Abstract

The class B vitamins are involved in numerous essential metabolic pathways, it is critical to study the effects of these vitamins at length. In this study, the use of Human Foreskin Fibroblast (HFF) was used to optimize the Vitamin B concentration to be used later in Clone 9 (C9), liver epithelial cell line. The initial cell adhesion and cell proliferation percentages were measured at 5 ug/mL, 10 ug/mL, and 20 ug/mL of synthetic and over the counter (OTC) formulations of Vitamins B6 and B12. In general, HFF-1 and Clone 9 cells displayed favorable rates of proliferation when treated with varying concentrations of Vitamin B6 and Vitamin B12 and the combination of these with concentrations of 5 ug/mL, 10 ug/mL and 20 ug/mL. When administered separately, synthetic Vitamin B12 increased C9 cell proliferation more dramatically than synthetic Vitamin B6. The data suggests a synergistic effect increases cell proliferation the most. This is evident in combined synthetic vitamins B6 and B12 (10,000% increase at 20 ug/mL), but also present in the combined OTC vitamins B6 and B12 (6000% increase at 20 ug/mL). C9 cell morphology appears consistent in all the conditions except for the presence of dark pigmentation in the cells treated with combined B vitamins. The liver is an important organ in the body and performs a fundamental role in the regulation of metabolism, secretion, storage and detoxification. In conclusion, hepatic diseases are areas of concern, thus, it is necessary to identify substances for the treatment of liver diseases, protection of the liver and regeneration of hepatic cells.

Keywords: HFF-1; Human Foreskin Fibroblasts; Clone 9; Rat Hepatocytes; Synergy; Proliferation; Regeneration

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Citation

Citation: Tawil Bill., et al. “The Effect of Over the Counter and Synthetic Vitamin B6 (Pyridoxine) and Vitamin B12 (Cyanocobalamin) on the Behavior of Liver and Non-Liver Cells". Acta Scientific Medical Sciences 4.9 (2020): 46-59.

Copyright

Copyright: © 2020 Tawil Bill., et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.