Umeaku Chinyelu Nkiru¹*, Chimezie Christian Chukwudi¹, Ozoh Chinwendu Njideka², Nwajiobi Favour Okwukweka², Abana Chioma Chineze² and Umeaku-Uzor Ugonwa Chiagoziem³

¹Department of Microbiology, Chukwuemeka Odumegwu Ojukwu University, Uli Campus, Nigeria
²Department of Applied Microbiology and Brewing, Nnamdi Azikiwe University, Nigeria
³Department of Mass Communications, Chukwuemeka Odumegwu Ojukwu University, Igbariam Campus, Nigeria

*Corresponding Author: Umeaku Chinyelu Nkiru, Department of Microbiology, Chukwuemeka Odumegwu Ojukwu University, Uli Campus, Nigeria.

Received: July 03, 2021 ; Published: August 05, 2021

Abstract

The isolation and genetic analysis of multi-resistant bacteria of patients suffering from urinary tract infections in Owerri metropolis of Imo State, Nigeria was evaluated. A total of 50 urine samples were collected from Federal Medical Centre, Owerri. Streak plate method of cultivation of microorganisms was adopted in the different selective media used; MacConkey, Salmonella shigella, mannitol salt and eosin methylene blue agars. Nutrient agar was used for the sensitivity. Out of the 50 samples, 9 were male and 41 were females. The patients aged between 20 and 40 years of age. Results revealed the following organisms; Escherichia coli, Staphylococcus aureus, Salmonella spp., Klebsiella spp., and Pseudomonas aeruginosa. E. coli had the highest occurrence of 56%, followed by S. aureus with 26%, Salmonella with 8%, while Klebsiella and Pseudomonas aeruginosa had 6% and 4% respectively. The isolates were resistant to most of the antibioitics used. Sp (Sparfloxacin) showed the highest zone of inhibition with a diameter of 23mm, and the lowest is 1.0 mm (CN:Gentamycin). E. coli was mostly resistant to SXT (Septrin), CH (Chloramphenical), CN (Gentamycin), S (Streptomycin), and GPX (Tarivid). Klebsiella was resistant to SXT (Septrin), CH (Chloramphenical), CN (Gentamycin), S (Streptomycin), and GPX (Tarivid). Pseudomonas was resistant to SXT (Septrin), CH (Chloramphenicol) and S. aureus was only resistant to SXT (Septrin), AM (Amoxacillin), and R (Rocephine). The organisms show high resistance to SXT (Septrin), CH (Chloramphenical), CN (Gentamycin), GPX (Tarivid), and S (Streptomycin). Plasmid extraction and Agarose gel electrophoresis was done to determine the presence of 16SrRNA gene, CTX-M gene, BlaTEM gene, SHV gene and OX gene on Escherichia coli and Salmonella spp and the result shows 1500bp of 16s gene at 100bp molecular ladder for 16SrRNA gene, 550bp gene bands of CTX-M at 100bp molecular ladder of 1500bp, 401bp of Bla TEM gene bands at 100bp molecular ladder of 1500bp and 293bp of SHV gene bands at 100pb molecular ladder of 1500bp, with OX gene showing no band. The bacteria studied contain antibiotic resistant genes. Therefore, indiscriminate use of antibiotics should be avoided.

Keywords: Escherichia coli; Salmonella; Isolation; Owerri Metropolis

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Citation

Citation: Umeaku Chinyelu Nkiru and Ezechinwoye Chiemelie Lauretta. “Isolation of Multi-Resistant Bacteria of Patients Suffering from Urinary Tract Infections in Owerri Metropolis, Imo State, Nigeria ”. Acta Scientific Microbiology 4.9(2021): 03-16.

Copyright

Copyright: © 2021 Umeaku Chinyelu Nkiru and Ezechinwoye Chiemelie Lauretta. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.