Elif Sibel Aslan¹, Hakan Er², Mete Emir Ozgurses³, Medine Tasdemir⁴, Sukru Ozen⁶, Sajjad Eslamkhah¹ and Nazli Ece Gungor-Ordueri⁷*

¹Molecular Biology and Genetics Department, Life Sciences and Engineering Faculty, Biruni University, Istanbul, Turkey
²Biophysics Department, Akdeniz University, Antalya, Turkey
³University of Illinois Chicago College of Medicine, Department of Physiology and Biophysics, Chicago, IL, USA
⁴The University of Chicago Medical Center, Department of Obstetrics and Gynecology, Chicago, IL, USA
⁵Urology Department, Bilgi University, Istanbul, Turkey
⁶Electric and Electronic Department, Engineering Faculty, Akdeniz University, Antalya, Turkey
⁷Biruni University, Medicine Faculty, Department of Histology and Embryology, Istanbul, Turkey

*Corresponding Author: Nazli Ece Ordueri, Associate Professor, Biruni University, Medicine Faculty, Department of Histology and Embryology, Istanbul, Turkey.

Received: June 07, 2024; Published: July 09, 2024

Abstract

The use of devices that generate electromagnetic fields (EMF), such as cell phones, is on the rise and has sparked interest in studying their effects of human health. We investigated of the proteins found in the entire testes of adult male Wistar rats that were exposed to EMF radiation at frequencies of 900 MHz and 2150 MHz. The exposure lasted for 2 hours per day over a period of 1 week and 10 weeks, which simulated various levels of cell phone use in humans. After the experiment, the subjects were sacrificed and testicular samples were collected Consider specifying the measurement technique used to assess the levels of N-cadherin and other proteins, such as immunoblotting or immunofluorescence, a protein that regulates the blood testis barrier (BTB). Immunohistochemistry was also used to identify other proteins involved in BTB regulation, namely Occluding, F-actin, Ezrin, and Fascin, and to confirm any protein misfolding in the BTB area. Our findings indicate that exposure to radio frequency modulated EMFs has significant effects on the BTB, specifically on the protein composition in the testes of rodents. The results suggest that 1 week and 10 weeks of EMF radiation exposure induce stress in the testicular tissue, potentially leading to a disruption in the physiological location of the BTB. This disorientation of the regulating proteins in the BTB interferes with the process of spermatogenesis. In conclusion, our study demonstrates that exposure to radio frequency modulated EMFs has significant effects on the blood testis barrier, particularly in the composition of proteins in the testes of rodents. The findings suggest that 1 week and 10 weeks of EMF radiation exposure induce disruption in the testicular tissue, leading to a disruption in the physiological location of the BTB. This disruption of the regulating proteins in the BTB hinders the spermatogenesis process.

 Keywords: Electromagnetic Fields (EMFs); Cell Phones; Testes; Blood Testis Barrier (BTB); N-cadherin; Spermatogenesis

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Citation

Citation: Nazli Ece Gungor-Ordueri., et al. “Impact of Electromagnetic Field Radiation on Blood-Testis Barrier Regulatory Protein Levels in Rat Testes”.Acta Scientific Medical Sciences 8.8 (2024): 48-58.

Copyright

Copyright: © 2024 Nazli Ece Gungor-Ordueri., et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.