Acta Scientific Pharmaceutical Sciences (ISSN: 2581-5423)

Research Article Volume 4 Issue 1

TCF7L2 Polymorphism and Inflammation in Type II Diabetes: A Nutrigenetic Pilot Study

Mahmoud Ibrahim Mohamed Hassanein Elghazawy1*, Laila Abdel Aala ElShawarby2, Rasha Mohamed Mamdouh Abdou Saleh3 and Nermeen Tayseer Aly Fouad3

1Faculty of Medicine, Ain Shams University, Egypt
2Professor of Clinical and Chemical Pathology, Faculty of Medicine, Ain Shams University, Egypt
3Assistant Professor of Clinical and Chemical Pathology, Faculty of Medicine, Ain Shams University, Egypt

*Corresponding Author: Mahmoud Ibrahim Mohamed Hassanein Elghazawy, Faculty of Medicine, Ain Shams University, Egypt.

Received: November 25, 2019; Published: December 06, 2019

×

Abstract

  Though the genetic susceptibility to T2D is polygenic, with almost 50 loci identified to be associated with T2D risk, single nucleotide polymorphisms (SNP) at the transcription factor 7-like 2 gene (TCF7L2) have been strongly and consistently associated with T2D risk in various populations and ethnic groups. The exact mechanism by which TCF7L2 variations predispose to T2D is not clear. TCF7L2, which spans a 215 863 base-pair region on chromosome 10q25.3, encodes the transcription factor TCF7L2 that is involved in the Wnt signaling pathway, which seems to be critical to pancreatic islet development and adipogenesis through the dysregulation of proglucagon gene expression. Type 2 diabetes is reported to be associated with a systemic low-grade inflammation as indicated by increased levels of circulating acute-phase proteins like CRP and of IL-6. Scientific researchers believe that through exploiting the recent genomic information and better understanding of nutrient-gene interactions, better health outcomes can be achieved if nutritional requirements are customized for each individual taking into consideration the inherited genetic characteristics depending on life stage, dietary preferences and health status.

Subjects: Cases group: 25 patients diagnosed with Type II Diabetes Mellitus selected from the outpatient clinics of the National Nutrition Institute and Ain Shams University Hospitals. Controls group: 25 age and sex matched, apparently healthy patients’ relatives, with no known history of Diabetes.

Methods: Full demographic and clinical data will be collected from patients’ data base. Then all individuals included in this study were subjected to a peripheral whole blood on EDTA and serum samples will be obtained and the following laboratory investigations will be performed: 1-Random blood glucose. 2-Glycated Hemoglobin (HbA1C). 3- Genotyping of TCF7L2 gene polymorphism by Real time PCR (Polymerase Chain Reaction). 4-Interleukin 6 (IL6) Assay by ELISA.

Results: There was a significant positive correlation between levels of serum IL-6 and dietary fat intake in the case group; however, the same correlation was not statistically significant with caloric or carbohydrate intakes. There was no significant correlations between IL-6 and either caloric intake, carbohydrate intake, or fat intake in the control group. No significant correlation between TCF7L2 genotypes and the levels of IL-6 in the in the cases group. Correlation between levels of IL-6 and the cycle threshold (ct) of the risk (T) allele (indicating allele expression) shows no statistical significance in cases or control group.

