Acta Scientific Orthopaedics (ISSN: 2581-8635)

Research Article Volume 6 Issue 1

Strategic Design and Fabrication of Nanofibrous Scaffolds for Articular Cartilage Repair

Soheila Zamanlui1, Maryam Bikhof Torbati2, Leila Mohammadi Amirabad3, Abdolreza Ardeshirylajimi3, Simzar Hosseinzadeh3, Masoud Soleimani4,5 and Shahab Faghihi1

1Stem cell and regenerative medicine group, National Institute of Genetic Engineering and Biotechnology (NIGEB), Iran 2Department of Biology, Yadegar-e-Imam Khomeini (RAH) Shahr-e-Rey Branch, Islamic Azad University, Tehran, Iran 3School of Advanced Technologies in Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran 4Department of Hematology, Faculty of Medical Sciences, Tarbiat Modares University, Iran 5Nanotechnology and Tissue Engineering Department, Stem Cell Technology Research Center, Iran

*Corresponding Author: Shahab Faghihi, Stem cell and regenerative medicine group, National Institute of Genetic Engineering and Biotechnology (NIGEB), Iran.

Received: May 23, 2022; Published: December 13, 2022


The loss of articular cartilage is a common defect occurs by accidents, disease, sport injuries and gradual wears; however, it won’t be spontaneously repaired as it is an avascular tissue. Recently, cartilage tissue engineering has suggested attractive strategy for regeneration of cartilage tissue. Here, aligned nanofibrous PCL/PLGA scaffolds are fabricated to mimic the aligned fibers of the extracellular matrix of the superficial zone. Bone marrow-derived mesenchymal stem cells (BMMSCs) along with transforming growth factor β1 (TGFβ1), insulin-transferrin-selenium (ITS), ascorbic acid, and dexamethasone are seeded on the scaffolds to investigate the chondrogenesis efficiency using histology and real-time PCR. The expression levels of collagen II and aggrecan are detected which reveal a higher level of type II collagen compared to aggrecan in the presence of differentiating medium. Moreover, the expression of collagen II and aggrecan is significantly higher in the presence of differentiation medium rather than the group without signaling factors. The surface morphology and signaling factors have dominant role in upregulated expression of aggrecan and collagen II, respectively. It is believed that simultaneous application of signaling molecules and aligned nanofibrous PCL/PLGA scaffold enhances the efficiency of chondrogenesis by recapitulating the conditions in the superficial zone of cartilage.

Keywords: Articular Cartilage; Cartilage Tissue Engineering; Aligned Nanofibrous Scaffolds; Signaling Factors; Chondrogenesis


