Acta Scientific Microbiology (ISSN: 2581-3226)

Research Article Volume 4 Issue 11

Distribution and Antibiotic Susceptibility Profiles of Vibrio Species Isolated from Selected Abattoirs in Akoko South-West, Ondo State, Nigeria

Osunla Charles Ayodeji*

Department of Microbiology, Adekunle Ajasin University, Ondo State, Nigeria

*Corresponding Author: Osunla Charles Ayodeji, Department of Microbiology, Adekunle Ajasin University, Ondo State, Nigeria.

Received: September 22, 2021; Published: October 14, 2021

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Abstract

Many Vibrio species are pathogenic to humans and have been implicated in food-borne disease. They are naturally found in the estuarine and marine environment. The aim of this study is to study the distribution and antibiotics susceptibility profiles of Vibrio species recovered from two selected abattoirs in Akoko Southwest. A total of fifteen (15) samples consisting of Slab swabs (n = 4), Meat (n = 4), Effluent (n = 4), and Knife (n = 3) were collected from Abattoirs in Akungba and Iwaro-Oka Akoko. Bacteriological investigation was carried out in accordance with the microbiological standard method by serially diluted each sample in test tubes filled with 9ml of enrichment medium (Alkaline Peptone Water) and were isolated through the use of TCBS agar which is one of the recommended media for the selective isolation of Vibrio species. The overall proportion of Vibrio positive isolates was 86.67% in all samples, of which 100% were from effluent and meat, 75% slab and 66.67% knife. It was observed that more Vibrio spp. were isolated from the meat and effluent discharged than from other sources. The bacteria (Vibrio species) isolated were subjected to various biochemical tests and were also subjected to antimicrobial susceptibility tests. The isolates showed resistance to multiple antibiotics, and the susceptibility pattern of the isolates to different antibiotics was tested and interpreted using the disk diffusion method (Kirby-Bauer) described by the Clinical and Laboratory Standards Institute. It was observed that the organisms were susceptible to Imipenem, Nitrofurantoin, Ciprofloxacin, Azithromycin and Augumentin. Moreso, of the identified Vibrio species large percentage showed resistance to Tetracycline and Ampicillin. This study clearly indicated and concluded that the meat and the effluents discharged from these abattoirs to the environment are reservoir for Vibrio species as well as antibiotics resistance genes. The high distribution of resistant vibrios in these abattoirs shows potential public health hazards. Therefore, there is an urgent need to establish a sanitation and hygienic intervention majorly focused on limiting the rate of contamination of the slaughtered animal in causing human illness when in contact with, and also to adopt a strategies that are effective in eliminating antibiotics resistant Vibrios species.

 

Keywords: Abattoir; Vibrio Species; Antibiotics Resistance; Effluent; Meat

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References

  1. Mehdi R., et al. “Occurrence of Vibrio in lobster and crab from the Persian Gulf”. Journal of Food Safety 32 (2012): 198-203.
  2. Maiti B., et al. “Evaluation Of Rapd-Pcr And Protein Profile Analysis To Differentiate Vibrio Harveyi Strains Prevalent Along The Southwest Coast Of India”. Journal of Genetics 88 (2009): 273-279.
  3. Passalacqua P L., et al. “Occurrence of Vibrio parahaemolyticus, Vibrio cholerae and Vibrio vulnificus in the Clam Ruditapes Philippinarum (Adams & Reeve, 1850) from Emilia Romagna and Sardinia, Italy”. Italian Journal of Food Safety 5 (2016): 5709.
  4. Iroha IR., et al. “Bacterial contamination of raw meat sold in Abakaliki, Ebonyi State Nigeria”. Journal of Public Health and Epidemiology 3 (2011): 49-53.
  5. Odjadjare EE., et al. “Multidrug-resistant Vibrio species isolated from abattoir effluents in Nigeria”. Journal of Infection in Developing Countries 5 (2017): 373-378.
  6. Olaoye OA and Onilude AA. “Investigation on the potential application of biological agents in the extension of shelf life of fresh beef in Nigeria”. World Journal of Microbiology and Biotechnology 26 (2010): 1445-1454.
  7. Koutsoumanis KP and Sofos JN. “Comparative acid stress response of Listeria monocytogenes, Escherichia coli O157: H7 and Salmonella Typhimurium after habituation at different pH conditions”. Letters in Applied Microbiology4 (2004): 321-326.
  8. Hassan AN., et al. “Microbial contamination of raw meat and its environment in retail shops in Karachi, Pakistan”. Journal of Infection in Developing Countries 4 (2010): 382-388.
  9. Fratamico PM., et al. “Foodborne pathogens in microbiology and molecular biology”. Caister Academic Press, Wymondham, Norfolk, UK (2015): 273.
  10. Farzana K., et al. “Prevalence and antibiotic resistance of bacteria in two ethnic milk based products”. Pakistan Journal of Botany 41 (2009): 935-943.
  11. Atieno NR., et al. “Isolation of high antibiotic resistant faecal bacteria indicators, Salmonella and Vibrio species from raw abattoirs sewage in peri-urban locations of Nairobi, Kenya, Greener”. Journal of Biological Sciences5 (2013): 172-178.
  12. Ogbonna DN. “Distribution of microorganisms in water, soils and sediment from abattoir wastes in southern Nigeria”. International Journal of Current Microbiology and Applied Sciences 9 (2014): 1183-1200.
  13. Cheesbrough M. Microbiological test. In District Laboratory Practice in Tropical Countries; Part II. New York, NY, USA: Cambridge University Press (2000).
  14. Ottaviani D., et al. “A biochemical protocol for the isolation and identification of current species of Vibrio in seafood”. Journal of Applied Microbiology6 (2003): 1277-1284.
  15. Daniels NA and Shafaie A. “A review of pathogenic Vibrio infections for clinicians”. Infections in Medicine10 (2000): 665-685.
  16. Osunla CA and Okoh AI. “Vibrio pathogens: A public health concern in rural water resources in sub-Saharan Africa”. International Journal of Environmental Research and Public Health10 (2017): 1188.
  17. Chowdhury G., et al. “Vibrio fluvialis in patients with diarrhea, Kolkata, India”. Emerging Infectious Diseases11 (2012): 1868.
  18. Kraemer SA., et al. “Antibiotic pollution in the environment: from microbial ecology to public policy”. Microorganisms6 (2019): 180.
  19. Neela FA., et al. “The diversity of multi-drug resistance profiles in tetracycline-resistant Vibrio species isolated from coastal sediments and seawater”. Journal of Microbiology-Seoul1 (2007): 64.
  20. Bhattacharya SK. “An evaluation of current cholera treatment”. Expert Opinion on Pharmacotherapy2 (2003): 141-146.
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Citation

Citation: Osunla Charles Ayodeji. “Distribution and Antibiotic Susceptibility Profiles of Vibrio Species Isolated from Selected Abattoirs in Akoko South-West, Ondo State, Nigeria". Acta Scientific Microbiology 4.11 (2021): 25-29.




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