EG Novikova^1,2*, AV Troitsky¹, LA Cherdantseva³, TN Bystrova¹, VG Selyatitskaya¹

¹Federal Research Center for Fundamental and Translational Medicine, Novosibirsk, Russia
²LLC "Regional Medical diagnostic Center", Novosibirsk, Russia
³Novosibirsk Research Institute of Traumatology and Orthopedics named after Y. L. Tsivyan of the Ministry of Health of Russia, Novosibirsk, Russia

*Corresponding Author: EG Novikova, Federal Research Center for Fundamental and Translational Medicine, Novosibirsk, Russia.

Received: April 05, 2023; Published: April 12, 2023

Abstract

Rationale for the Study: The relevance of the study is due to a significant increase in the proportion of male fertility.

Objective of the Study: To evaluate the therapeutic efficacy of parenteral administration of a 2% solution of oxidized dextran in an in vivo model imitating impaired spermatogenesis after bacterial and viral infections, including COVID-19.

Materials and Methods: Studies performed on rats of the Wistar line. Spermatogenesis disorders were caused by intraperitoneal administration of the lipopolysaccharide Escherichia coli (LPS). In the experimental group, after 30 minutes after intraperitoneal administration of LPS, a 2% solution of oxidized dextran was administered intraperitoneally. After 3 days, the animals were euthanized with an overdose of ether anesthesia and standard histological sections of the testes were prepared, followed by a morphometric assessment of the results.

Results with Data: Parenteral administration of a 2% solution of oxidized dextran can significantly reduce all morphological manifestations of spermatogenesis disorders caused by the introduction of LPS: numerical density (Nai) of spermatozoa in the lumen of the tubule, early and late spermatids, spermatocytes, spermatogoniums, Sertoli cells, Leydig cells, as well as the numerical density (Nai) of blood vessels the peritubular zone of the testes, the volume density (Vv) of the interstitium between the convoluted seminiferous tubules and the volume density (Vv) of cell infiltration.

Conclusion: Oxidized dextran can be a very promising therapeutic and prophylactic drug for disorders of spermatogenesis caused by bacterial and viral infections, including COVID-19.

Keywords: Oxidized Dextran; Disorders of Spermatogenesis; Male Fertility

References

1. Brunner RJ., et al. “Review of Guidelines for the Evaluation and Treatment of Leukocytospermia in Male Infertility”. The World Journal of Men’s Health2 (2019): 128-137.
2. Adamyan LV., et al. “Influence of COVID-19 and other viral infections on male fertility (literature review)”. Problems of Reproduction6 (2020): 77-82.
3. Poplavskaya YuA. “Narrushion of spermatogenesis of male white rats under the influence of lipopolysaccharides of gram-negative bacteria ( coli and S. marcescens) as a cause of reproductive losses”. Abstract of the dissertation for the degree of Candidate of Biological Sciences. Minsk (2015).
4. Wanpeng Yu., et al. “Estrogen promotes Leydig cell engulfment by macrophages in male infertility”. Journal of Clinical Investigation 6 (2014): 2709-2721.
5. Wang M., et al. “Characterization of the Micro-Environment of the Testis that Shapes the Phenotype and Function of Testicular Macrophages”. The Journal of Immunology 198 (2017): 4327-4340.
6. De Falco., et al. “Macrophages Contribute to the Spermatogonial Niche in the Adult Testis”. Cell Reports 12 (2015): 1107-1119.
7. Kalashnikova SA and Polyakova LV. “And the use of bacterial lipopolysaccharide for modeling pathological processes in medical and biological research (literature review)”. Vestnik of New Medical Technologies 2 (2017): 209-219.
8. Nikiforova LR., et al. “Review of preclinical models of sepsis and septic shock”. Laboratory Animals for Scientific Research 4 (2021): 17-28.
9. Patent for the invention of the Russian Federation No. 2728938. "The use of dalargin for the production of means of treatment and prevention of pneumonia". publ. 03.08.2020, Bulb. No. 22.
10. Troitsky AV., et al. “Prospects for the use of oxidized dextran for the prevention of viral pneumonia and pulmonary fibrosis”. Medical Alliance4 (2021): 45-53.

Citation

Citation: EG Novikova., et al. “Experimental Evaluation of the Therapeutic Efficacy of Oxidized Dextran in a Model of Spermatogenesis Disorders in Rats Under the Action of the Endotoxin Escherichia coli”.Acta Scientific Medical Sciences 7.5 (2023): 84-90.

Copyright

Copyright: © 2023 EG Novikova., et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.