Acta Scientific Microbiology (ISSN: 2581-3226)

Research Article Volume 4 Issue 7

Detection of Human Cytomegalovirus in Tissue Samples of Colorectal Cancer in Brazzaville Hospital University Center, Congo

Dorine Florence Luthera Ngombe Mouabata1, Anicet Luc Magloire Boumba1,2, Gervillien Arnold Malonga1*, Dimitry Moudiongui Mboungou Malanda1,4, Patrina Joseph Iloukou Mayakia1, Donatien Moukassa1,3 and Jean Felix Peko1,4

1Department of Virology and Molecular Viral Oncology, Faculty of Health Sciences, Marien Ngouabi University, Brazzaville, Republic of Congo
2Medical Analysis Laboratory, General Hospital of LOANDJILI, Pointe- Noire, Republic of Congo
3Department of Anatomy and cytopathology, General Hospital Edith Lucie Bongo Ondimba, Oyo, Republic of Congo
4Department of Anatomy and Cytopathology, University Hospital Center, Brazzaville, Republic of Congo

*Corresponding Author: Gervillien Arnold Malonga, Department of Virology and Molecular Viral Oncology, Faculty of Health Sciences, Marien Ngouabi University, Brazzaville, Republic of Congo.

Received: May 06, 2021 ; Published: June 07, 2021


Background: Colorectal cancer (CRC) is the most common gastrointestinal cancer and the third leading cause of cancer death and morbidity worldwide. Human cytomegalovirus (HCMV) has been implicated as a potential etiopathogenetic agent. He is considered as an oncomodulator virus because of its effects on cell-cycle progression, mutagenesis, angiogenesis, and immune evasion. We conducted this study with the objective of detecting human cytomegalovirus by Nested Polymerase Chain Reaction.

Methods: In this study, 41 colorectal cance formalin-fixed paraffin-embedded (FFPE) tissue samples diagnosed on histological basis were included. DNA was extracted from these tissues for detection of HCVM DNA virus using Nested Polymerase Chain Reaction. Statistical analysis was performed with Chi-square test.

Results: In 6 cases of colorectal cancer tissue samples (6/41, 14.6%), HCMV DNA was detected. The mean age was 53 years. The histological type of the patients was associated with the positivity of human cytomegalovirus with a statistically significant difference (p = 0.041).

Conclusion: The HCMV virus could play a role in creating malignancy and the progression of cancer through the process of oncomodulation. He has a contributing role in colorectal cancer; although more study is required to clearly define its involvement in this type of cancer.

Keywords: Human Cytomegalovirus; Colorectal Cancer; Nested-PCR; Brazzaville; Congo


