Sarita Yadav* and Sudha Yadava
Department of Chemistry, D.D.U. Gorakhpur University, Gorakhpur, U.P. India
*Corresponding Author: Sarita Yadav, Department of Chemistry, D.D.U. Gorakhpur University, Gorakhpur, U.P. India.
Received: January 11, 2020; Published: March 11, 2020
The rutin degrading enzyme was secreted by Fusarium croockwellen MTCC-2084 in liquid culture medium which was specific for releasing the disaccharide rutinose from the rutin with no release of glucose, rhamnose and isoquercetin. The conversion of rutin to quercetin was optimal at pH 5.0 and 65º C temperature in the presence of 0.5% dimethyl sulfoxide. The crude enzyme does not hydrolyzed hesperidin and naringin. On the basis of these result it was concluded that the crude enzyme from F. croockwellen MTCC -2084 is hydrolyzed rutin in to quercetin and rutinose which is rare bioactive compounds.
Keywords: Biotransformation; Rutin; Degrading Enzyme; Fusarium croockwellen ; Quercetin
Citation: Sarita Yadav and Sudha Yadava. “Rutin Degrading Enzyme from Fusarium croockwellen MTCC-2084". Acta Scientific Pharmaceutical Sciences 4.4 (2020): 23-27.
Copyright: © 2020 Sarita Yadav and Sudha Yadava. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.Copyright