Acta Scientific Medical Sciences (ASMS)(ISSN: 2582-0931)

Research Article Volume 5 Issue 1

Role of Flaxseed Oil Against Lead Toxicity in Liver of Male Rats

Aljedaani HM1*, Shaikh Omar AKM1 and Elnaggar MHR1,2

1Department of Biological Sciences, Faculty of Sciences, King AbdulAziz University, Jeddah, KSA
2Department of Zoology, Faculty of Sciences, Suez Canal University, Ismailia, Egypt

*Corresponding Author: Aljedaani HM, Department of Biological Sciences, Faculty of Sciences, King AbdulAziz University, Jeddah, KSA

Received: December 05, 2020; Published: December 29, 2020

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Abstract

  The present study aimed to prove the protective role of flaxseed oil (FSO) against lead (Pb) in male rats by measuring the enzyme activities as well as the concentration of some parameters and the histological changes in liver tissues. Forty adult male rats weighing 150-200 g were divided into four groups, control group received distilled water, group two orally administered 60 mg/kg b.w. lead acetate by using the stomach tube day after day, group three orally administered 600 mg/kg b.w. FSO using stomach tube day after day, group four orally administered 600 mg/kg b.w. FSO then 60 mg/kg b.w. lead acetate day after day for four weeks. The results showed an increase in the activity of ALT, AST, ALP and GGT enzymes in group administered lead acetate compared with control group, and convergence in results was observed in the group administered FSO compared with control group. While, in the group administered both FSO and lead acetate, the levels of these enzymes decreased compared to Group II, which was administered lead acetate only. Rats administered lead acetate showed an increase in the values of TBIL, TP, GLU, and ALB. It was observed that there was a convergence in all their values in the group administered FSO compared with control group. While these values were decreased in the group administered both FSO and lead acetate compared to Group II administered lead acetate only. The results of the histological examination, after administration of lead acetate, changes were appeared as swollen, necrotic and apoptotic hepatocytes. Also, many of the portal areas were completely masked by accumulation of inflammatory mononuclear cells and bile ducts were difficult to be seen. Administration of flaxseed oil before lead acetate in the 3rd group showed a significant enhance in the histopathology of the liver cells. The results showed an effect of lead on biochemical measurements, as well as pathological effects on liver tissues in rats. On the contrary, the results showed the protective role of flaxseed oil in maintaining the biochemical parameters and histological tests with their levels and status in the control group. These results confirmed the effective role of FSO against lead and by raising the efficiency of the body to protect or treat it against toxins that affect the important organ of the body the liver.

