Acta Scientific Medical Sciences (ISSN: 2582-0931)

Research Article Volume 4 Issue 2

Effect of Nigella sativa Oil on Female Rats Exposed to Lead

Majdah H Al-Massari*, Isam M Abu Zeid, Ihsan Ullah and Naser A Alkenani

Department of Biological Sciences, Faculty of Sciences, King Abdulaziz University, Saudi Arabia

*Corresponding Author: Majdah H Al-Massari, Department of Biological Sciences, Faculty of Sciences, King Abdulaziz University, Saudi Arabia.

Received: January 24, 2020; Published: February 05, 2020

×

Abstract

  Environmental pollution is among the major issues the world is facing today. Lead (Pb) contamination even, at a small amount may cause serious damage to vital organs in the body such as liver. On the other hand, medicinal plants have been used for many centuries to cure diseases, especially the black seed (Nigella sativa) (NS). Nigella sativa oil (NSO) is a great source of antioxidants and fatty acids. The objective of this research is to assess the potential use of NSO as the possible protective agent in female rats exposed to Pb using several biochemical parameters, besides the histological changes induced by Pb in liver. In this study, 40 female rats were equally allocated into 4 groups. Rats of the first group (G1) were served as normal controls. Rats of the second group (G2) were orally ingested every two days with a single dose of Pb acetate (150mg/kg/body weight) for a period of 6 weeks. Rats of the third group were exposed to Pb acetate similar to group 2 and Nigella sativa oil ((700mg/kg/day). Rats of the fourth group were administered Nigella sativa oil. Results showed that oral administration of Pb acetate exhibited highly significant increase in the levels of liver function enzymes. Histological examinations of liver sections from rats exposed to only Pb acetate showed severe alterations. This study showed that Nigella sativa oil attenuated the biochemical and histological changes induced by Pb intoxication.

Keywords: Environmental Pollution; Heavy Metals; Pb; Toxicity; Nigella sativa; Nigella Sativa Oil