  1. Huber M., et al. “Anatomy, biochemistry, and physiology of articular cartilage”. Investigative Radiology10 (2000): 573-580.
  2. Estes BT., et al. “Articular cartilage repair”. Google Patents (2015).
  3. Makris EA., et al. “Repair and tissue engineering techniques for articular cartilage”. Nature Reviews Rheumatology1 (2015): 21-34.
  4. Mohammadi Amirabad L., et al. “Enhanced Cardiac Differentiation of Human Cardiovascular Disease Patient-Specific Induced Pluripotent Stem Cells by Applying Unidirectional Electrical Pulses Using Aligned Electroactive Nanofibrous Scaffolds”. ACS Applied Materials and Interfaces 8 (2017): 6849-6864.
  5. Bianco P. “Mesenchymal” stem cells”. Annual Review of Cell and Developmental Biology 30 (2014): 677-704.
  6. Amari A., et al.In vitro generation of IL-35-expressing human Wharton's Jelly-derived mesenchymal stem cells using lentiviral vector”. Iranian Journal of Allergy, Asthma and Immunology4 (2015): 416.
  7. Kozhemyakina E., et al. “A pathway to bone: signaling molecules and transcription factors involved in chondrocyte development and maturation”. Development5 (2015): 817-831.
  8. Rajaei B., et al. “Pancreatic Endoderm‐Derived From Diabetic Patient‐Specific Induced Pluripotent Stem Cell Generates Glucose‐Responsive Insulin‐Secreting Cells”. Journal of Cellular Physiology10 (2016): 2616-2625.
  9. Boeuf S and W Richter. “Chondrogenesis of mesenchymal stem cells: role of tissue source and inducing factors”. Stem Cell Research and Therapy4 (2010): 31.
  10. Watabe T and K Miyazono. “Roles of TGF-β family signaling in stem cell renewal and differentiation”. Cell Research1 (2009): 103-115.
  11. Liu X., et al. “Role of insulin-transferrin-selenium in auricular chondrocyte proliferation and engineered cartilage formation in vitro”. International Journal of Molecular Sciences1 (2014): 1525-1537.
  12. Chua K., et al. “Insulin-transferrin-selenium prevent human chondrocyte dedifferentiation and promote the formation of high quality tissue engineered human hyaline cartilage”. European Cells and Materials 9 (2005): 58-67.
  13. Yang YH and GA Barabino. “Requirement for serum in medium supplemented with insulin-transferrin-selenium for hydrodynamic cultivation of engineered cartilage”. Tissue Engineering Part A15-16 (2011): 2025-2035.
  14. Clark AG., et al. “The effects of ascorbic acid on cartilage metabolism in guinea pig articular cartilage explants”. Matrix Biology2 (2002): 175-184.
  15. Leboy PS., et al. “Ascorbic acid induces alkaline phosphatase, type X collagen, and calcium deposition in cultured chick chondrocytes”. Journal of Biological Chemistry29 (1989): 17281-17286.
  16. Shintani N and EB Hunziker. “Differential effects of dexamethasone on the chondrogenesis of mesenchymal stromal cells: influence of microenvironment, tissue origin and growth factor”. Eur Cell Mater 22 (2011): 302-320.
  17. Zamanlui S., et al. “Enhanced chondrogenic differentiation of human bone marrow mesenchymal stem cells on PCL/PLGA electrospun with different alignment and composition”. Polymeric Materials and Polymeric Biomaterials1 (2017).
  18. Cao Z., et al. “Scaffolding biomaterials for cartilage regeneration”. Journal of Nanomaterials 2014 (2014): 4.
  19. Gentile P., et al. “An overview of poly (lactic-co-glycolic) acid (PLGA)-based biomaterials for bone tissue engineering”. International Journal of Molecular Sciences3 (2014): 3640-3659.
  20. Oraee-Yazdani S., et al. “Co-transplantation of autologous bone marrow mesenchymal stem cells and Schwann cells through cerebral spinal fluid for the treatment of patients with chronic spinal cord injury: safety and possible outcome”. Spinal cord (2015).
  21. Hosseinzadeh S., et al. “The nanofibrous PAN-PANi scaffold as an efficient substrate for skeletal muscle differentiation using satellite cells”. Bioprocess and Biosystems Engineering (2006): 1-10.
  22. Wang X., et al. “High flux filtration medium based on nanofibrous substrate with hydrophilic nanocomposite coating”. Environmental Science and Technology19 (2005): 7684-7691.
  23. Amirabad LM., et al. “Significant changes of 5-hydroxytriptamine 3A receptor gene expression in peripheral blood mononuclear cells of allergic asthmatic patients”. Iranian Journal of Allergy, Asthma and Immunology1 (2014): 33.
  24. Fung YC. “Biomechanics: mechanical properties of living tissues”. Springer Science and Business Media (1981).
  25. Simmons CA., et al. “Cyclic strain enhances matrix mineralization by adult human mesenchymal stem cells via the extracellular signal-regulated kinase (ERK1/2) signaling pathway”. Journal of Biomechanics8 (2003): 1087-1096.
  26. Hellingman CA., et al. “Smad signaling determines chondrogenic differentiation of bone-marrow-derived mesenchymal stem cells: inhibition of Smad1/5/8P prevents terminal differentiation and calcification”. Tissue Engineering Part A7-8 (2011): 1157-1167.
  27. Davidson ENB., et al. “Increase in ALK1/ALK5 ratio as a cause for elevated MMP-13 expression in osteoarthritis in humans and mice”. The Journal of Immunology12 (2009): 7937-7945.
  28. Maldonado M and J Nam. “The role of changes in extracellular matrix of cartilage in the presence of inflammation on the pathology of osteoarthritis”. BioMed Research International (2013).
  29. Davidson ENB., et al. “Correction: Increase in ALK1/ALK5 Ratio as a Cause for Elevated MMP-13 Expression in Osteoarthritis in Humans and Mice”. The Journal of Immunology4 (2010): 2629-2629.


Citation: Shahab Faghihi., et al. “Strategic Design and Fabrication of Nanofibrous Scaffolds for Articular Cartilage Repair”.Acta Scientific Orthopaedics 6.1 (2023): 117-125.


Copyright: © 2023 Shahab Faghihi., et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.


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