Acta Scientific Microbiology (ISSN: 2581-3226)

Research Article Volume 4 Issue 10

Internal Quality Control of Neisseria Meningitidis Carriage in Kaya and Boussouma Region of Center-North Burkina Faso from 2016 to 2017

Sampo E5*, Sanou M4, Sanon B2, Somlare H1, Ouattara K1, Kienou M1, Sawadogo M1, Ouedraogo A1, Kabre A2, Bagaya S2, Traore B2, Ake F3, Tarbangdo F3, Nagalo BM7, Kristiansen PA6, Barro N5, Ouedraogo R4 and Sangare L1

1Yalgado OUEDRAOGO University Hospital Center, Ouagadougou, Burkina Faso
2KAYA Regional Hospital Center, Center-Nord, Burkina Faso
3DAVYCAS International , Ouagadougou, Burkina Faso
4Charles DE GAULLE University Hospital Center, Ouagadougou, Burkina Faso
5Joseph KI-ZERBO University, (LaBESTA), Ouagadougou, Burkina Faso
6Coalition for Epidemic Preparedness Innovations (CEPI), Oslo, Norway
7Department of Hematology/Oncologie, Mayo Clinic Scottsdale, AZ, USA

*Corresponding Author: Sampo E, Joseph KI-ZERBO University, (LaBESTA), Ouagadougou, Burkina Faso.

Received: August 04, 2021 ; Published: September 23, 2021


Background: Internal quality control is essential for preventing inaccuracy of disease causing microbial diagnostic, poor reagent quality and equipment dysfunction in a meningeal bacteria carriage study. This study aimed to assess the quality of meningococcal carriage study results obtained from Boussouma and Kaya health districts in the Center-North of Burkina Faso from 2016 to 2017.

Methodology: During the N. meningitidis carriage study, internal quality control was performed on culture media, field working conditions, reagents, laboratory equipment and antibiotics discs.

Result: During the carriage study, an evaluation of culture media was made. Thus, no defective or contaminated modified Thayer-Martin (TMM) were not recorded. On the other hand, we noted 86 defective fresh sheep blood agar (GSF) media and 201 other contaminated. Evaluation of sample collection conditions showed that the field ambient temperature was between + 22-+ 37°C. The duration of field sampling varied respectively from 77-173 minutes, 74-232 minutes, 74-195 minutes and 75-210 minutes at first, second, third and fourth campaign. The transport duration between sampling sites and the laboratory varied between 26-118 minutes, 21-187 minutes, 27-121 minutes and 25-137minutes at first, second, third and fourth campaigns. Thus, the delay of samples transmission from the first sample collection until all TMM media laboratory incubation varied respectively 145-264 minutes, 120-264 minutes, 126-288 minutes and 120-301 minutes at first, second, third and fourth campaign. TMM and GSF sterility control did not show any contamination. We did not observe any colony on TMM media with Proteus mirabilis (NC 04175) and Staphylococcus aureus (ATCC 25923) sowed for selectivity control. On the other hand, growth colonies were observed on TMM and GSF media with N. meningitidis A (ATCC 13077) and N. lactamica (ATCC 23970) reference strains. Reagents quality control showed that N. meningitidis A is Gamma-glutamyl-beta-naphthylamide (GGT) and oxidase positive and O-Nitrophenyl β-D-galactopyranoside (ONPG) negative. On the other hand, N. lactamica hydrolyzes ONPG, possesses cytochrome oxidase and is devoid of GGT. Incubator temperature varied between + 36.3- +36.7°C. Bacteria identification control showed good growth and suitable antisera agglutination with N. meningitidis A (ATCC 13077) reference strain. The strain storage temperature in the freezer ranged from -78--66°C. The refrigerator temperature used for reagents and media storage varied between + 2- +8°C. Finally, antibiotic discs tested gave good sensitivity with N. meningitidis A reference strain.

Conclusion: internal quality control system improved Neisseria meningitidis diagnosis in meningococcal A carriage study. It allowed assessing bacteriological diagnosis to obtain quality and reliable results in meningococcal A carriage study at CHR of Kaya laboratory in Burkina Faso from 2016 to 2017.

