Pritam S Jain*, Swati D Yeole, Najeef A Ansari and Sanjay J Surana
R. C. Patel Institute of Pharmaceutical Education and Research, Karwand Naka, Shirpur, India
*Corresponding Author: Pritam S Jain, R. C. Patel Institute of Pharmaceutical Education and Research, Karwand Naka, Shirpur, India.
Received: April 06, 2018; Published: May 29, 2018
Citation: Pritam S Jain., et al. “Quantitative Estimation of Carfilzomib by UV-AUC Method”. Acta Scientific Pharmaceutical Sciences 2.6 (2018).
Aim: The aim of this work is to establish a novel, simple, precise and sensitive UV-AUC spectrophotometric method has been developed for estimation of Carfilzomib from bulk and in-house formulation. Moreover, this work is performed to validate as per ICH guidelines.
Materials and Methods: Carfilzomib working standard was obtained as gift sample from Cipla Pharmaceuticals, Mumbai. In Methods, a stock standard solution was prepared by dissolving 100 mg of Carfilzomib in 100 mL of ethanol to obtain a concentration of 1000 μg/mL. After suitable dilution, 100 μg/mL of Carfilzomib prepared and scanned in the UV-visible range 400 - 200 nm; Carfilzomib showed a maximum wavelength at 258 nm. The Method applied was area under curve (AUC in which area under curve was integrated in the wavelength range of 256.80 - 261.20.
Results: The drug follows linearity in the concentration range 100 - 600 μg/mL with correlation coefficient value 0.999. The developed method was implemented to the marketed formulation and the evaluated % amount of drug was found in good consistency with the label claim.
Conclusion: The proposed method was a rapid, economical and commercial quality-control tool for routine analysis of Carfilzomib in bulk and in pharmaceutical formulations.
Keywords: Carfilzomib; UV Method; Validation; Quantitative Determination
Copyright: © 2018 Pritam S Jain., et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.