Nita Yadav¹* and Anju Goyal²

¹Department of Pharmacy, SRMSCET, Bareilly, Uttar Pradesh, India
²B. N. Institute of Pharmaceutical Sciences, Udaipur, Rajasthan, India

*Corresponding Author: Nita Yadav, Department of Pharmacy, SRMSCET, Bareilly, Uttar Pradesh, India.

Received: December 23, 2017; Published: January 30, 2018

Citation: Nita Yadav and Anju Goyal. “Dual Wavelength Method for Simultaneous Estimation of Aliskiren and Amlodipine in Combined Tablet Formulation”. Acta Scientific Pharmaceutical Sciences 2.2 (2018).

Abstract

  The renin inhibitor aliskiren was determined spectrophotometrically with amlodipine in its combined tablet formulation. The UV-spectrophotometric method utilized dual wavelength method for simultaneous estimation of aliskiren and amlodipine. From the overlain spectra of two drugs, the set of two wavelengths λ₁ (235.6 nm) and λ₂ (242.2 nm) were selected for estimation of aliskiren. Amlodipine was estimated directly at 365 nm as a single component without any interference, as aliskiren has zero absorbance at this wavelength. Developed method obeys Beer’s law in the concentration range used for estimation of aliskiren and amlodipine. The mean percentage label claim of aliskiren and amlodipine using dual wavelength method was found to be 99.90 and 99.85, respectively. Accuracy of the method was found between 100.20 - 100.65%.

Keywords: Aliskiren; Amlodipine; UV-Spectrophotometric Method; Dual Wavelength Method

Copyright: © 2018 Nita Yadav and Anju Goyal. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.