Acta Scientific Medical Sciences (ISSN: 2582-0931)

Research Article Volume 4 Issue 2

Effect of Intestinal Helminths on the Protein Content of Heavy Metals Polluted Tissues of Lethrinus mahsena Fish

Amaal Hassan1,2*, Salim M El-Hamidy1, Najla Al-Saud1, Sabah M Hassan1,3, Shaikh AM1,4, Khalid Mohammed Algamdi1, Hanan Alhelali1 and Habeeb M Al-Solami1

1Biology Department, Science College, King Abdulaziz University, Jeddah, Saudi Arabia
2Zoology Department, Faculty of Science, Sohag University, Sohag, Egypt
3Department of Genetics, Faculty of Agriculture, Ain Shams University, Cairo, Egypt
4Princess, Doctor Najla Bint Saud Al-Saud Distinguished Research Centre for Biotechnology, Jeddah, Saudi Arabia

*Corresponding Author: Amaal Hassan, Associate professor of parasitology, Biology Department, Science College, King Abdulaziz University, Jeddah, Saudi Arabia.

Received: January 22, 2020; Published: January 31, 2020



  Fish are valued as an ideal source of high-quality proteins, which contain large quantities of essential amino acids. The present investigation is designed to study the effect of intestinal helminths in the fish; Single, double and triple helminths infection on the protein content of fish Lethrinus mahsena in the presence of some heavy metals (HMs). In this study, total of 130 emperor fish were collected between November 2015 and May 2016 from the coast of Jeddah. The concentration of the HMs in the infected and uninfected fish was analyzed using the atomic flame absorption spectrometer. Total proteins were extracted from infected and uninfected fish tissues and separated by 12% of SDS-PAGE. The results revealed that individual cestodes and triple infection caused lower concentrations of As, Cd, Cu and Fe in fish tissues. While double lesions have caused a decrease in the concentration of Cd and Cu. There were no significant differences between the protein contents of infected and uninfected fish. This indicates that the presence of intestinal worms in the fortified intestine can reduce the harmful effect of accumulated HMs on the protein content of fish despite HMs contamination.

Keywords: Helminths; Heavy Metals; Lethrinus mahsena; Protein; Infection; Contamination



