Acta Scientific Medical Sciences (ISSN: 2582-0931)

Research Article Volume 4 Issue 5

Survey on Molecular Marker and Diagnosis of Helicobacter pylori

Bassam Oudh Al johny1, Abdulaziz Radhi S ALjohni1,2 and Mohamed Morsi M Ahmed1,3

1Department of Biological Science, Faculty of Science, King Abdulaziz University, P O Box 80203, Jeddah 21589, Kingdom of Saudi Arabia
2Department of Laboratory at King Fahad Hospital, Medina, Kingdom of Saudi Arabia
3Nucleic Acids Research Department, Genetic Engineering, and Biotechnology Research Institute (GEBRI), Mubarak City for Scientific Research and Technology Applications, Alexandria, Egypt

*Corresponding Author: Department of Biological Science, Faculty of Science, King Abdulaziz University, P O Box 80203, Jeddah 21589, Kingdom of Saudi Arabia.

Received: March 03, 2020; Published: April 23, 2020

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Abstract

  This paper will discuss Helicobacter pylori which is a bacterium start in human epithelial cells of the gastrointestinal tract. Its infection is related to many different diseases, such as chronic gastritis, peptic ulcers, gastric lymphoma and adenocarcinoma. The infection by Helicobacter pylori is found more than 50% of the world. It is found deep in epithelial cells that hide mucus in the human stomach, where neutral Helicobacter pylori are prevalent. This bacterium is reported as the leading cause of various gastrointestinal illnesses, chronic gastritis, excretory ulcer, abdominal cancer and gastric mucosa related lymphoid tissue (MALT) lymphoma. In this study for the improvement of two PCR-based techniques for detecting Helical kinds of Helicobacter pylori. Molecular diagnosis of Helicobacter pylori strain by using 16S rDNA PCR amplification and direct examination.

Keywords: Molecular Marker; PCR; Gene Diagnosis; Helicobacter pylori

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References

  1. Fox JG., et al.Helicobacter bilis nov., a Novel Helicobacter Species Isolated from Bile, Livers, and Intestines of Aged, Inbred Mice”. Journal of Clinical Microbiology (1995): 445-454.
  2. Smuts HEM and Lastovica AJ. “Molecular Characterization of the 16S rRNA Gene of Helicobacter fennelliae Isolated from Stools and Blood Cultures from Paediatric Patients in South Africa”. Journal of Pathogens (2011): 217376.
  3. Mona Mamoun., et al. “Molecular Identification 0f 16s Ribosomal RNA Gene of Helicobacter pylori Isolated from Gastric Biopsies in Sudan”. American Journal of Microbiological Research2 (2015): 50-54.
  4. Traci L Testerman., et al. “Beyond the stomach: An updated view of Helicobacter pylori pathogenesis, diagnosis, and treatment”. World Journal of Gastroenterology 36 (2014): 12781-12808.
  5. Ashtari S., et al. “The prevalence of Helicobacter pylori is decreasing in Iranian patients”. Gastroenterology and Hepatology 8 (2015): S23-S29.
  6. Brown LM. “Helicobacter pylori: epidemiology and routes of transmission”. Epidemiology 200022 (2000): 283-297.
  7. Bui D., et al. “Serologic Evidence for Fecal-Oral Transmission of Helicobacter pylori”. The American Journal of Tropical Medicine and Hygiene 94 (2016): 82-88.
  8. Covacci A., et al.Helicobacter pylori virulence and genetic geography”. Science 284 (1999): 1328-1333.
  9. Dunn BE., et al.Helicobacter pylori”. Clinical Microbiology Reviews 10 (1997): 720-741.
  10. Everhart JE. “Recent developments in the epidemiology of Helicobacter pylori”. Gastroenterology Clinics of North America 29 (2000): 559-579.
  11. Falush D., et al. “Traces of human migrations in Helicobacter pylori populations”. Science 299 (2003):1582-1585.
  12. Garner JA and TL C: “Analysis of genetic diversity in cytotoxin-producing and non-cytotoxin-producing Helicobacter pylori strains”. The Journal of Infectious Diseases 172 (1995): 290-293.
  13. Lacy BE and Rosemore J. “Helicobacter pylori: ulcers and more: the beginning of an era”. Journal of Nutrition 131 (2001): 2789S-93S.
  14. Lim SH., et al. “Prevalence and risk factors of Helicobacter pylori infection in Korea: a nationwide multicenter study over 13 years”. BMC Gastroenterology 13 (2013): 104.
  15. Logan RP. “Adherence of Helicobacter pylori”. Alimentary Pharmacology and Therapeutics 1 (1996): 3-15.
  16. Makola D., et al.Helicobacter pylori infection and related gastrointestinal diseases”. Journal of Clinical Gastroenterology 41 (2007): 548-548.
  17. Mégraud F. “Transmission of Helicobacter pylori: fecal-oral versus oral-oral route”. Alimentary Pharmacology and Therapeutics 2 (1995): 85-91.
  18. Midolo P and Marshall BJ. “Accurate diagnosis of Helicobacter pylori: Urease tests”. Gastroenterology Clinics of North America Journal 29 (2000): 871-878.
  19. Peleteiro B., et al. “Prevalence of Helicobacter pylori infection worldwide: a systematic review of studies with national coverage”. Digestive Diseases and Sciences 59 (2014): 1698-1709.
  20. Perry S., et al. “Gastroenteritis and transmission of Helicobacter pylori infection in households”. Emerging Infectious Diseases 12 (2006): 1701-1708.
  21. Suerbaum S., et al. “Free recombination within Helicobacter pylori”. Proceedings of the National Academy of Sciences of the United States of America 95 (1998): 12619-12624.
  22. Taylor JM., et al.Helicobacter pylori lipopolysaccharide promotes a Th1 type immune response in immunized mice”. Vaccine23 (2006): 4987-4994.
  23. Woodward M., et al. “An investigation into factors associated with Helicobacter pylori infection”. Journal of Clinical Epidemiology 53 (2000):175-181.
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Citation

Citation: Bassam Oudh Al Johny., et al. “Survey on Molecular Marker and Diagnosis of Helicobacter pylori". Acta Scientific Medical Sciences 4.5 (2020): 50-53.



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