Isolation of Protease Producing Strains and Evaluation of Protease Assay from Fecal Water and Curd Samples
Assistant Professor, Department of Chemical Sciences, Sree Vidyanikethan Degree College, AP, India
*Corresponding Author: Eswari Beeram, Assistant Professor, Department of Chemical Sciences, Sree Vidyanikethan Degree College, AP, India.
Received: July 31, 2021 ; Published: August 12, 2021
E. coli and yeast strain S. cerevisiae are the well known microbiota in the microbial world and can be easily isolated from the contaminated water bodies. So, we have chosen these two organisms because of their abundant availability, ease of plating and culturing on media. Proteases are most important in detergent industry and it is easy to isolate protease in large quantities from E. coli and Yeast strain S. cerevisiae and can recover the protease with low cost downstream processing methods. This article mainly focuses on the preliminary results about the secretion of protease in to the medium by fungi S. cerevisiae without requirement for autolysis and found to be cost effective. The protease can be recovered easily by simple pelleting of cells at 4000rpm for 15min.
Keywords: Gram Staining; S. cerevisiae; Methylene blue; Autolysis; Protease; Autolysis; Ammonium Hydroxide; Toulene
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