Acta Scientific Pharmaceutical Sciences (ASPS)(ISSN: 2581-5423)

Research Article Volume 4 Issue 4

Rutin Degrading Enzyme from Fusarium croockwellen MTCC-2084

Sarita Yadav* and Sudha Yadava

Department of Chemistry, D.D.U. Gorakhpur University, Gorakhpur, U.P. India

*Corresponding Author: Sarita Yadav, Department of Chemistry, D.D.U. Gorakhpur University, Gorakhpur, U.P. India.

Received: January 11, 2020; Published: March 11, 2020

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Abstract

  The rutin degrading enzyme was secreted by Fusarium croockwellen MTCC-2084 in liquid culture medium which was specific for releasing the disaccharide rutinose from the rutin with no release of glucose, rhamnose and isoquercetin. The conversion of rutin to quercetin was optimal at pH 5.0 and 65º C temperature in the presence of 0.5% dimethyl sulfoxide. The crude enzyme does not hydrolyzed hesperidin and naringin. On the basis of these result it was concluded that the crude enzyme from F. croockwellen MTCC -2084 is hydrolyzed rutin in to quercetin and rutinose which is rare bioactive compounds.

Keywords: Biotransformation; Rutin; Degrading Enzyme; Fusarium croockwellen ; Quercetin

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References

  1. Kreft I., et al. “Rutin content in Buckwheat (Fagopyrum esculentum Moench) food material and products”. Food Chemistry 98 (2006): 508-512. 
  2. Fathiazad F., et al. “Extraction of Favonoids and Quantification of Rutin from waste Tobacco Leaves”.  Iranian Journal of Pharmaceutical Research 3 (2006): 222-227.
  3. Violeta N., et al. “Nutritional and bioactive compounds in dried tomato processing waste”. Journal of Food 16.1 (2018): 222-229.
  4. Iacopini P., et al. “Catechin, epicatechin, quercetin, rutin and resveratrol in red grape: Content, in vitro antioxidant activity and interactions”. Journal of Food Composition and Analysis 21 (2008): 589- 598.
  5. Gattuso G., et al. “Flavonoid Composition of Citrus Juices”. Molecules 12 (2007): 1641-1673. 
  6. Kreft S., et al. “Extraction of rutin from buckwheat (Fagopyrum esculentum Moench) seeds and determination by capillary electrophoresis”. Journal of Agricultural and Food Chemistry 47 (1999): 4649-4652.
  7. Kuntic V., et al. “Isocratic RP-HPLCmethod for rutin determination in solid oral dosage forms”. Journal of Pharmaceutical and Biomedical Analysis 43 (2007): 718-721.
  8. Daniel S., et al. “α‐L‐Rhamnosyl‐β‐D‐glucosidase (Rutinosidase) from Aspergillus niger: Characterization and Synthetic Potential of a Novel Diglycosidase”. Advanced Synthesis and amp; Catalysis 357.1 (2015): 107-117. 
  9. Yasuda T and Nakagawa H. “Purification and Characterization of rutin degrading enzymes in tartary buckwheat seeds”. Photochemistry 37 (1994): 133-136.
  10. Narikawa T., et al. “A beta-rutinosidase from Penicillium rugulosum IFO 7242 that is a peculiar flavonoid glycosidase”. Bioscience, Biotechnology, and Biochemistry 64 (2000): 1317-1319.
  11. Hendson M., et al. “Distribution among Pseudomonads of sequences homologous to the rutin glycosidase and B- glucosidase genes of Pseudomonas viridiflava”. Phytopathology 82 (1992): 1223-1233. 
  12. Suzuki T., et al. “Effect of UV radiation, cold and desiccation stress on rutin concentration and rutin glucosidase activity in tartary buckwheat (Fagopyru tataricum) leaves”. Plant Science 168 (2005): 1303-1307. 
  13. Nam HK., et al. “Quercetin production from rutin by a thermostable β-rutinosidase from Pyrococcus furiosus”. Biotechnology Letter 34 (2012): 483-489.
  14. Gargouri R., et al. “Protective effect of Quercetin against oxidative stress caused by dimethoate in human peripheral blood lymphocytes”. Lipids Health Disease 11 (2011): 149-153. 
  15. Stewart LK., et al. “Quercetin transiently increases energy expenditure but persistently decreases circulating markers of inflammation in C57BL/6J mice fed a high-fat diet”. Metabolism 57 (2008): S39-S46. 
  16. Hosel W. Glycosylation and Glycosidase. I. The Biochemistry of plant, a comprehensive treatise. secondary plant product. Ed. Conn, E.E., Academic press, New York (1981): 725-764.
  17. Westlake DWS., et al. “Microbial decomposition of rutin”. Canadian Journal of Microbiology 5 (1959): 621-629. 
  18. Suzuki H. “Hydrolysis of flavonoid glycosides by enzymes (rutinosidase) from rhamnus and other source”. Archives of Biochemistry and Biophysics 99 (1962): 476-483.
  19. Baumgertel A., et al. “Purification and Characterization of a flavonol-3-O-B-heterodisaccharide from the dried herb of Fogopyrum esculentum Monench”. Phytochemistry 64 (2003): 411-418.
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Citation

Citation: Sarita Yadav and Sudha Yadava. “Rutin Degrading Enzyme from Fusarium croockwellen MTCC-2084". Acta Scientific Pharmaceutical Sciences 4.4 (2020): 23-27.



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