Effects of Copper Sulfate and Zinc Sulfate on Cell Adhesion of Staphylococcus aureus and Aeromonas hydrophila Stemming from Different Cell Growth Phases in Aquatic Microcosm
Romeo Tagne Fodouop1, Brice Hermann Fokouong Tcholong1,2, Antoine Tamsa Arfao1*, Ahmadou Fadimatou3, Joseph Patrick Atangana Kouna1, Guy Epole Etame1, Chrétien Lontsi Djimeli1, Paul Alain Nana4, Geraud Canis Tasse Taboue2 Télesphore Sime-Ngando5 and Nola Moise1
1University, Country1Hydrobiology and Environment Laboratory, University of Yaounde 1, Faculty of Sciences, Cameroon
2Bangangte Multipurpose Station, Scientific Coordination Forest, Soil and Environment, Institute of Agricultural Research for Development Cameroun, Yaoundé, Cameroun, Central Africa and Department of Aquatic Ecosystems Management, Institute of Fisheries and Aquatic Sciences, University of Douala, Douala, Cameroon
3Laboratory S3MN2E, Faculty of Sciences, Mohammed-V University Rabat, Morocco
4Department of Oceanography and Limnology, Institute of Fisheries and Aquatic Sciences, University of Douala, Cameroon
5Laboratoire "Microorganismes: Génome et Environnement (LMGE)", Université Clermont Auvergne, Aubière Cedex, France
*Corresponding Author: Antoine Tamsa Arfao, University, Country1Hydrobiology and Environment Laboratory, University of Yaounde 1, Faculty of Sciences, Cameroon.
Received: June 11, 2021 ; Published: July 23, 2021
This study aimed at evaluating the effect of different concentrations of zinc sulfate and copper sulfate on bacterial adhesion A. hydrophila and S. aureus polyethylene at different stages of growth, different exposure times and monitor the pH and conductivity during the adhesion test. The analyzes showed that the maximum abundance of adhered cells were obtained in the lag growth phase and exponential growth phase. A negative and significant difference was observed between the abundance of adherent cells and concentrations of heavy metal salts. Indeed, in every condition of experience, it was noted that increasing the incubation period lead to a significant increase in abundance of bacterial cells adhered to polyethylene, so the increase in the concentrations of heavy metal salts significantly decreases the abundance of adherent cells. These results suggest that the incubation period, growth phase and the concentrations of heavy metal salts have an influence on the adhesion of S. aureus and A. hydrophila in polyethylene. The use of heavy metals with small concentration can be an additional process, in reduction of planktonic cells using adhesion process.
Keywords: Cell Growth Phases; Bacteria Adhesion; Polyethylene; Heavy Metals
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