Keywords: Quality Control; Meningococcal A Carriage; Burkina Faso


  1. Lapeysonie L. “La méningite cérébrospinale en Afrique”. Bulletin of the World Health Organization 28 (1963): 3-114.
  2. Rouphael NG and Stephens DS. “Neisseria meningitidis: Biology, Microbiology, and Epidemiology”. Methods in Molecular Biology 799 (2012): 1-20.
  3. Koumare B., et al. “The first large epidemic of meningococcal disease caused by serogroup W135. Burkina Faso in 2002”. Vaccine 25 (2007): A 37 -A41.
  4. Funk A., et al. “Sequential outbreaks due to a new strain of Neisseria meningitidis serogroup C in northern Nigeria, 2013-14”. PLoS Currents Outbreaks 6 (2014): 1-7.
  5. Djobo S., et al. “Outbreaks of serogroups X meningococcal meningitis in Niger 1995-2000”. Tropical Medicine and International Health12 (2003): 1118-1123.
  6. Nicolas P., et al. “Molecular Epidemiology of Neisseria meningitidis Isolated in the African Meningitis Belt between 1988 and 2003 Shows Dominance of Sequence Type 5 (ST-5) and ST-11 Complexes”. Journal of Clinical Microbiology 43 (10): 5129-5135.
  7. Yazdankhah SP., et al. “Neisseria meningitidis: an overview of the carriage state”. Journal of Medical Microbiology 53 (2004): 821-832.
  8. Maclennan J., et al. “Social behavior and meningococcal carriage in british teenagers”. Emerging Infectious Diseases 6 (2006): 950-957.
  9. Kritiansen PA., et al. “Baseline meningococcal carriage in Burkina Faso before the introduction of a meningococcal serogroup A conjugate Vaccine”. Clinical Vaccine Immunology3 (2011): 435-443.
  10. Sow SO., et al. “Immunogenicity and safety of a meningococcal a conjugate vaccine in Africans”. The New England Journal of Medicine 364 (2011): 2293-2304.
  11. LaForce FM., et al. “Eliminating epidemic group, A meningococcal meningitis in Africa through a new vaccine”. Health Affair (Millwood) 30 (2011): 1049-1057.
  12. “Réseau de la méningite épidémique”. Département des Maladies transmissibles: surveillance et action (consulté le 30/04/2019) (2005).
  13. Sidikou F., et al. “Emergence of epidemic Neisseria meningitidis serogroup C in Niger, 2015: an analysis of national surveillance data”. Lancet Infectious Diseases 11 (2016): 1288-1294.
  14. Diallo AO., et al. “Bacterial meningitis epidemiology and return of Neisseria serogroup A cases in Burkina Faso in the five years following MenAfriVac mass vaccination campaign”. PLoS One11 (2017): e0187466.
  15. Kristiansen PA., et al. “Laboratory quality control in a multicentre meningococcal carriage study in Burkina Faso”. Transactions of the Royal Society of Tropical Medicine and Hygiene 106 (2012): 189-297.
  16. Bauer AW., et al. “Antibiotic susceptibility testing by a standardized single disk method”. American Journal of Clinical Pathology4 (1966): 493-496.
  17. Thayer JD., et al. “Thayer-Martin milieu sélectif pour la culture de Neisseria meningitidis à partir du nasopharynx”. American Journal of Public Health 6 (1965): 923-927.
  18. “Meningitis control in countries of the African meningitis belt”. 16 (2015): 209-216.
  19. Roberts J., et al. “Sampling methods to detect carriage of Neisseria meningitidis”. Journal of Infection 58 (2009): 103-107.
  20. Massicotte L. “Laboratoire de santé publique du Québec-INSPQ. Les milieux de culture, ces négligés”. AMMIQale1 (2003): 25-26.
  21. Martin JE., et al. “New system for cultivation of Neisseria gonorrhea”. Applied and Environmental Microbiology 27 (1974): P.802-805.
  22. Takahashi H and Watanabe H. “Post-translational processing of Neisseria meningitides gamma-glutamyl aminopeptidase and its association with inner membrane facing to the cytoplasmic space”. FEMS Microbiology Letter 234 (2004): 27-35.
  23. Kristiansen PA., et al. “Persistent low carriage of serogroup A Neisseria meningitidis two years after mass vaccination with the meningococcal conjugate vaccine, MenAfriVac”. BMC Infectious Diseases 14 (2014): 663.
  24. Ryan KJ., et al. “Neisseria. An Introduction to Infectious Diseases”. United States. Sherris Medical Microbiology (2004): 327-341.
  25. Desautels J., et al. Guide de microbiologie. Ordre professionnel des technologistes médicaux du Québe (2017): P20-21.
  26. Isenberg HD. “Clinical Microbiology Procedures Handbook”. Washington DC. ASM Press 1 2e éd, (2004): 2298.
  27. Performance Standards for Antimicrobial Disk Susceptibility Tests, Approved Standard, Pennsylvania. Clinical and laboratory standards institute. Ninth Edition (2006): M2-A9.
  28. Craven DE., et al. “Serogroup identification of Neisseria meningitidis: comparison of an antiserum agar method with bacterial slide agglutination”. Journal of Clinical Microbiology 5 (1978): 410-414.


Citation: Sampo E., et al. “Characterization and Growth Evaluation of Marine Chlorella sp. for Biomass Production”. Acta Scientific Microbiology 4.10 (2021): 80-91.


Copyright: © 2021 Sampo E., et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.


Acceptance rate33%
Acceptance to publication20-30 days

Indexed In

News and Events

Contact US