Abstract

Objective: The know the pharmacological effect of Krameria cytisoides extract in vitro and in vivo, as well as the cytotoxicity of this compound, determined.

Material and Methods: For in vitro determination, concentrations of 40, 80 and 160 mg/kg of K. cytisoides were used. Cytotoxicity was assessed with VERO cells using concentrations between 10 and 160 µg/L. The pharmacological effect in vivo was investigated with five groups males per groups 3 of Balb/c mice. All mice were inoculated with 1x10³/ µL trypomastigotes. Three groups were injected with 40, 80 or 160 mg/mL. The fourth group was treated with 100 mg/kg of the benznidazole reference drug, and no treatment was given to the fifth group.

Results: The results demonstrated that the LD₅₀ was 69 µg/mL, with an LC₅₀ cytotoxicity of 160 µg/mL; thus, no toxic effect was observed. Regarding the in vivo results, 61% and 74% flagellates death was observed at the highest concentration of K. cytisoides, and the reference drug benznidazole, respectively.

Discussion and Conclusions: According to the results, the low toxicity and lethality of K. cytisoides compared to benznidazole suggests that the former could be used as a drug of choice for Chagas disease.

Keywords: Krameria Cytisoides; T. Cruzi; Epimastigotes; Trypomastigotes

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Citation

Citation: Jiménez-Cardoso E., et al. "Pharmacological Effect of Krameria cytisoides on Trypanosoma cruzi in vitro and in vivo Krameria cytisoides on Trypanosoma cruzi". Acta Scientific Medical Sciences 4.1 (2020): 141-145.

Copyright

Copyright: © 2020 Jiménez-Cardoso E., et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.