Acta Scientific Microbiology (ISSN: 2581-3226)

Research Article Volume 4 Issue 8

Antibacterial Effect of Honey and Chromolaena odorata on Escherichia coli and Staphylococcus aureus Isolated from Wound Infection

Umeaku Chinyelu Nkiru* and Ezechinwoye Chiemelie Lauretta

Department of Microbiology, Chukwuemeka Odumegwu Ojukwu University, Uli, Anambra, Nigeria

*Corresponding Author: Umeaku Chinyelu Nkiru, Department of Microbiology, Chukwuemeka Odumegwu Ojukwu University, Uli, Anambra, Nigeria.

Received: June 11, 2021 ; Published:

Abstract

Chromolaena odorata and honey were used against Staphylococcus aureus and Escherichia coli obtained from wounds to determine the antibacterial effects of both agents against the test isolates. The study was done in the Project Laboratory of Chukwuemeka Odumegwu Ojukwu University, Uli. The isolates were obtained from wound of the pupils of Community Primary School, Umuoma, Uli and characterized using Microbiological and biochemical tests. The leaves of C. odorata were aseptically air dried, ground and prepared in aqueous and ethanoic solutions. Crude extract was used. Fresh honey obtained from Ogidi market, Idemili North were prepared in different concentrations. Agar well diffusion and Minimum inhibitory concentration (MIC) was used to determine the therapeutic activity. The result revealed that honey exhibited antibacterial activities against the test organisms and zones of inhibition were obtained showing high antibacterial activity with significant increase in the concentration of the honey. Different solvent such as ethanol and cold water were used for the extraction of C. odorata leaves. Crude undiluted extract were also used against the test organisms. Ethanolic extract showed to be more effective than crude and cold water extracts. The MIC of ethanolic extract against S. aureus and E. coli were 62.5 mg/ml and 125 mg/ml respectively. The MIC of the C. odorata ethanoic extract and zone of inhibition obtained from different concentrations of honey and different extracts of C. odorata compares favorably with the commercially available Augmentin which justifies the therapeutic use of these agents in the medical field for treatment of ulcers, bed sores and other infections resulting from burns and wounds.

Keywords: Chromolaena odorata; Staphylococcus aureus; Escherichia coli; Honey; Ethanol and Cold Water

References

  1. Bowler HD. “Signs of clinical infection by stable polymicrobial communities”. Clinical Wound Management 3 (2001): 179.
  2. Jonathan H M., et al. “Microbial safety assessment of honey bees”. Book Microbiology (2007): 146.
  3. Thomas S and McCubbin P. “An in-vitro analysis of the antimicrobial properties of 10 silver containing dressing”. Wound Care 12 (2003): 305-308.
  4. Benkeblia N. “Phenolic compounds of Chromolaena odorata protect cultured skin cells from oxidative damage. Implication for Cutaneous Wound Healing”. Biological and Pharmaceutical Bulletin 12 (2001): 1373-1379.
  5. Arora DR and Arora BB. “Enterobactericeae: Escherichia, Klebsiella, and other genera”. Textbook of Microbiology, Fourth Edition, CBS Publishers and Distributors Pvt Limited, New Delhi, India (2012): 345-351.
  6. Zhenxiang LU., et al. “Isolation, identification and charaterisation of novel Bacillus subtilis”. Journal of Veterinary Medical Science3 (2018): 427-433.
  7. Snelling SL., et al. “Rapid colorimetric methods to detect indole”. Journal of Food Science4 (2006): 1548-1553.
  8. Niemi RM., et al. “Confirmation of Esherichia Coli and its distinction from Klebsiella species by gas and indole formation at 44 and 44.50C”. Journal of Applied Microbiology6 (2003): 1242-1249
  9. Kanika S. “Mannual of Microbiology: t Tools and techniques” (2007).
  10. Wiliex DJ., et al. “A comparison of the sensitivity of the wound – infecting species of bacteria to the antibacterial activity of manuka honey and other honey”. Journals of Applied Bacteriology3 (2009): 88-98.
  11. Blair B. “Inhibitory effect of honey bee collected pollen on most cell degranulation Chemical Composition in-vitro”. Journal of Medicinal Food1 (2004): 14-20.
  12. Naibe NS., et al. “Assessment of techniques of antimicrobial properties of natural compounds of plant orign”. African Journal of Biotechnology12 (2008): 1797-1896.
  13. Nostro A., et al. “Extraction methods, and bio autography for evaluation of medicinal plant antimicrobial activity”. Literature of Applied Microbiology 30 (2000): 379-384.
  14. Willey JM., et al. “Isolation of pure cultures”. Prescott, Harley and Klein’s Microbiology, Seventh Edition, McGraw Hill Companies, New York (2008): 113-117.

Citation

Citation: Umeaku Chinyelu Nkiru and Ezechinwoye Chiemelie Lauretta. “Antibacterial Effect of Honey and Chromolaena odorata on Escherichia coli and Staphylococcus aureus Isolated from Wound Infection ”. Acta Scientific Microbiology 4.8 (2021): 158-164.

Copyright

Copyright: © 2021 Umeaku Chinyelu Nkiru and Ezechinwoye Chiemelie Lauretta. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.




Metrics

Acceptance rate33%
Acceptance to publication20-30 days
Impact Factor0.810

Indexed In



News and Events


  • Certification for Review
    Acta Scientific certifies the Editors/reviewers for their review done towards the assigned articles of the respective journals.
  • Submission Timeline for Upcoming Issue
    The last date for submission of articles for regular Issues is October 20, 2021.
  • Publication Certificate
    Authors will be issued a "Publication Certificate" as a mark of appreciation for publishing their work.
  • Best Article of the Issue
    The Editors will elect one Best Article after each issue release. The authors of this article will be provided with a certificate of “Best Article of the Issue”.
  • Welcoming Article Submission
    Acta Scientific delightfully welcomes active researchers for submission of articles towards the upcoming issue of respective journals.
  • Contact US