Acta Scientific Medical Sciences (ASMS)(ISSN: 2582-0931)

Case Report Volume 6 Issue 3

Identification of Rare Bombay Phenotype by Immunohematology Workup: A Case Report

Prem Kumar Essgir1,2* and Nanda Kishore P2

1Assistant Professor, School of Allied Health Sciences, Mallareddy University, Hyderabad, India
2Consultant Transfusion Medicine, Yashoda Super Speciality Hospitals, Somajiguda, Hyderabad, India

*Corresponding Author: Prem Kumar Essgir, Assistant Professor, School of Allied Health Sciences, Mallareddy University, Hyderabad, India.

Received: January 19, 2022; Published: February 11, 2022


Introduction: Rare Bombay phenotype was first reported in Bombay, India by Bhende and Bhatia in 1952. Bombay phenotype is characterized by absence of H, A and B antigens on red cells and in secretions, while plasma contains anti A, anti B and anti H. Genetically Bombay Oh phenotype individuals are termed as homozygous hh/sese.

Methodology: Blood grouping (ABO and RhD) was done in our blood bank by semi-automated Column Agglutination Technology (CAT) as well as Tube agglutination method and performed Adsorption Inhibition Test using saliva (tube method) for confirming secretor status.

Results: A young 21years male voluntary donor donated blood at our blood bank. The ABO Grouping and RhD typing with serum grouping Indicated Bombay Oh RhD Positive group. Further tests done were: Direct Coombs Test/Indirect Coombs Test by 3 cell panel and Adsorption Inhibition Test by using saliva, which confirmed the Bombay blood group.

Conclusion: To identify Bombay Oh Blood group we recommend complete immunohematology workup (Blood Group: Forward and Reverse with Auto control) using standardised agglutination technologies combined with saliva testing for secretary status (with controls).

Keywords: Bombay Blood Group, ABO RhD, Column Agglutination Technology, Saliva Testing, Immunohematology, Direct Coombs Test, Indirect Coombs Test


  1. A B Dutta. “Blood group system. Blood Banking and transfusion”. 1st edition: (2006): 95.
  2. Dr R N Makroo. “ABO blood group system. Compendium of Transfusion medicine”. 2nd edition: Kongposh Publications pvt ltd (2009): 35.
  3. Das S and Kumar HRA. “Bombay Blood A Rare Entity”. Journal of Clinical Biomedical Science3 (2011): 122-125.
  4. Oriol R., et al. “Molecular genetics of H”. Vox Sanguinis 78 (2000): 105-108.
  5. Scharberg EA., et al. “The H blood group system”. Immunohematology3 (2016): 112-211.


Citation: Prem Kumar Essgir and Nanda Kishore P. “Identification of Rare Bombay Phenotype by Immunohematology Workup: A Case Report”.Acta Scientific Medical Sciences 6.3 (2022): 05-07.


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