Acta Scientific Microbiology (ISSN: 2581-3226)

Research Article Volume 4 Issue 11

Anogeissus leiocarpus: A Potential Inhibitor of Some Extended Spectrum β-Lactamase Bacteria

Dahiru M1*, MT Adamu2, Kolawole OS1 and Isyaka MS1,3

1Department of Biological Sciences, Federal University of Kashere, Gombe State, Nigeria
2Department of Microbiology, Faculty of Science, Gombe State University, Nigeria
3Department of Chemical Sciences, Federal University of Kashere, Gombe State, Nigeria

*Corresponding Author: Dahiru M, Department of Biological Sciences, Federal University of Kashere, Gombe State, Nigeria.

Received: September 06, 2021 ; Published: October 26, 2021



The plant of Anogeissus leiocarpusis widely used for medicinal purposes by Kashere people in Gombe State, Nigeria. To provide scientific barking for its utilization, ten different concentrations of ethanolic stem bark extract were prepared and tested on E. coli, K. pneumoniae, P. aeruginosa and S. typhi ESBLs bacteria, using standard procedure and phytochemical components of the plant extract was also determined using GC-MS. The results obtained indicate an antibacterial activity at varying concentrations w/v and zones of inhibition diameter (ZID) ranging from 15.67 ± 0.57 mm to 17.00 ± 1.00 mm with a significance difference (p < 0.05) across bacteria tested. Minimum inhibitory concentration (MIC) were between 66.67 ± 23.1 mg/L to 16.67 ± 5.77 mg/L. Minimum Bactericidal Concentration and Minimum Bacteriostatic Concentration were between 266.67 ± 2.38 mg/L to 106.67 ± 46.19 mg/L and 266.67 ± 92.37 mg/L to 80.00 ± 0.00 mg/L respectively. The MBC/MIC ratio was bacteriostatic for E. coli (0:53) and S. typhi (7:1) while bactericidal for K. pneumonia (4:1) P. aeruginosa (3:1). Twenty five compounds were identified with Myristoleic acid; Z-7-Tetradecenal; 4,5-Dimethyl-4-Hexen-3-one; 1,15-Hexadecadiene and Linoleoyl chloride as most abundant. A more in depth assessment of A. leiocarpus should be carried o isolate bioactive individual compounds responsible for specific antibacterial activity.

