, an attempt was made to formulate and evaluate herbal cream of Berberis aristate (B. aristata) for the management of Psoriasis. The study is intended to be carried out because of low cost, lesser or no side effects of herbal formulations and its potent action than the allopathic medication. They therefore provide a viable alternative for psoriasis management. The drug was evaluated on the basis of U.V, FT-IR, and DSC study. The herbal cream formulations were prepared by emulsion based method. The prepared cream was evaluated on the basis of viscosity, spreadability, grittiness, and in-vitro drug release. With altering the ratio of base different release patterns of the drug were observed. With an increase in the base ratio, there was increase in drug release of formulation (F1), while further increase in the base ratio leads to initial burst release but as the time proceeds the release of the drug got decreased due to increase in grittiness and viscosity of the formulation (F2). Also decrease in the base ratio leads to decrease in the drug release of formulation F3. The FT-IR and DSC analysis indicates that there was no interaction between drug and excipients. The in vitro drug release kinetics study depict that drug release mechanism follows Fickian diffusion. The optimized F1 formulation which shows satisfactory in vitro release was tested for anti bacterial and anti fungal property. The zone of inhibition was found to be 24.66 ± 1.14 and 20.45 ± 1.26 for bacteria and fungi respectively at 250 ppm.

Keywords: Berberis aristate ; Herbal Cream; Emulsion Based Method; Antifungal; Antibacterial Activity; Skin Irritancy Test and Release Models



  1. Tanweer A Syed., et al. “Management of psoriasis with Aloe Vera extract in a hydrophilic cream: a placebo-controlled, double-blind study”. Tropical Medicine and International Health4 (1996): 505-509.
  2. Kennet GL., et al. “Psoriasis: Current perspectives with an emphasis on treatment”. The American Journal of Medicine6 (1990): 595-605.
  3. Rakesh K Sindhu., et al. “Psoriasis and herbal care: a brief review”. International Journal of Pharma Research and Development9 (2007): 1-8.
  4. Goyal S., et al. “Novel Anti-Inflammatory Topical Herbal Gels Containing Withania somnifera and Boswellia serrata”. International journal of Pharmaceutical and Biological Archive4 (2011): 1087-1094.
  5. Chakravarti KK., et al. “Alkaloidal constituents of the bark of aristate (Rassaut)”. Journal of Scientific and Industrial Research 9B (1950): 161-164.
  6. Poornima Agrawal., et al. “Formulation and evaluation of herbal gel containing Boswellia serrata, Curcuma longa extract and oil of wintergreen for rheumatoid arthritis”. International Bulletin of Drug Research3 (2012): 31-40.
  7. Bairwa Ranjan., et al. “Standardization and phytochemical investigation of Berberis aristate”. Asian Journal of Pharmaceutical Analysis 3 (2012): 81-84.
  8. Chen QH., et al. “Determination of colchicine in mouse plasma by high performance liquid-chromatographic method with UV detection and its application to pharmacokinetic studies”. Yakugaku Zasshi 9 (2007): 1485-1490.
  9. Hemant Shigwan., et al. “HPLC Method Development and Validation for Quantification of Berberine from Berberis aristate and Berberis tinctorial”. International Journal of Applied Sciences and Engineering 2 (2013): 203-211.
  10. M Shahid., et al. “Ethnobotanical studies on berberis aristate DC Root Extracts”. African Journal of Biotechnology4 (2009): 556-563.
  11. Namrata Singh., et al. “Formulation and Evaluation of Meloxicam Microspheres Using Eudragit S-100”. IJPRS2 (2014) 330-339.
  12. Ashish Aswal., et al. “Preparation and evaluation of polyherbal cosmetic cream”. Scholars Research Library, Der Pharmacia Lettre 1 (2013): 83-88.
  13. Gidwani Beena., et al. “Evaluation of a Novel Herbal Formulation in the Treatment of Eczema with Psoralea Corylifolia”. Iranian Journal of Dermatology, Winter54 (2010): 122-127.
  14. Kumar L and Verma R. “In-vitro evaluation of topical gel prepared using natural polymer”. International Journal of Drug Delivery 2 (2010): 58-63.
  15. Pasrija Anubhuti., et al. “Comparative study on the antimicrobial activity of berberis aristate from different regions and berberine in- vitro”. International Journal of Life Science and Pharma Research1 (2011): 17-20.
  16. SA Hussain., et al. “In-vitro Antimicrobial Activity of Polyherbal Formulation”. Journal of Pharmaceutical Research and Opinion3 (2011): 94-95.
  17. S Alam., et al. “Microemulsion as a Potential Transdermal Carrier for Poorly Water Soluble Antifungal Drug Itraconazole”. Journal of Dispersion Science and Technology 31 (2010): 84-94.
  18. S Sujith Nair., et al. “Evaluation of Skin Irritation of Herbal Antioxidant Cream”. Asian Journal of Biochemical and Pharmaceutical Research3 (2012): 184-189.
  19. Arun K Mishra., et al. “Evaluation of Skin Irritation of Herbal O/W Sunscreen Cream on Rabbit Model”. IJPI’s Journal of Pharmaceutics and Cosmetology3 (2011): 45-49.
  20. BH More., et al. “Evaluation for Skin irritancy testing of developed formulations containing extract of Butea monosperma for its topical application”. International Journal of Toxicology and Applied Pharmacology1 (2013): 10-13.
  21. Aqil M., et al. “Matrix type transdermal drug delivery system of metoprolol tartrate: Skin toxicity and in-vivo characterization”. Ethiopian Pharmaceutical Journal 1 (2004): 53-60.


Citation: Sanjar Alam., et al. “Development and Evaluation of Herbal Cream of Berberis aristate for its Antibacterial and Antifungal Activity".Acta Scientific Pharmaceutical Sciences 4.8 (2020): 12-19.


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