Acta Scientific Medical Sciences (ASMS)(ISSN: 2582-0931)

Research Article Volume 8 Issue 3

A Simple, Time Efficient Purification Methodology Providing Good Quality Human Pancreatic Islets in the Indian Population

Nithyakalyani Mohan1*, Anusha Sunder2 and Kandappa Himakar Reddy3

1Transplant and Research, Apollo Hospitals, Chennai, Tamilnadu, India
2Research Associate, Apollo Hospital and Research Foundation, Chennai, Tamilnadu, India
3Molecular Biologist Associate, Siruseri Biotech Park for Women, Chennai, Tamilnadu, India

*Corresponding Author: Nithyakalyani Mohan, Senior Scientist, Transplant and Research, Apollo Hospitals, Chennai, Tamil Nadu, India.

Received: February 19, 2024; Published: February 29, 2024

Abstract

Recent advances in islet cell transplantation have led to insulin independence in a majority of islet transplant recipients. Numerous protocols have been published describing isolation of human pancreatic islets, but often rigorous and systematic assessment of their integrity is lacking.

We have described a simple, effective method for the isolation and purification of human pancreatic islets from the Indian population so that we can facilitate preclinical allogeneic islet transplantation studies in the Indian population. In this study, we propose 2 different patterns for purification via a simple filtration process and also via gradient technology for the optimal generation of islets. We have also assessed the functionality of the insulin producing islet cells purified by both the methods. The technique yields sufficient numbers of pure and viable islets to support preclinical research to develop improved strategies to prevent the immune destruction of the transplanted islet graft.

 Keywords: Diabetes; Beta Cells; Islets; Insulin; Human Pancreas; Purification; Ficoll; Filtration

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Citation

Citation: Nithyakalyani Mohan., et al. “A Simple, Time Efficient Purification Methodology Providing Good Quality Human Pancreatic Islets in the Indian Population”.Acta Scientific Medical Sciences 8.3 (2024): 161-170.

Copyright

Copyright: © 2024 Nithyakalyani Mohan., et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.




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