Anapaulla Pereira e Oliveira¹, Ana Flávia Ribeiro da Silva², Isabela Gonçalves da Silva³, Yago de Sousa Castro³, Samella Crystina Silva³, Rafaela Costa Oliveira³ and Sérgio Eustáquio Lemos da Silva³*

¹Lutheran University of Brazil, ULBRA, Brazil
²Universidade Presidente Antõnio Carlos, UNIPAC, Brazil
³Centro Universitário do Triângulo, UNITRI, Brazil

*Corresponding Author: Sérgio Eustáquio Lemos da Silva, Centro Universitário do Triângulo, UNITRI, Brazil.

Received: April 12, 2023; Published: April 21, 2023

Abstract

Escherichia coli is seen as an important bacterium that causes diarrhea in humans and animals. In addition to being associated with intestinal infections, it is commonly associated with urinary tract infections. As a treatment, several antibiotics are used to control these infections, but over time the development of microorganisms resistant to their active principles has been observed, causing medicine to seek new treatment alternatives. Among the most widely used is herbal medicines, compounds made from the extract of medicinal plants. Punica granatum is an herbal medicine well known for its anti-inflammatory action and high antimicrobial content. Thus, it is important to investigate whether Punica granatum extract has antimicrobial action on strains of the bacterium Escherichia coli, inhibiting its growth. This study aimed to analyze the inhibition of Punica granatum extract on the growth of an isolate of Escherichia coli from a patient with urinary tract infection. The isolates of Escherichia coli were donated by the Laboratory of Clinical Analysis San Francisco. The method used for the distribution of the extracts in the plates was the diffusion of discs in agar. The experiment was carried out in triplicate. It was observed that there was no inhibition of the growth of the bacterium Escherichia coli in any concentration of the tested extracts. Between the treatments designed and applied, there was a significant difference, the positive control presented efficiency in the inhibition of Escherichia coli, while the negative control did not present antimicrobial activity, as expected. In view of the results obtained, it was concluded that the aqueous extracts of the fruit of Punica granatum did not inhibit the growth of the bacterium Escherichia coli, but there was a reduction of bacterial growth in the treatments with the peel and mesocarp of the fruit, showing that in both there is antimicrobial activity on isolates of Escherichia coli.

 Keywords: Antibiogram; Punica granatum; Inhibition Halos; Uroculture

References

1. Murray P R. “Manual of clinical microbiology”. 7. ed. Washington, ASM Press, (1999).
2. Tortora G J., et al. “Microbiology”. ed. Porto Alegre: Artmed, (2005).
3. Brandino BA., et al. “Prevalence and factors associated with urinary tract infection”. Review News Lab.4 (2007): 166-176.
4. Tavares W. “Antibiotics and Chemotherapy for the clinician”. São Paulo: Atheneu, (2006).
5. Veiga Junior V F and Pinto AC. “Medicinal plants: safe cure?” Quim Nova, (2005).
6. Lorenzi H and Souza HM. “Ornamental plants in Brazil – shrubs, herbaceous and vines”. 3. ed. New Odessa: Plantarum, (2001).
7. Navarro V., et al. “Antimicrobial evolution of some plants used in Mexican traditional medicine for the treatment of infectious diseases”. Journal of Ethnopharmacology (1996).
8. Menezes SMS., et al. “In vitro and in vivo biological activities of Punica granatum (pomegranate)”. Brazilian Journal of Medicine (2008).
9. Negi PS and Jayaprakasha GK. “Antioxidant and antibacterial activities of Punica granatum peel extracts”. Journal of Food Science (2003).
10. CLSI (Clinical and Laboratory Standards Institute). “Performance Standards for Antimicrobial Disk Susceptibility Tests; Approved Standard”. 8^th. ed. Franklin Lakes, NJ: CLSI, (2003).
11. TELES DG and COSTA MM. “Study of the joint antimicrobial action of aqueous extracts of Tansagem (Plantago major l., Plantaginaceae) and Pomegranate (Punica granatum, Punicaceae) and their interference in the action of amoxicillin in vitro”. Brazilian Journal of Medicinal Plants (2014).
12. Lopes HV and Tavares W. “High Urinary Tract Infection of Community and Hospital Origin: Treatment”. Guidelines Project (2004).
13. Sharma A., et al. “Antibacterial activity of medicinal plants against pathogens causing complicated urinary tract infections”. India Journal of Pharmaceutical Sciences (2009).
14. Pompeo A C L., et al. “Urinary tract infection in the elderly”. Guidelines in Urology (2004).
15. Mccarrell E M., et al. “Antimicrobial activities of pomegranate rind extracts: enhancrmrnt by addition of metal salts and vitamin C”. BMC Complement Alternative Medicine (2008).
16. Rabelo JI C. “Infection of the genitourinary tract. Standardization of the rational use of antimicrobials”. Fortaleza: State Department of Health, (2002).
17. Pereira JV., et al. “In vitro antibacterial and non-adherent effect of Punica granatum Linn extract. On microorganisms of the dental biofilm”. Brazilian Journal of Pharmacognosy, Curitiba, (2006).
18. Voravurhukunchai SP., et al. “Efficacy of crude extracts of Thai medicinal plants on antibiotic-resistant Helicobacter pylori strains isolated peptic ulcers [antimicrobial susceptibility In Gram-negative bacteria – II]”. Clinical Microbiology and Infection Supplement (2004).

Citation

Citation: Sérgio Eustáquio Lemos da Silva., et al. “Antibacterial Effect of Herbal Extracts on Escherichia coli Isolates from Urinary Tract Infection”.Acta Scientific Medical Sciences 7.5 (2023): 154-159.

Copyright

Copyright: © 2023 Sérgio Eustáquio Lemos da Silva., et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.