×

References

  1. Nagle JC., et al. “The Idea of Pollution”. UC Davis Law Review 1-78.
  2. Oke SA. “On the environmental pollution problem: A review”. Journal of Environmental Engineering and Landscape Management 12.3 (2004): 108-113.
  3. Jariwala HJ., et al. “Noise Pollution and Human Health: A Review”. Noise and Air Pollution 3 (2017): 1-4.
  4. Germani AR., et al. Air Pollution and Migration in Italy: An Empirical Investigation at Provincial Level. 14 (2018): 26-31.
  5. Roychoudhury N and Exploration RE. “Soil pollution: Causes, effects and control Soil pollution: Causes, effects and control”. Van Sangyan 3 (2016):1-14.
  6. Mollazadeh H and Hosseinzadeh H. “The protective effect of Nigella sativa against liver injury: A review”. Iranian Journal of Basic Medical Sciences 17.12 (2014): 958-966.
  7. Pourbakhsh H., et al. “Effect of nigella sativa fixed oil on ethanol toxicity in rats”. Iranian Journal of Basic Medical Sciences 17.12 (2014):1020-1031.
  8. Singh R., et al. “Heavy metals and living systems: An overview”. Indian Journal of Pharmacology 43.3 (2011): 246-253.
  9. Tchounwou PB., et al. “Heavy Metals Toxicity and the Environment”. In Molecular, Clinical and Environmental Toxicology (2012): 133-164.
  10. Wani AL., et al. Pb toxicity: a review 8.2 (2015): 55-64.
  11. Narayana K and Al-Bader M. “Ultrastructural and DNA damaging effects of Pb nitrate in the liver”. Experimental and Toxicologic Pathology 63.1-2 (2011): 43-51.
  12. Brochin R., et al. The Cellular Effect of Pb Poisoning and Its Clinical Picture. 5.2 (2008): 34-42. 
  13. Gillis BS., et al. Analysis of Pb toxicity in human cells 13.1 (2012):1-6.
  14. Al-Attar., et al. “Preventive effects of black seed (Nigella sativa) extract on Sprague Dawley rats exposed to diazinon”. Australian Journal of Basic and Applied Sciences 4.5 (2010): 957-968.
  15. Abdelmeguid NE., et al. “Effects of Nigella sativa and thymoquinone on biochemical and subcellular changes in pancreatic β-cells of streptozotocin-induced diabetic rats”. Journal of Diabetes 2.4 (2010): 256-266.
  16. Mohamadin AM., et al. “Protective effects of Nigella sativa oil on propoxur-induced toxicity and oxidative stress in rat brain regions”. Pesticide Biochemistry and Physiology 98.1 (2010): 128-134.
  17. Rahmani AH., et al. “Therapeutic implications of black seed and its constituent thymoquinone in the prevention of cancer through inactivation and activation of molecular pathways”. Evidence-based Complementary and Alternative Medicine (2014).
  18. Salem ML and Hossain MS. “Protective effect of black seed oil from Nigella sativa against murine cytomegalovirus infection”. International Journal of Immunopharmacology 22.9 (2000): 729-740.
  19. Shabana A., et al. “Cardiovascular benefits of black cumin (Nigella sativa)”. Cardiovascular Toxicology 13.1 (2013): 9-21.
  20. Anon. “The cardiovascular actions of the volatile oil of the black seed (Nigella sativa) in rats: elucidation of the mechanism of action”. General Pharmacology: The Vascular System 24.5 (1993): 1123-1131.
  21. Dollah MA., et al. “Toxicity effect of Nigella sativa on the liver function of rats”. Advanced Pharmaceutical Bulletin 3.1 (2013): 97-102.
  22. Bioequivalence MOJ. Nigellalogy: A Review on Nigella Sativa 3.6 (2017).
  23. Asif HM., et al. “Nigella sativa: Monograph”. Journal of Pharmacognosy and Phytochemistry JPP 4.44 (2015): 103-106.
  24. Akloul R., et al. Composition and biological activities of the essential oil of Nigella sativa seeds isolated by accelerated microwave steam distillation with cryogenic grinding 1.3 (2014): 23-33.
  25. Suvarna KS., et al. “Bancroft's Theory and Practice of Histological Techniques”. 7th ed. Oxford, Churchill Livingstone Elsevier (2013): 654.
  26. Dapul H and Laraque D. “Pb Poisoning in Children”. Advances in Pediatrics 61.1 (2014): 313-333.
  27. Randhawa AM and Ahmed Alenazi S. “Neuropsychiatric Effects of Nigella sativa (Black Seed) – A Review”. Alternative and Integrative Medicine 05.01 (2016).
  28. Offor SJ., et al. “Pb induced hepato-renal damage in male albino rats and effects of activated charcoal”. Frontiers in Pharmacology 8 (2017):1-10.
  29. Ahmed MA and Hassanein KMA. Cardio protective effects of Nigella sativa oil on Pb induced cardio toxicity: Anti inflammatory and antioxidant mechanism 4.5 (2013): 72-80.
  30. Radwan RR and Mohamed HA. “Nigella sativa oil modulates the therapeutic efficacy of mesenchymal stem cells against liver injury in irradiated rats”. Journal of Photochemistry and Photobiology B: Biology (2018).
×

Citation

Citation: Majdah H Al-Massari., et al. “Effect of Nigella sativa Oil on Female Rats Exposed to Lead". Acta Scientific Medical Sciences 4.2 (2020): 01-08.



Member In





News and Events


  • Certification for Review
    Acta Scientific certifies the Editors/reviewers for their review done towards the assigned articles of the respective journals.
  • Submission Timeline for Upcoming Issue
    The last date for submission of articles for regular Issues is July 10, 2020.
  • Publication Certificate
    Authors will be issued a "Publication Certificate" as a mark of appreciation for publishing their work.
  • Best Article of the Issue
    The Editors will elect one Best Article after each issue release. The authors of this article will be provided with a certificate of “Best Article of the Issue”.
  • Welcoming Article Submission
    Acta Scientific delightfully welcomes active researchers for submission of articles towards the upcoming issue of respective journals.
  • Contact US