Keywords: Type 2 Diabetes; TCF7L2; Cycle Threshold

×

References

  1. Silveira Assmann T., et al. “Polymorphisms in the TLR3 gene are associated with risk for type 1 diabetes mellitus”. European Journal of Endocrinology10 (2014): 519-527.
  2. Balducci Stefano., et al. “Physical Exercise as therapy for type II diabetes”. Diabetes/Metabolism Research and Reviews 30 (2014): 13-23.
  3. Fawcett KA and Barroso I. “The genetics of obesity: FTO leads the way”. Trends in Genetics6 (2010): 266-274.
  4. Finucane MM., et al. “National, regional, and global trends in body-mass index since 1980: Systematic analysis of health examination surveys and epidemiological studies with 960 country-years and 9??1 million participants”. The Lancet9765 (2011): 557-567.
  5. Frayling TM., et al. “A Common Variant in the FTO Gene Is Associated with Body Mass Index and Predisposes to Childhood and Adult Obesity”. Science 316 (2007): 889-893.
  6. Gabay C. “Interleukin-6 and chronic inflammation”. Arthritis Research and Therapy 8 (2006): S3.
  7. Gjesing AP., et al. “Carriers of the TCF7L2 rs7903146 TT genotype have elevated levels of plasma glucose, serum proinsulin and plasma gastric inhibitory polypeptide (GIP) during a meal test”. Diabetologia 54 (2011): 103-110.
  8. Köttgen A., et al. “TCF7L2 variants associate with CKD progression and renal function in population-based cohorts”. Journal of the American Society of Nephrology: JASN 19 (2008): 1989-1999.
  9. Kristiansen OP and Mandrup-Poulsen T. “Interleukin-6 and Diabetes”. Diabetes 54 (2005): S114 LP-S124.
  10. Kwon H and Pessin JE. “Adipokines mediate inflammation and insulin resistance”. Frontiers in Endocrinology 4 (2013): 1-13.
  11. Lovejoy JC., et al. “Increased visceral fat and decreased energy expenditure during the menopausal transition”. International Journal of Obesity6 (2008): 949-958.
  12. Lyssenko V., et al. “Mechanisms by which common variants in the TCF7L2 gene increase risk of type 2 diabetes”. Journal of Clinical Investigation8 (2007): 2155-2163.
  13. MacDonald BT., et al. “Wnt/??-Catenin Signaling: Components, Mechanisms, and Diseases”. Developmental Cell 17 (2009): 9-26.
  14. Manuscript A. “Adipokines in inflammation and metabolic disease”. Nature Review Immunology2 (2011): 85-97.
  15. Pineda-Tenor D., et al. “rs7903146 Polymorphism at Transcription Factor 7 Like 2 Gene Is Associated with Total Cholesterol and Lipoprotein Profile in HIV/Hepatitis C Virus-Coinfected Patients”. AIDS Research and Human Retroviruses3 (2015): 326-334.
  16. Prestwich TC and MacDougald OA. “Wnt/β-catenin signaling in adipogenesis and metabolism”. Current Opinion in Cell Biology6 (2007): 612-617.
  17. Rabe K and Lehrke M. “Adipokines and Insulin Resistance”. Molecular Medicine 14 (2008): 1.
  18. Rao TP and Kühl M. “An updated overview on wnt signaling pathways: A prelude for more”. Circulation Research12 (2010): 1798-1806.
  19. Sindhu S., et al. “Obesity is a positive modulator of IL-6R and IL-6 expression in the subcutaneous adipose tissue: Significance for metabolic inflammation”. PLoS ONE7 (2015): 1-17.
  20. Stančáková A and Laakso M. “Genetics of type 2 diabetes”. Endocrine Development4 (2018): 203-220.
  21. Works C., et al. Chapter 10. Free Press 110 (2006).
  22. Zhai L., et al. “Role of reactive oxygen species in injury-induced insulin resistance”. Molecular Endocrinology (Baltimore, Md.) 25 (2011): 492-502.
×

Citation

Citation: Mahmoud Ibrahim Mohamed Hassanein Elghazawy., et al. “TCF7L2 Polymorphism and Inflammation in Type II Diabetes: A Nutrigenetic Pilot Study".Acta Scientific Pharmaceutical Sciences 4.1 (2020): 02-08.




Metrics

Acceptance rate32%
Acceptance to publication20-30 days

Indexed In




News and Events


  • Certification for Review
    Acta Scientific certifies the Editors/reviewers for their review done towards the assigned articles of the respective journals.
  • Submission Timeline for Upcoming Issue
    The last date for submission of articles for regular Issues is September 25, 2024.
  • Publication Certificate
    Authors will be issued a "Publication Certificate" as a mark of appreciation for publishing their work.
  • Best Article of the Issue
    The Editors will elect one Best Article after each issue release. The authors of this article will be provided with a certificate of "Best Article of the Issue"
  • Welcoming Article Submission
    Acta Scientific delightfully welcomes active researchers for submission of articles towards the upcoming issue of respective journals.

Contact US