  1. Rohde K. "Ecology of marine parasites". University of Queensland Press (St. Lucia and New York) (1982): 230.
  2. Habib SA and Abou Shehatta, S “Effect of Heavy Metals Pollution on Protein Biosynthesis in Catfish”. JWRP Jewish Women's Renaissance Project 5 (2013): 555-562. 
  3. Mziray PK and Kimirei IA. “Bioaccumulation of heavy metals in marine fishes (Siganus sutor, lethrinus harak, and Rastrelliger kanagurta) from Dar es Salaam Tanzania”. Regional Studies in Marine Science 7 (2016): 72-80.
  4. Sures B., et al. “Intestinal fish parasites as heavy metal bioindicators: A comparison between Acanthocephalus lucii (Palaeacanthocephala) and the zebra mussel, Dreissena polymorpha”. Bulletin of Environmental Contamination and Toxicology 59 (1997): 14-21. 
  5. Zaghloul K. “Usage of zinc and calcium in inhibiting the toxic Effect of copper on the African catfish, Clarias gariepinus”. Journal of the Egyptian-German Society of Zoology 35 (2001): 99-119.
  6. Sures B., et al. "Parasites as accumulation indicators of heavy metal pollution”. Parasitology Today 15 (1999): 16-21. 
  7. Evans DW., et al. “The effect of digenean (Platyhelminthes) infections on heavy metal concentrations in Littorina littorea”. Journal of the Marine Biological Association of the United Kingdom 81 (2001): 349-350. 
  8. Khan R and Thulin J. “Influence of pollution on parasites of aquatic animals”. Advances in Parasitology 30 (1991): 201-238. 
  9. Hassan A., et al. “Role of parasitic helminths in bioremediating some heavy metal accumulation in the tissues of Lethrinus mahsena”. Turkish Journal of Fisheries and Aquatic Sciences 18 (2018): 435-443. 
  10. Ali AA., et al. “Investigation of Heavy Metals Pollution in Water, Sediment and Fish at Red Sea-Jeddah Coast-KSA at two different locations”. Journal of Applied Environmental and Biological Sciences 1 (2011): 630-637.
  11. Hassan A., et al. “Impact of nematode helminths on metal concentrations in the muscles of koshar fish, Epinephelus summana, in Jeddah, Saudi Arabia”. JOBAZ 74 (2016): 56-61. 
  12. Smith D. “Fish Muscle Proteins: Extraction, Quantitation, and Electrophoresis. In: Nielsen S.S. (eds) Food Analysis Laboratory Manual”. Food Science Texts Series. Springer, Boston, MA (2010).
  13. Laemmli UK. “Cleavage of structural proteins during the assembly of the head of bacteriophage T4”. Nature 227 (1970): 680-685.
  14. Sures B. “Host-parasite interactions from an ecotoxicological perspective”. Parassitologia 49 (2007): 173-176. 
  15. Azmat R., et al. “Natural bioremediation of heavy metals through nematode parasite of fish”. Biotechnology 1 (2008): 139-143.
  16. Eissa IA., et al. “Bioremediation for heavy metals in some Red Sea fishes in Suez, Egypt”. Suez Canal Veterinary Medicine Journal 2 (2012): 341- 356.
  17. Sures B “Environmental parasitology: Relevancy of parasites in monitoring environmental pollution”. Trends in Parasitology 20 (2004):170-177. 
  18. Nachev M., et al. “Comparison of the metal accumulation capacity between the acanthocephalan Pomphorhynchus laevis and larval nematodes of the genus Eustrongylides sp. infecting barbel (Barbus barbus)”. Parasite Vector 6 (2013): 1-8. 
  19. Samuel D and George PV “Mineral constituents of freshwater fish Channa striatus infected with acanthocephalan parasite, Pallisentis nagpurensis”. Indian Journal of Fisheries 47 (2000): 215-224.
  20. Oyoo-Okoth E., et al. “Parasites modify sub- cellular partitioning of metals in the gut of fish”. Aquatic Toxicology 106 (2012): 76-84. 
  21. Guyton AC. “Text book of medical physiology” 2nd edition. Saunders Philadelphia and London. (1961): 141-145.
  22. De Burin AD. “Biochemical toxicology of environmental agents”. Elsevier, Elsevier; Amsterdam, Netherlands (1976): 1554.
  23. Mehdi DS. “Effect of parasites on the biochemical constituents of fishes Liza abu”. M.Sc. Thesis, University of Basrah (1989): 98.
  24. Al-Hadithi IAW., et al. “Effect of intensity of Infection with parasites on protein content in Liza abu (Heckle)”. Basrah Journal of Agricultural Sciences 3 (1990): 209-218. 
  25. Ali AH. “Pathological effects of helminths parasitic on some local fishes”. M.Sc. thesis, College of Agriculture, Basrah University (2001).
  26. Kumar PA. “Biochemical effects on protein and free amino acid metabolism in Catla catla and Labeo rohita due to Pallisentis nagpurensis infection”.‏ AIJRFANS 6 (2014): 82- 85. 
  27. Soliman MF. “Parasitological aspects and biochemical changes of infected cultured tilapia [Oreochromis hybrid”. Acta Ichthyologica Et Piscatoria 34 (2004): 21-32. 
  28. Al-Khayat BH and Al-Salehi MS. “The effect of parasitism on some biochemical compounds in liver of Acanthobrama marmid infected with Pseudochetosoma salmincola (Digenea) and Ligula intestinalis (Tapeworme)”. Rafidain Journal of Science 18 (2007): 30-40.
  29. Abdelhamid AM and El-Ayouty SA “Effect on catfish (Clarias lazera) composition of ingestion rearing water contaminated with lead or aluminum compounds”. Arch Tierernahr 41 (1991): 757-763.


Citation: Amaal Hassan., et al. “Effect of Intestinal Helminths on the Protein Content of Heavy Metals Polluted Tissues of Lethrinus mahsena Fish". Acta Scientific Medical Sciences 4.2 (2020): 215-221.


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