Umeaku Chinyelu Nkiru* and Ezechinwoye Chiemelie Lauretta
Department of Microbiology, Chukwuemeka Odumegwu Ojukwu University, Uli, Anambra, Nigeria
*Corresponding Author: Umeaku Chinyelu Nkiru, Department of Microbiology, Chukwuemeka Odumegwu Ojukwu University, Uli, Anambra, Nigeria.
Received: June 11, 2021 ; Published:
Chromolaena odorata and honey were used against Staphylococcus aureus and Escherichia coli obtained from wounds to determine the antibacterial effects of both agents against the test isolates. The study was done in the Project Laboratory of Chukwuemeka Odumegwu Ojukwu University, Uli. The isolates were obtained from wound of the pupils of Community Primary School, Umuoma, Uli and characterized using Microbiological and biochemical tests. The leaves of C. odorata were aseptically air dried, ground and prepared in aqueous and ethanoic solutions. Crude extract was used. Fresh honey obtained from Ogidi market, Idemili North were prepared in different concentrations. Agar well diffusion and Minimum inhibitory concentration (MIC) was used to determine the therapeutic activity. The result revealed that honey exhibited antibacterial activities against the test organisms and zones of inhibition were obtained showing high antibacterial activity with significant increase in the concentration of the honey. Different solvent such as ethanol and cold water were used for the extraction of C. odorata leaves. Crude undiluted extract were also used against the test organisms. Ethanolic extract showed to be more effective than crude and cold water extracts. The MIC of ethanolic extract against S. aureus and E. coli were 62.5 mg/ml and 125 mg/ml respectively. The MIC of the C. odorata ethanoic extract and zone of inhibition obtained from different concentrations of honey and different extracts of C. odorata compares favorably with the commercially available Augmentin which justifies the therapeutic use of these agents in the medical field for treatment of ulcers, bed sores and other infections resulting from burns and wounds.
Keywords: Chromolaena odorata; Staphylococcus aureus; Escherichia coli; Honey; Ethanol and Cold Water
Citation: Umeaku Chinyelu Nkiru and Ezechinwoye Chiemelie Lauretta. “Antibacterial Effect of Honey and Chromolaena odorata on Escherichia coli and Staphylococcus aureus Isolated from Wound Infection ”. Acta Scientific Microbiology 4.8 (2021): 158-164.
Copyright: © 2021 Umeaku Chinyelu Nkiru and Ezechinwoye Chiemelie Lauretta. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.