Pratibha M* and Abirami B
Department of Biotechnology, Indian Institute of Technology Hyderabad, India
*Corresponding Author: Pratibha M and Abirami B Department of Biotechnology, Indian Institute of Technology Hyderabad, India. email@example.com
Received: August 11, 2020; Published: August 31, 2020
Cell culture techniques help us to understand the cell biology in a much better way. Transformed cell culturing will be one of the useful methods to understand the cancer cell physiology and morphology but culturing of transformed cells in-vitro is a challenging task. The cellular outcome read-outs such as cell signals, cellular secretions, gene expression and downregulation, morphology, etc., vary depending upon the microenvironment given to the cells. Therefore, it is essential to culture the cells in an environment similar to in- vivo for understanding the cell biology behind various mechanisms. This mini review aims at summarizing the conventional and advancements in culturing the transformed cells. Different techniques are highly exploited to study transformed cells, with the most commonly used 3-D semi-solid media culturing method. It is a selective media with suitable substrate that supports the growth of anchorage-independent transformed cells and is useful to elucidate results from products of each cell differentially localized in the media. Early research using soft agar helped to understand and characterize the transformed cells whereas advancements in the semi-solid media helps to screen a wide range of anti-cancer drugs, to study gene regulation, and in biosensors. The major setback of this technique remains a hindrance to extract viable cells from the media. There by to overcome the drawback of conventional semi-solid media technique, over the years, various components are incorporated and substituted to provide a within reach in-vivo conditions. More recently, using semi-solid matrix/scaffolds like matrigel/laminin/alginate, co-culturing of other cells with transformed cells provides the in vivo like niche.
Keywords: Transformed Cell Culture; Semi – Solid Media; Soft Agar; Laminin; Matrigel
Citation: Pratibha M and Abirami B. “Mini Review - Semi-solid Media for Transformed Cell Culturing". Acta Scientific Medical Sciences 4.9 (2020): 145-151.
Copyright: © 2020 Pratibha M and Abirami B. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.