Keywords: Bioactive; ESBLs; Bactericidal; Fatty Acid; Herbal



  1. Chaman Lal and Verma LR. “Use of certain bio-products for insect-pest control”. Indian Journal of Traditional Knowledge1 (2006): 79-82.
  2. Sermakkani M and Thangapandian V. “GC-MS Analysis of Cassia italica Leaf Methanol Extract”. Asian Journal of Pharmaceutical and Clinical Research2 (2012): 0974-2441.
  3. Huttner A., et al. “Antimicrobial resistance: A global view from the 2013 World Healthcare-Associated Infections Forum”. Antimicrobial Resistance and Infection Control 31 (2013): 1-13.
  4. World Health Organization. “Antimicrobial resistance: global report on surveillance”. WHO Press, World Health Organization, 20 Avenue Appia (2014): 3-5.
  5. Roca I., et al. “The global threat of antimicrobial resistance: Science for intervention”. New Microbes and New Infections 6 (2015): 22-29.
  6. Piddock L J. “Reflecting on the final report of the O’Neill Review on Antimicrobial Resistance”. Lancet Infectious Disease 16 (2016): 767-768.
  7. Ibrahim MB., et al. “Antibacterial effect of extract of leaf, stem and roof bark of Anogeissus leiocarpus on some bacterial organisms”. Journal of Pharmaceutical Research and Development 1 (1997): 20-23.
  8. Selvakumar S and Sindhuja J. “Determination of Bio Pharmaceutical Components of A Novel Poly Herbal Formulation by GC-MS Analysis”. IAJPR11 (2017): 830-835.
  9. Drieux L., et al. “Phenotypic detection of extended-spectrum β-lactamase production in Enterobacteriaceae: review and bench guide”. Clinical Microbiology and Infection 14 (2008): 90-103.
  10. Adhikari R., et al. “Detection of Methicillin Resistant Staphylococcus aureus and Determination of Minimum Inhibitory Concentration of Vancomycin for Staphylococcus aureus Isolated from Pus/Wound Swab Samples of the Patients Attending a Tertiary Care Hospital in Kathmandu, Nepal”. Canadian Journal of Infectious Diseases and Medical Microbiology (2017): 1-6.
  11. Ramalivhana J N., et al. “Antibacterial activity of honey and medicinal plant extracts against Gram negative bacteria”. 3.4 (2014): 616-625.
  12. Delnavazi M R., et al. “Antioxidant and antibacterial activities of the essential oils and extracts of Dorema ammoniacum roots and aerial parts”. Research Journal of Pharmacognosy (RJP)4 (2014): 11-18.
  13. Performance Standards for Antimicrobial Susceptibility Testing; Twenty-First Informational Supplement: M100-S21 31.1 (2011).
  14. Yusha’u M., et al. “Biological activity and phytochemical constituents of Tamarindus indica stem bark extracts”. Sky Journal of Microbiology Research9 (2014): 067-071.
  15. Saleem U., et al. “In-Vitro Antimicrobial Susceptibility Testing of Leaves Methanol Extract and Latex of Euphorbia Helioscopia Using Agar Well Diffusion and Broth Dilution Methods”. The Journal of Animal and Plant Sciences1 (2015): 261-267.
  16. Devender R and Ramakrishna H. “GC-MS analysis of bioactive compounds in methanolic extract of Leucaslavandula efolia-A potential folklore medicinal plant”. Journal of Pharmacognosy and Phytochemistry 6.4 (2017): 405-406.
  17. Okpekon T. “Antiparasitic activities of medicinal plants used in Ivory Coast”. Journal of Ethnopharmacology1 (2004): 91-97.
  18. Mann A., et al. “Phytochemical and antibacterial screening of Anogeissus leiocarpus against some microorganisms associated with infectious wounds”. African Journal of Microbiology Research 2 (2008): 60-062.
  19. Shuaibu M N. “Castalagin from Anogeissus leiocarpus mediates the killing of Leishmania in vitro”. Parasitology Research6 (2008): 1333-1338.
  20. Ademola I O and Eloff J N. “In Vitro Anthelmintic Effect of Anogeissus leiocarpus (DC.) Guill. and Perr. Leaf Extracts and Fractions on Developmental Stages of Haemonchus Contortus”. African Journal of Traditional, Complementary and Alternative Medicines 2 (2011): 34-43.
  21. Arbab AH. “Review on Anogeissus leiocarpus A Potent African Traditional Drug”. International Journal of Research in Pharmacy and Chemistry 3 (2014): 496-500.
  22. Sani H D and Aliyu BS. “A Survey of Major Ethno Medicinal Plants of Kano North, Nigeria, Their Knowledge and Uses by Traditional Healers. Bayero Journal of Pure and Applied Sciences2 (2011): 28-34.
  23. Edewor T I., et al. “Determination of antibacterial activity, total phenolic, flavonoid and saponin contents in leaves of Anogeissus leiocarpus (DC.) Guill and Perr”. Journal of Coastal Life Medicine4 (2016): 310-314.