Keywords: Flaxseed Oil; Lead Acetate; Physiology; Histology; Liver; Rats

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References

  1. Abdelhamid FM., et al. “Ameliorative effect of curcumin against lead acetate–induced hemato-biochemical alterations, hepatotoxicity, and testicular oxidative damage in rats”. Environmental Science and Pollution Research 27 (2020): 10950-10965.
  2. Abdel-Moneim AE., et al. “The potential role of flaxseed oil on lead acetate induced kidney injure in adult male Albino rats”. African Journal of Biotechnology8 (2011a): 1436-1441.
  3. Abdel-Moneim AE., et al. “The redox status in rats treated with flaxseed oil and lead-induced hepatotoxicity”. Biological Trace Element Research1 (2011b): 457-467.
  4. Abdel-Moneim AM., et al. “Curcumin ameliorates lead (Pb2+)-induced hemato-biochemical alterations and renal oxidative damage in a rat model”. Biological Trace Element Research1 (2015): 206-220.
  5. Abdou HM and Hassan MA. “Protective Role of Omega-3 Polyunsaturated Fatty Acid against Lead Acetate-Induced Toxicity in Liver and Kidney of Female Rats”. BioMed Research International (2014): 11.
  6. Alarifi S A., et al. “Effect of corn oil, flaxseed oil and black seed oil on lead acetate-induced hepatic tissue damage: A histological study”. Journal of Medicinal Plant Research 24 (2012): 4128-4134.
  7. Ali SA., et al. “Toxic effects of lead-induced Damage in liver of rats male and the role of ethanolic ginseng extract as protective agents (histological and physiological)”. Merit Research Journal of Medicine and Medical Sciences 9 (2018): 307-315.
  8. Al-Massari MH., et al. “Effect of Nigella sativa oil on female rats exposed to lead”. Acta Scientific Medical Sciences 2 (2020): 1-8.
  9. Azab AE. “Hepatoprotective Effect of Sesame Oil against Lead Induced Liver Damage in Albino Mice: Histological and Biochemical Studies”. American Journal of BioScience 2 (2014): 1-11.
  10. Bharali M. “Effect of acute lead acetate exposure on liver of mice”. Journal of Global Bioscience 2 (2013): 121-125.
  11. Candellone A., et al. “Effect of linseed diet and hazelnut skin supplementation on oxidative status and blood serum metabolites in rabbits”. Progress in Nutrition3 (2019).
  12. Chen C., et al. “Protective Effects of Salidroside on Lead Acetate-induced Oxidative Stress and Hepatotoxicity in Sprague-Dawley Rats”. Biological Trace Element Research (2019): 1-9.
  13. Doumas BT., et al. “Standardization in bilirubin assays: evaluation of selected methods and stability of bilirubin solutions”. Clinical Chemistry9 (1973): 984-993.
  14. Douma BT., et al. “Albumin standards and the measurement of serum albumin with bromcresol green”. Clinica Chimica Acta1 (1997): 21-30.
  15. Ekebas G., et al. “Effect of Flax Seed Oil on Acute Carbon Tetrachloride-induced Hepatic Injury and Determination of Hepatic Apoptosis in Rats”. Journal of Applied Life Sciences International (2019): 1-10.
  16. El Shater AA., et al. “Physiological Studies of the Effect of Moringa olifera and Vitamin (C) on Hepatotoxicity and Oxidative Stress Induced by Lead Acetate in Male Albino Rats. Egypt”. Academic Journal of Biological Sciences 1 (2019): 93-104.
  17. Ganorkar P and Jain R. “Flaxseed--a nutritional punch”. International Food Research Journal 2 (2013): 519-525.
  18. Hegazy A and Fouad U. “Evaluation of Lead Hepatotoxicity; Histological, Histochemical and Ultrastructural Study”. Forensic Medicine and Anatomy Research 3 (2014): 70-79.
  19. Hou G., et al. “Protective effects of a Lachnum polysaccharide against liver and kidney injury induced by lead exposure in mice”. International Journal of Biological Macromolecules 124 (2019): 716-723.
  20. Hussein SA., et al. “Evaluation of the protective role of flaxseed oil on inflammatory mediators, antioxidant defense system and oxidative stress of liver tissue in hypercholesterolemic rats”. International Journal of Pharmacy and Pharmaceutical Sciences 3 (2016): 1480-1489.
  21. McComb R B and Bowers G N. “Study of optimum buffer conditions for measuring alkaline phosphatase activity in human serum”. Clinical Chemistry2 (1972): 97-104.
  22. Mohamed NA., et al. “Flaxseed oil ameliorates methotrexate-induced oxidative stress and hepto-renal toxicity in male rats”. International Journal of Pharmaceutical Sciences And Research3 (2019): 1101-1114.
  23. Mudgal V., et al. “Effect of toxic metals on human health”. The Open Nutraceuticals Journal1 (2010): 94-99.
  24. Osman NN. “The role of antioxidant properties of Celery against lead acetate induced hepatotoxicity and oxidative stress in irradiated rats”. Arab Journal of Nuclear Sciences and Applications 1 (2013): 339-346.
  25. Ozkaya A., et al. “Role of geraniol against lead acetate-mediated hepatic damage and their interaction with liver carboxylesterase activity in rats”. Archives of Physiology and Biochemistry1 (2018): 80-87.
  26. Pagliara P., et al. “Lead nitrate and gadolinium chloride administration modify hepatocyte cell surfaces”. Cell and Tissue Research1 (2003): 41-48.
  27. Peters T. “Total protein: direct Biuret method”. Clinical Chemistry 14 (1968): 1147-1159.
  28. Reitman S and Frankel S. “A colorimetric method for the determination of serum glutamic oxalacetic and glutamic pyruvic transaminases”. American Journal of Clinical Pathology 1 (1957): 56-63.
  29. Rizwan S., et al. “Dietary flaxseed oil supplementation mitigates the effect of lead on the enzymes of carbohydrate metabolism, brush border membrane, and oxidative stress in rat kidney tissues”. Biological Trace Element Research 153 (2013): 279-290.
  30. Rubilar M., et al. “Flaxseed as a source of functional ingredients”. Journal of Soil Science and Plant Nutrition 3 (2010): 373-377.
  31. Shalan MG., et al. “Amelioration of lead toxicity on rat liver with Vitamin C and silymarin supplements”. Toxicology1 (2005): 1-15.
  32. Szasz G. “A kinetic photometric method for serum gammaglutamyl transpeptidase”. Clinical Chemistry 22 (1969): 124-136.
  33. Trinder P. “Determination of glucose in blood using glucose oxidase with an alternative oxygen receptor”. Annals of Clinical Biochemistry 6 (1969): 24-27.
  34. Wang L., et al. “Flaxseed oil improves liver injury and inhibits necroptotic and inflammatory signaling pathways following lipopolysaccharide challene in a piglet model”. Journal of Functional Foods 46 (2018): a 482-489.
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Citation

Citation: Aljedaani HM., et al. “Role of Flaxseed Oil Against Lead Toxicity in Liver of Male Rats”.Acta Scientific Medical Sciences 5.1 (2021): 84-96.




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