Acta Scientific Medical Sciences (ASMS)(ISSN: 2582-0931)

Research Article Volume 7 Issue 5

Experimental Study of Surgical Wounds in Animals Healing Using a Neodymium Laser

Evsyukova ZA*1, Prazdnikov EN1, Aleksandrushkina NA2,3, Makarevich PI2,3, Popov VS2,3 and Pecherskaya MS4

1Department of Operative Surgery and Topographic Anatomy, MSMDU n.a. Evdokimov, Russia
2Institute for Regenerative Medicine, Lomonosov Moscow State University, Moscow, Russia
3Faculty of Medicine, Lomonosov Moscow State University, Moscow, Russia
4Main Military Clinical Hospital n.a. N.Burdenko, Russia

*Corresponding Author: Evsyukova ZA, Department of Operative Surgery and Topographic Anatomy, MSMDU n.a. Evdokimov, Russia.

Received: March 13, 2023; Published: April 29, 2023

Abstract

The purpose of the current laboratory study was to study the effect of low-intensity energy of a neodymium laser with a wavelength of 1064 nm and a short pulse (650 μs) on the wound process in mice. For the two experiments, we took 33 mice of the C57bl/6 line and 50 mice of the FVB/d20nt line of their own breeding at the age of 12-20 days. The animals were divided into groups, the control group was general, and other groups were formed on the basis of the laser exposure obtained. Mice were removed from the experiment simultaneously by euthanasia (cervical dislocation), after which the material (back skin) was taken and the sections were stained with hematoxylin-eosin and sent for morphological analysis.

After IHC analysis, it should be considered that the neodymium laser used in the presented laboratory study contributes to the acceleration of the onset of the proliferation phase. In the process of this study, it was revealed that migration of macrophages, mast cells and fibroblasts into the wound occurs during LLLT.

During the main stage of the experiment, it was found that for the study of scar formation, the mouse is not the most optimal object, since wounds in mice heal quickly enough without any effects, respectively, it is impossible to assess the effect of the laser on the entire wound process and, especially, the phase of scar remodeling. LLLT should be used from the moment of wounding and laser treatment should be carried out for 48 hours, after which the animals should be removed from the experiment for histological examination; and histological sections of all groups of animals (LLLT 2 and control group with excretion after 24 and 48 hours) should be followed and markers of angiogenesis should be determined, as well as markers of angiogenesis, as well as markers of angiogenesis. It is necessary to conduct a study with the participation of a biophysicist to study the interaction of neodymium laser energy with blood and plasma proteins.

 Keywords: Neodymium Laser; Plasma; Laser Energy

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Citation

Citation: Evsyukova ZA., et al. “Experimental Study of Surgical Wounds in Animals Healing Using a Neodymium Laser”.Acta Scientific Medical Sciences 7.5 (2023): 217-234.

Copyright

Copyright: © 2023 Evsyukova ZA., et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.




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Impact Factor1.403

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