Naziya Salim1, Baijayanti Mishra2 and Deepa Revi3*
1Assistant Professor, Medical Trust Institute of Medical Sciences, Ernakulam, India
2Professor, St Johns Medical College, Bangalore, India
3Associate Professor, Medical Trust Institute of Medical Sciences, Ernakulam, India
*Corresponding Author: Deepa Revi, Associate Professor, Medical Trust Institute of Medical Sciences, Ernakulam, India.
Received: December 07, 2021; Published: January 24, 2022
Staphylococcus aureus is a common cause of nosocomial infection and biofilm is one of its important virulence factors. Biofilm is a group of microorganisms enclosed in a self-produced exopolysaccharide matrix. S. aureus biofilms have been extensively involved in several chronic untreatable and medical implants related infections. The treatment of biofilm infections is laborious and challenging. On this account, the study was undertaken to detect the biofilm production capacity and antibiotic susceptibility pattern in Staphylococcus aureus derived from various clinical samples. A total 100 S. aureus strains were isolated from various clinical samples and tested for production of biofilm using three methods namely Congo Red Agar Plate method (CRA), Microtiter Plate Method (MTP) and Tube Method (TM). Antibiotic susceptibility comparison between biofilm producers and non-producers were also performed by modified Kirby Bauer’s disc diffusion method. The results showed better correlation between MTP and TM when compared to CRA method. Of the 100 S. aureus strains 6% were strong biofilm producers, 11% were moderate, 47% were weak positive, and 36% were negative by MTP while 56% were strong positive, 26% were moderate positive and 18% were negative for biofilm by TM. The CRA method showed that only 8% were strong positive, 3% were moderate and rest all were negative for biofilm production. None of the S. aureus isolates was resistant to glycopeptides (Vancomycin). The resistance exhibited by S. aureus to penicillin, ciprofloxacin and erythromycin resistant was 100%, 70% and 62% respectively. The antibiotic resistant rate (biofilm producers v/s non producers) for penicillin (64% v/s 36%), erythromycin (38% v/s 24%), and ciprofloxacin (48% v/s 22%) revealed that biofilm producers were more resistant to all the tested antibiotics.
Keywords: Staphylococcus aureus; Biofilm; Microtiter Plate Method; Tube Method; Congo Red Agar Plate Method; Antibiotic Susceptibility Testing
Citation: Deepa Revi., et al. “Evaluation of Biofilm Production and Antibiotic Resistance Pattern Between Biofilm Producing and Non-biofilm Producing Isolates of Staphylococcus aureus”.Acta Scientific Medical Sciences 6.3 (2022): 147-152.
Copyright: © 2022 Deepa Revi., et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.