Abstract

Waste management is a global issue which has become increasingly serious with population and economic growth, particularly in Nigeria. Bioethanol is a suitable substitute to hydrocarbon as it is produced by fermenting carbohydrate biomass derived from plants. This research investigation, the use of cassava peels serves as the main carbon source for production of bioethanol. Bioethanol production from the peels of cassava was examined using co-culture of Aspergillus niger and Zymomonas mobilis through simultaneous saccharification and fermentation process. 0.1 M sulphuric acid and sodium hydroxide was used for substrate pretreatment. Aspergillus niger and Zymomonas mobilis were added to ferment the peels at 28^oC for 5 days during which the total bacterial and fungal count, mineral composition, cellulose content, reducing sugar, pH, temperature and the quantity of bioethanol produced were determined. The bacterial count (3.58 x 10³), fungal count (2.81 x 10³), mineral composition (15.64 mg/100g), cellulose content (66.08% and 69.29%), reducing sugar (6.98 ± 0.01 and 8.98 ± 0.00 g/l), pH (7.67), temperature was at 36^oC at the initial stage of fermentation and later remain static at 28^oC till 5^th day of fermentation, the total quantity of bioethanol yield obtained was (17.08 g/ml) after the 5^th day of fermentation. Fermented hydrolysate was distillated at 78^oC. Therefore, Aspergillus niger and Zymomonas mobilis are proven effective for saccharification and fermentation of cassava peels for bioethanol production as an alternative fuel and energy source.

Keywords: Bioethanol; Cassava Peels; Pretreatment; Fermentation

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Citation

Citation: Deke Victoria Adegunloye and Olawumi Janet Olowe. “Effects of Pretreatments on the Production of Bioethanol from Manihot Esculenta Crantz Peels Using Zymomonas mobilis”. Acta Scientific Microbiology 4.11 (2021): 66-74.

Copyright

Copyright: © 2021 Deke Victoria Adegunloye and Olawumi Janet Olowe. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.