Acta Scientific Orthopaedics (ISSN: 2581-8635)

Research Article Volume 4 Issue 8

Polymorphism of Pro and Anti-Inflammatory Cytokine Genes in Children with Legg-Calve-Perthes Disease

Shabaldin NA1*, Shabaldin AV1, Tsepokina AV2, Shabaldina EV1 and Rovda Yu I1

1Kemerovo State Medical University, Kemerovo, Russian Federation
2Scientific-Research Institute of Complex Problems of Cardiovascular Diseases, Kemerovo, Russian Federation

*Corresponding Author: Shabaldin NA, Department of Pediatric Surgery, Kemerovo State Medical University, Kemerovo, Russian Federation.

Received: July 02, 2021; Published: July 21, 2021

Abstract

Introduction: The pathogenesis of Perthes disease is being actively studied. One of the possible mechanisms that cause osteodestruction in the early stages of Perthes disease is the predominance of the biological activity of pro-inflammatory cytokines. The association of polymorphic variants of cytokine genes may be of particular importance in this case.

Aim: Search for associations of Perthes disease with polymorphic variants of pro-inflammatory and anti-inflammatory cytokine genes.

Materials and Methods: Polymorphic variants of pro-inflammatory and anti-inflammatory cytokine genes were studied: IL10 (rs1800896), IL13 (RS20541), IL18 (RS187238), IL18 (RS5744292), IL1A (RS1800587), IL1Rn (POL_GF_58), IL1Rn (RS4251961), IL1β (RS16944), IL1β (RS1143634), IL4 (POL_GF_99), IL4 (rs2243250), IL6 (rs1800796), IL6 (rs1800795), INFG (rs2430561), TGFB (rs1800469), TNFα (rs1800629). The main group consisted of 25 children with 2,3 stages of Perthes disease according to the Reintberg classification, the control group consisted of 40 conditionally healthy children.

Results: The highest predictor coefficient was found for the polymorphic variant of the IL10 gene (rs1800896). The second in predictor significance was the mutant homozygous IL6 genotype (rs1800796). The third significant predictor of BLCP was the homozygous mutant genotype INFG (rs2430561). The protective genotype was located in the polymorphic region of the TNFA gene (rs1800629). Based on the logistic regression, an equation was obtained for calculating the risk of LCPD formation based on genetic predictors with potentiating and protective properties.

Conclusion: The potentiating genotypes for the risk of LCPD formation were IL6 (rs1800796), INFG (rs2430561) and IL10 (rs1800896). The genotype of the protective risk of the formation of LCPD - TNFα (rs1800629) was identified. It was shown that the combination of genotypes of pro-inflammatory and anti-inflammatory cytokines (IL6 (rs1800796), INFG (rs2430561), IL10 (rs1800896), TNFα (rs1800629), TGFB (rs1800469) and IL1β (rs16944)) make the most significant contribution to the determination of LCPD in children.

Keywords: Perthes Disease; Cytokines; Cytokine Gene Associations

References

  1. Kim HKW. “Pathophysiology and New Strategies for the Treatment of Legg-Calve-Perthes Disease”. The Journal of Bone and Joint Surgery7 (2012): 659-669.
  2. Adapala NS., et al. “Necrotic Bone Stimulates Proinflammatory Responses in Macrophages through the Activation of Toll-Like Receptor 4”. The American Journal of Pathology11 (2016): 2987-2999.
  3. Yamaguchi R., et al. “HIF-1-Dependent IL-6 Activation in Articular Chondrocytes Initiating Synovitis in Femoral Head Ischemic Osteonecrosis”. The Journal of Bone and Joint Surgery3 (2016): 1122-1131.
  4. Patel NM and Feldman DS. “Biologic and Pharmacologic Treatment of Legg-Calvé-Perthes Disease”. Legg-Calvé-Perthes Disease (2020): 181-194.
  5. Akbarian-Bafghi MJ., et al. “Association of IL-6 -174G > C and -572G > C Polymorphisms with Risk of Legg-Calve-Perthes Disease in Iranian Children”. Fetal and Pediatric Pathology (2019).
  6. Meiss Al., et al. “Denosumab and surgery for the treatment of Perthes’ disease in a 9-year-old boy: favorable course documented by comprehensive imaging — a case report”. Acta Orthopaedica (2017).
  7. N Kamiya., et al. “IL6 receptor blockade preserves articular cartilage and increases bone volume following ischemic osteonecrosis in immature mice”. Osteoarthritis and Cartilage 2 (2019): 326-335.
  8. Fiorentino DF., et al. “IL-10 inhibits cytokine production by activated macrophages”. The Journal of Immunology 11 (1991): 3815-3822.
  9. Fernandes MT., et al. “Association of interleukin-6 gene polymorphism (rs1800796) with severity and functional status of osteoarthritis in elderly individuals”. Cytokine2 (2015): 316-320.
  10. Gomes da Silva IIF., et al. “IL1β, IL18, NFKB1 and IFNG gene interactions are associated with severity of rheumatoid arthritis: A pilot study”. Autoimmunity 2 (2020): 95-101.
  11. Ahmed R., et al. “Effect of TNF-α -308G/A (rs1800629) promoter polymorphism on the serum level of TNF-α among Iraqi patients with generalized vitiligo”. Clinical, Cosmetic and Investigational Dermatology 13 (2020): 825-835.

Citation

Citation: Shabaldin NA., et al. “Polymorphism of Pro and Anti-Inflammatory Cytokine Genes in Children with Legg-Calve-Perthes Disease".Acta Scientific Orthopaedics 4.8 (2021): 45-51.

Copyright

Copyright: © 2021 Shabaldin NA., et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.




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