Acta Scientific Pharmaceutical Sciences (ASPS)(ISSN: 2581-5423)

Research Article Volume 4 Issue 3

Antioxidant Properties of Some Nigerian Green Leafy Vegetables

Samuel GA*, Dosunmu MI and Okon JA

Department of Pure and Applied Chemistry, University of Calabar, Calabar, Nigeria

*Corresponding Author: Samuel GA, Department of Pure and Applied Chemistry, University of Calabar, Calabar, Nigeria.

Received: December 23, 2019; Published: February 27, 2020



  The anti-oxidant properties of acetone extracts of bitter leaf (Vernonia anygdalina), lemon basil (Ocimun citriodorum, candle bush (Senna alata) “Afang” (Gnetum africanum) and rat ear (Portulaca oleracea) were investigated using 2,2- diphenyl-1-picrylhydrazyl (DPPH) radical scavenging method. The results obtained from the analysis of 1 mg/ml of five vegetable extracts indicate that Vernonia anygdalina (VA), Senna alata (SA), Gnetum africanum (GA), Ocimun citriodorum (OC) and Portulaca oleracea (PO) contain(s) 89.30%, 86.70%, 92.00%, 75.60% and 95.00% inhibition of DPPH respectively. In comparison with the reference sample {ascorbic (AA) acid and BHT which had 100% inhibition at 1 mg/ml}, it was observed that all the green vegetables investigated had a remarkably high antioxidant inhibition properties in DPPH. Portulaca oleracea had the highest DPPH inhibition property (95.00%) compared with Ocimum citrodorum which exhibited the least inhibition property of 75.00%. The degree of the inhibition property follows the order: PO > GA > VA > SA > OC. The range of DPPH inhibition for all the vegetables from highest to lowest was known to be 95% - 75% with 20% fundamental interval. The low range implies that antioxidant properties of all the vegetable are remarkably close.

Keywords: Antioxidant; Green Vegetables; Scavenging Method; Acetone Extracts



  1. Sies H., et al. “Nutritional, dietary and postprandial oxidative stress”. Journal of Nutrition (2005).
  2. Zheng W and Wang S. “Antioxidant activities and phenolic compound in selected Herbs”. American Chemical Society, Maryland (2001).
  3. Sies H. “Physiological society symposium impaired endothelial and smooth muscle cell function in oxidative stress”. Experimental Physiology, Great Britain (1997).
  4. Imaga N and Bamigbeta N. “In vivo Biochemical assessment of the aqueous extracts of Vernonia amygdalina”. International Journal of Nutrition and Metabolism, Lagos (2013). 
  5. Ayoola G., et al. “Phytochemical and antioxidant screening of some plant of apocynacea from South West Nigerian”. African Journal of Plant Science 2 (2008).
  6. Erasto P., et al. “Antioxidant constituent of Vernonia amygdalina leave”. Pharmceutical Biology, Chicago (2007).
  7. Hamzah U., et al. “Antioxidant properties of selected African, vegetables, fruits and mushrooms; A Review”. Intech Open Science/Open minds, Minna (2013).
  8. Aguwa C. “Diabetes mellitus in therapeutic basic of clinical pharmacy in the tropics”. Optimal Publisher, Enugu, Nigeria (2006).
  9. Chatterjee S., et al. “Study of antioxidant activity and immune stimulating potency of the ethnomedicinal plant of Cassia alata”. Open Access Journal (2013).
  10. Mortada M., et al. “Evaluation of antioxidant and antimicrobial of certain cassia species”. Australian Journal of Basic and Applied Science, Giza (2011).
  11. Saito S., et al. “Australian from Cassia alata induces DNA adducts in vivo and repairable DNA damage yeast Saccharomyces cerevisiae”. Experimental Physiology, Great Britain (2012).
  12. Ekpo A. “Determination of chemistry composition of Gnetum africanum seeds”. Pakistan Journal of Nutrition. Faisalabail (2007).
  13. Orwa C., et al. “Agroforestry’s database, A tree Reference and selection Guide version 4.0” (2009). 
  14. Janarthan B and Sumathi E. “Planted regeneration from nodal explants of Ocinum citriodorum, Banglaesh”. Journal of Scientific and Industrial Research, Alwarthiru-Nagar (2012).
  15. Eisen B., et al. “Stability of isoflavones in milk stored at elevated and ambient temperature”. Journal in Agricultural Food Chemistry 51 (2003): 2212-2215.
  16. Ferreira D., et al. “Composition of phenolic compounds in a Portuguese peer (Pyruscommunis) and changes after sun-drying”. Journal in Agricultural Food Chemistry 50 (2002): 4537-4544.
  17. Re R., et al. “Effect of food processing on flavonoid and lycopene status in a Mediterranean tomatoes variety free radical resources”. Critical Reviewers in Food Science and Nutrition 36 (2002): 803-810.
  18. Charles M. “Elevating antioxidant levels in food through organic farming and food processing”. The Organic Center for Education and Promotion (2005).
  19. Tokeoka G., et al. “Processing effects on lycopene content and antioxidant activity of tomatoes”. Journal Agricultural Cultural Food Chemistry 49 (2001): 3714-3717.
  20. Dewanto V., et al. “Thermal processing enhances the nutritional values of tomatoes by increasing total antioxidant activity”. Journal in Agricultural Food Chemistry 50 (2002): 3010-3014.
  21. Agbogidi O and Akpomorine M. “Health and Nutritional Benefit of bitter leaf (Vernonia amgydaline)”. International Journal of Foods and Agricultural Research (2013).
  22. Ali F., et al. “Gneturn africanum, A wild food plant from the African with many nutritional and medicinal purposes”. Journal of Medical Food (2011).
  23. Aliyu H and Morufu A. “Proximate analysis of some leafy vegetables (roselle, jute and bitterleaf)”. International Journal of Food and Agricultural Research (2006).
  24. Aruma O. “Methodological consideration for characterizing potential antioxidant action of bioactive component in food planta”. Lbadan (2003).
  25. Association of the Official Analytical Chemists [AOAC], Antioxidant determination using official method of analysis, 16th edition, Washington D.C (2000).
  26. Dosunmu M and Bassey F. “Chemistry composition and functional properties Musa sapientum (cooking Banana) Flour”. Natural and Applied Science Transaction, Landel publishers 3 (2001): 65-69.
  27. Eisen B., et al. “Stability of isoflavones in soy milk stored at elevated and ambient temperature”. Journal in Agricultural Food Chemistry 51 (2003): 2212-2215.
  28. Jimoh F., et al. “Comparison of the nutritive value, antioxidant and anti-bacteria activities of Sonchus asper and Sonchus oleraceus”. Academy of Chemistry of Globe Publication, Fort Hare (2010).
  29. Nwanjo H. “Effacacy of aqueous leaf extract of vernonia amygdalina on plasma lipoprotein and oxidative status in diabetic rat models”. Nigeria Journal of Physiological Science, Owerri (2005).
  30. Omorwegie S and Osagie A. “Antioxidant properties of methanolic extracts of some Nigeria plants on Nutritionally Stress Rats”. Nigerian Journal of Basic and Applied Science (2012).


Citation: Samuel GA., et al. “Antioxidant Properties of Some Nigerian Green Leafy Vegetables". Acta Scientific Pharmaceutical Sciences 4.3 (2020): 36-40.

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