Priyanka Sawant and Jignasha Thumar*
Department of Microbiology and Biotechnology, Gujarat University, Ahmedabad, Gujarat, India
*Corresponding Author: Jignasha Thumar, Department of Microbiology and Biotechnology, Gujarat University, Ahmedabad, Gujarat, India.
Received: January 17, 2023; Published: February 02, 2023
Since enzymes are bioactive chemicals, they are of interest to scientists all over the world. Demand for microbial enzymes is rising rapidly across a wide range of sectors as their importance in ensuring the security, sustainability and efficiency of biotech processes becomes more widely recognised. Media optimization is a powerful tool to enhance production capacity from biological sources (microorganisms). In this present study, samples were collected from mangrove-rich sources such as Borivali Monari Creek and Jhow Island, Maharashtra, western India. Samples collected were mangrove-associated soil, mangrove root, and sea water. The type of sampling was random. Samples were collected with prior permission from the mangrove cell maharashtra government. A total of 30 samples were processed, from which 62 isolates were obtained. These isolates were all screened for industrially important enzymes (protease, amylase, and cellulase), and the best protease producer was selected for further studies. The method of optimization was "ONE VARIABLE AT A TIME" (OVAT) ” and method is a well-known optimization technique. Optimization was carried out for parameters like inoculum size, pH of the growth medium, various carbon sources, varied nitrogen sources, and metal ions and cations. Casein was used as substrate, and casein was prepared in various pH buffers for the enzyme assay. At 5 minutes after incubation of enzyme substrate mixture, enzyme activity was at its peak, and substrate degradation in the medium was minimal as compare 65 minutes set. Enzyme activity decreased d ramatically as substrate pH increased (between 11 and 12 pH). This optimization supports maximizing the yield of protease production and is of great benefit to the biotech industry. The present study shows that with time stability of enzyme reduces and residues of degraded substrate increases within the reaction mixture. Here in our experiment variation in substrate pH were studied, which showed that protease from Bor S17B13 isolate has optimum enzyme activity at substrate pH 10 at highly alkaline pH then 10 pH, activity of enzyme was reduced. A phenomenon called dissociation of substrate (casein) was seen at casein solution 13 pH.
Keywords: Mangrove Ecosystem; Halotolerant; Protease; Bor S17B13; Substrate pH; Enzyme Substrate; ES
Citation: Priyanka Sawant and Jignasha Thumar. “Impact of Substrate pH and Enzyme-Substrate Incubation Time on Protease from a New Halo-tolerant Bor S17B13 Found in the Mangroves of Western India". Acta Scientific Microbiology 6.2 (2023): 03-09.
Copyright: © 2022 Priyanka Sawant and Jignasha Thumar. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.