Impact of Circulating miRNAs in Cutaneous T-cell Lymphoma Diagnosis: miR-146a and miR-26a as Promising Diagnostic Biomarkers
Marianna Papadaki1, Christina Piperi2, Georgia Damoraki3 and Evangelia Papadavid1*
12nd Department of Dermatology and Venereal Diseases, Attikon General University Hospital, Medical School, National and Kapodistrian University of Athens, Athens, Greece
2Department of Biological Chemistry, Medical School, National and Kapodistrian University of Athens, Athens, Greece
34th Department of Internal Medicine, Attikon General University Hospital, Medical School, National and Kapodistrian University of Athens, Athens, Greece
*Corresponding Author: Evangelia Papadavid, 2nd Department of Dermatology and Venereal Diseases, Attikon General University Hospital, Medical School, National and Kapodistrian University of Athens, Athens, Greece
January 21, 2022; Published: March 14, 2022
Background: The diagnosis of early-stage Mycosis Fungoides (MF) can be very challenging due to overlapping clinicopathologic findings with reactive and inflammatory dermatoses. The aim of this study was to investigate whether selective peripheral blood microRNAs, are involved in CTCL pathogenesis and can be used as potential diagnostic biomarkers to differentiate inflammatory diseases from early MF.
Methods: The expression of miR-148a, miR-338-3p, miR-26a, miR-146a and miR-451 was evaluated in the serum of early MF patients (Ν = 15) using RT-PCR and also measured in skin biopsies (Ν = 10) and CTCL cell lines (My-La and Seax).
Results: Our data showed that miR-26a and miR-146a were significantly upregulated (P < 0.001) in early MF patients compared to controls and could be utilized to differentiate inflammatory skin diseases from early-stage MF. Of importance, miR-451 which has a tumor suppressor role in other hematologic malignancies, was overexpressed in MF.
Conclusions: Altogether these data indicate that liquid biopsy may detect upregulation of specific microRNAs (miR-26a and miR-146a) in early-stage MF and present a reliable non-invasive diagnostic technique. The correlation of liquid biopsy with histological data from the skin biopsy indicates that it may be further used as a supplementary tool to the established criteria for the differential diagnosis of early MF stages from inflammatory skin diseases.
Keywords: CTCL; Diagnosis; miRNA; MF; Biomarkers
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