  1. Bray Freddie., et al. “Global Cancer Statistics 2018: GLOBOCAN Estimates of Incidence and Mortality Worldwide for 36 Cancers in 185 Countries”. CA: A Cancer Journal for Clinicians6 (2018): 394‑424.
  2. Belhamidi Mohamed Said., et al. “Profil épidémiologique et anatomopathologique du cancer colorectal: à propos de 36 cas”. The Pan African Medical Journal159 (2018): 159.
  3. Hunt R H., et al. “The Stomach in Health and Disease”. Gut 10 (2015): 1650‑1668.
  4. Fearon Eric R and Bert Vogelstein. “A Genetic Model for Colorectal Tumorigenesis”. Cell5 (1990): 759‑767.
  5. Enam Sahnila., et al. “Association of Human Polyomavirus JCV with Colon Cancer: Evidence for Interaction of Viral T-Antigen and Beta-Catenin”. Cancer Research23 (2002): 7093‑7101.
  6. Damin D C., et al. “Evidence for an Association of Human Papillomavirus Infection and Colorectal Cancer”. European Journal of Surgical Oncology: The Journal of the European Society of Surgical Oncology and the British Association of Surgical Oncology5 (2007): 569‑574.
  7. Mesri Enrique A., et al. “Human viral oncogenesis: a cancer hallmarks analysis”. Cell Host and Microbe3 (2014): 266‑282.
  8. Chen Hsin-Pai., et al. “Genetic Polymorphisms of the Human Cytomegalovirus UL144 Gene in Colorectal Cancer and Its Association with Clinical Outcome”. Journal of General Virology12 (2015): 3613‑3623.
  9. Mazeron M C., et al. “Infections à cytomégalovirus”. EMC - Maladies infectieuses2 (2009): 1‑19.
  10. Dowd Jennifer Beam and Allison Aiello. “Socioeconomic Differentials in Immune Response in the U.S”. Epidemiology (Cambridge, Mass.) 20.6 (2009): 902‑908.
  11. Cannon Michael J., et al. “Review of Cytomegalovirus Seroprevalence and Demographic Characteristics Associated with Infection”. Reviews in Medical Virology4 (2010): 202‑213.
  12. Michaelis Martin., et al. “The Story of Human Cytomegalovirus and Cancer: Increasing Evidence and Open Questions”. Neoplasia (New York, N.Y.) 11.1 (2009): 1‑9.
  13. Castillo Jonathan P and Timothy F Kowalik. “Human Cytomegalovirus Immediate Early Proteins and Cell Growth Control”. Gene1‑2 (2002): 19‑34.
  14. Cinatl J., et al. “Modulatory Effects of Human Cytomegalovirus Infection on Malignant Properties of Cancer Cells”. Intervirology 4 (1996): 259‑269.
  15. Powers C., et al. “Cytomegalovirus Immune Evasion”. Current Topics in Microbiology and Immunology 325 (2008): 333‑359.
  16. Harkins Lualhati E., et al. “Detection of Human Cytomegalovirus in Normal and Neoplastic Breast Epithelium”. Herpesviridae 1 (2010): 8.
  17. Chen HP., et al. “Tumoral Presence of Human Cytomegalovirus Is Associated with Shorter Disease-Free Survival in Elderly Patients with Colorectal Cancer and Higher Levels of Intratumoral Interleukin-17”. Clinical Microbiology and Infection7 (2014): 664‑671.
  18. Huang E S and J K Roche. “Cytomegalovirus D.N.A. and Adenocarcinoma of the Colon: Evidence for Latent Viral Infection”. Lancet (London, England)8071 (1978): 957‑960.
  19. Akintola-Ogunremi Olaronke., et al. “Is Cytomegalovirus Associated With Human Colorectal Tumorigenesis?” American Journal of Clinical Pathology2 (2005): 244‑249.
  20. Boguszaková L., et al. “Absence of Cytomegalovirus, Epstein-Barr Virus, and Papillomavirus DNA from Adenoma and Adenocarcinoma of the Colon”. Acta Virologica4 (1988): 303‑308.
  21. Cai Zhen-Zhai., et al. “Human Cytomegalovirus-Encoded US28 May Act as a Tumor Promoter in Colorectal Cancer”. World Journal of Gastroenterology9 (2016): 2789.
  22. Dimberg Jan., et al. “Detection of Cytomegalovirus DNA in Colorectal Tissue from Swedish and Vietnamese Patients with Colorectal Cancer”. Anticancer Research11 (2013): 4947‑4950.
  23. Li Xutong., et al. “Upregulation of Toll‑like receptor 2 expression in colorectal cancer infected by human cytomegalovirus”. Oncology Letters1 (2015): 365‑370.
  24. Salyakina Daria and Nicholas F Tsinoremas. “Viral Expression Associated with Gastrointestinal Adenocarcinomas in TCGA High-Throughput Sequencing Data”. Human Genomics 7 (2013): 23.
  25. Sole Claudio V., et al. “Human Cytomegalovirus and Epstein-Barr Virus Infection Impact on 18F-FDG PET/CT SUVmax, CT Volumetric and KRAS-Based Parameters of Patients with Locally Advanced Rectal Cancer Treated with Neoadjuvant Therapy”. European Journal of Nuclear Medicine and Molecular Imaging2 (2015): 186‑196.
  26. Tafvizi Farzaneh and Zahra Tahmasebi Fard. “Detection of Human Cytomegalovirus in Patients with Colorectal Cancer by Nested-PCR”. Asian Pacific Journal of Cancer Prevention3 (2014): 1453‑1457.
  27. