Strategic Design and Fabrication of Nanofibrous Scaffolds for Articular Cartilage Repair
Soheila Zamanlui1, Maryam Bikhof Torbati2, Leila Mohammadi
Amirabad3, Abdolreza Ardeshirylajimi3, Simzar Hosseinzadeh3, Masoud Soleimani4,5 and Shahab Faghihi1
1Stem cell and regenerative medicine group, National Institute of Genetic Engineering and Biotechnology (NIGEB), Iran
2Department of Biology, Yadegar-e-Imam Khomeini (RAH) Shahr-e-Rey Branch, Islamic Azad University, Tehran, Iran
3School of Advanced Technologies in Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran
4Department of Hematology, Faculty of Medical Sciences, Tarbiat Modares University, Iran
5Nanotechnology and Tissue Engineering Department, Stem Cell Technology Research Center, Iran
*Corresponding Author: Shahab Faghihi, Stem cell and regenerative medicine group, National Institute of Genetic Engineering and Biotechnology (NIGEB), Iran.
May 23, 2022; Published: December 13, 2022
The loss of articular cartilage is a common defect occurs by accidents, disease, sport injuries and gradual wears; however, it won’t be spontaneously repaired as it is an avascular tissue. Recently, cartilage tissue engineering has suggested attractive strategy for regeneration of cartilage tissue. Here, aligned nanofibrous PCL/PLGA scaffolds are fabricated to mimic the aligned fibers of the extracellular matrix of the superficial zone. Bone marrow-derived mesenchymal stem cells (BMMSCs) along with transforming growth factor β1 (TGFβ1), insulin-transferrin-selenium (ITS), ascorbic acid, and dexamethasone are seeded on the scaffolds to investigate the chondrogenesis efficiency using histology and real-time PCR. The expression levels of collagen II and aggrecan are detected which reveal a higher level of type II collagen compared to aggrecan in the presence of differentiating medium. Moreover, the expression of collagen II and aggrecan is significantly higher in the presence of differentiation medium rather than the group without signaling factors. The surface morphology and signaling factors have dominant role in upregulated expression of aggrecan and collagen II, respectively. It is believed that simultaneous application of signaling molecules and aligned nanofibrous PCL/PLGA scaffold enhances the efficiency of chondrogenesis by recapitulating the conditions in the superficial zone of cartilage.
Keywords: Articular Cartilage; Cartilage Tissue Engineering; Aligned Nanofibrous Scaffolds; Signaling Factors; Chondrogenesis
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