Acta Scientific Pharmaceutical Sciences (ISSN: 2581-883X)

Research Article Volume 4 Issue 7

Development and Validation of Visible Spectrophotometric Methods for the Analysis of Etravirine: Application to Tablet Dosage Forms

Murali Dadi1*, Indra Sen Singh1 and G Purna Chandra Rao2

1Department of Chemistry, Copperbelt University, Jambo Drive, Kitwe, Zambia
2Department of Chemistry, NRI Institute of Technology, Pothavarpadu, Agiripalli, Andhra Pradesh, India

*Corresponding Author: Murali Dadi, Department of Chemistry, Copperbelt University, Jambo Drive, Kitwe, Zambia.

Received: April 17, 2020; Published: June 15, 2020

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Abstract

The present study describes the development and validation of two simple, sensitive, accurate, precise and economical spectrophotometric methods for the estimation of Etravirine (ETR) in bulk and its tablet dosage forms. These methods have developed based on the diazotization of ETR followed by coupling with alkaline β-naphthol (Method-A) and p-nitro aniline in alkaline medium (Method-B). These methods show a linear relation between absorbance and concentration of ETR in the ranges of 5 - 30 μg/mL (MA) and 2.5 - 15 μg/mL(M-B) respectively. The Molar absorptive for present methods are 3.685 x 103 (M-A) and 5.614 x 104 (M-B) and LOD, LOQ values are 0.656 μg/mL, 1.990 μg/mL (M-A) and 0.191 μg/mL, 0.578 μg/mL (M-B) respectively. The common excipients in the drug did not interfere in the estimation process and the developed methods are successfully applied to tablet dosage forms of ETR.

Keywords: Etravirine; β-Naphthol; p-Nitro Aniline; Alkaline Medium; Spectrophotometry

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Citation

Citation: Murali Dadi., et al. “Development and Validation of Visible Spectrophotometric Methods for the Analysis of Etravirine: Application to Tablet Dosage Forms".Acta Scientific Pharmaceutical Sciences 4.6 (2020): 15-19.



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