  24. Novo D J., et al. “Multiparameter flow cytometric analysis of antibiotic effects on membrane potential, membrane permeability, and bacterial counts of Staphylococcus aureus and Micrococcus luteus”. Antimicrobial Agents and Chemotherapy 44 (2000): 827-834.
  25. Parsons J B., et al. “Membrane Disruption by Antimicrobial Fatty Acids Releases Low-Molecular-Weight Proteins from Staphylococcus aureus”. Journal of Bacteriology19 (2012): 5294-5304.
  26. Bisignano G., et al. “In vitro antibacterial activity of some aliphatic aldehydes from Oleaeuropaea L”. FEMS Microbiology Letters 1 (2001): 9-13.
  27. Lóránd T., et al. “Synthesis and antibacterial study of unsaturated Mannich ketones. European Journal of Medicinal Chemistry9 (2001): 705-717.
  28. Trombetta D., et al. “Study on the mechanisms of the antibacterial action of some plant α,β‐unsaturated aldehydes”. Letters in Applied Microbiology 35 (2002): 285-290.
  29. Szakiel A., et al. “Antibacterial and Antiparasitic Activity of Oleanolic Acid and its Glycosides isolated from Marigold (Calendula officinalis)”. Planta Medica 74 (2008): 1709-1715.
  30. Baden C U and Dobler S. “Potential benefits of iridoid glycoside sequestration in Longitarsus melanocephalus (Coleoptera, Chrysomelidae)”. Basic and Applied Ecology 1 (2009): 27-33.
  31. Wang Q., et al. “Antibacterial effects of two monoterpene glycosides from Hostap lantaginea (lam.) Aschers”. Journal of Food Biochemistry 2 (2016).
  32. Tagousop C N., et al. “Antimicrobial activities of flavonoid glycosides from Graptophyllum grandulosum and their mechanism of antibacterial action”. BMC Complementary and Alternative Medicine 18 (2018): 252.
  33. McGaw LJ., et al. “Antibacterial effects of fatty acids and related compounds from plants”. South African Journal of Botany 68 (2002): 417-423.
  34. Kabara JJ., et al. “Fatty acids and derivatives as antimicrobial agents”. Antimicrobial Agents and Chemotherapy 2 (1972): 23-28.
  35. Cerdeiras MP., et al. “A new antibacterial compound from Ibicella lutea”. Journal of Ethnopharmacology 73 (2000): 521-525.
  36. Dilika F., et al. “Antibacterial activity of linoleic and oleic acids isolated from Helichrysum pedunculatum: a plant used during circumcision rites”. Fitoterapia 71 (2000): 450-452.
  37. Yff B T S., et al. “The pharmacological screening of Pentanisiaprunelloides and the isolation of the antibacterial compound palmitic acid”. Journal of Ethnopharmacology 79 (2002): 101-107.
  38. Lacey RM and Lord V L. “Sensitivity of staphylococci to fatty acids: novel inactivation of linolenic acid by serum”. Journal of Medical Microbiology 14 (1981): 41-49.
  39. McDonald M I., et al. “Antibacterial activity of hydrolysed linseed oil and linolenic acid against methicillin-resistant Staphylococcus aureus”. The Lancet 2 (1981): 1056.
  40. Ohta S., et al. “Antibiotic effect of linolenic acid from Chlorococcum strain HS-101 and Dunaliella primolecta on methicillin-resistant Staphylococcus aureus”. Journal of Applied Phycology 7 (1995): 121-127.
  41. Giamarellos-Bourboulis EJ., et al. “In-vitro inhibitory activity of gamma-linolenic acid on Escherichia coli strains and its influence on their susceptibilities to various antimicrobial agents”. Journal of Antimicrobial Chemotherapy 36 (1995): 327-334.
  42. Peters J S., et al. “Inhibition of photosynthetic electron transport by palmitoleic acid is partially correlated to loss of thylakoid membrane proteins”. Plant Physiology and Biochemistry 41 (2003): 117-124.
  43. Zheng C J., et al. “Fatty acid synthesis is a target for antibacterial activity of unsaturated fatty acids”. FEBS Letter 579 (2005): 5157-5162.
  44. Schönfeld P and Wojtczak L. “Fatty acids as modulators of the cellular production of reactive oxygen species”. Free Radical Biology and Medicine 45 (2008): 231-241.
  45. Kenny J G., et al. “The Staphylococcus aureus response to unsaturated long chain free fatty acids: survival mechanisms and virulence implications”. PLoS One 4 (2009): e4344.
  46. Jenecius A and Mohan VR. “GC-MS Analysis of Bioactive Componentson the Stem Extract of Bacolepis Nervosa (Wight and Arn.) Decne.Ex Moq. (Periplocaceae)”. Word Journal of Pharmacy and Pharmaceutical Sciences4 (2014).


Citation: Dahiru M., et al.Anogeissus leiocarpus: A Potential Inhibitor of Some Extended Spectrum β-Lactamase Bacteria”. Acta Scientific Microbiology 4.11 (2021): 75-81.


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