Acta Scientific Pharmaceutical Sciences

Research ArticleVolume 1 Issue 6

Broad Antibacterial Spectrum and High Performance Liquid Chromatography Profiles of Ocimum sanctum Leaf Extract

Malay Kr Das1, Manisha Mandal2 and Shyamapada Mandal2*

1Department of Zoology, University of Gour Banga, Malda, West Bengal, India
2Department of Physiology, MGM Medical College and LSK Hospital, Kishanganj, India

*Corresponding Author: Shyamapada Mandal, Laboratory of Microbiology and Experimental Medicine, Department of Zoology, University of Gour Banga, Malda, West Bengal, India. E-mail:

Received: September 23, 2017; Published: December 06, 2017

Citation: Shyamapada Mandal., et al. “Broad Antibacterial Spectrum and High Performance Liquid Chromatography Profiles of Ocimum sanctum Leaf Extract”. Acta Scientific Pharmaceutical Sciences 1.6 (2018).


  Medicinal plants are the promising source of bioactive compounds which are well known for their several biological activities including antibacterial property. The current study assesses the antibacterial activity of two indigenous varieties (Dark and Bright) of Ocimum sanctum leaf extracts against gram-positive and gram-negative clinical bacteria, and determines the HPLC profiles of the extracts. Following disc diffusion method, the antibacterial activity of ethanolic leaf extracts (DOSE and BOSE) and aqueous leaf extracts (AqDOS and AqBOS) of both varieties O. sanctum, was determined and their phytochemical analysis was performed by HPLC. The HPLC chromatogram showed the presence of 11, in DOSE, and 9, in BOSE, major compounds with the RTs 1.77 - 5.29 min and 1.92 - 6.27 min, respectively, while both AqDOS and AqBOS had 4 major compounds, with the respective RTs of 1.66 - 6.17 min and 1.64 - 2.51 min. The ZDI values, due to the action of DOSE (6.25 mg), were recorded as 20 mm, for gram-positive bacteria, and 20 - 24 mm, for gram- negative bacteria, while BOSE (6.25 mg) had ZDIs 17 - 19 mm and 14 - 24 mm, respectively, for gram-positive and gram-negative bacteria. The DOSE and BOSE were more active compared to the AqDOS and AqBOS, in terms of the antibacterial activity by disc diffusion. Thus, the O. sanctum leaves might be utilized as the source of biotherapeutic agents to be administered against broad range of bacterial infections.

Keywords: Ocimum sanctum; Broad Spectrum Antibacterial Activity; Zone Diameter of Inhibition; Phytocomponents; High Performance Liquid Chromatography

Copyright: © 2018 Shyamapada Mandal., et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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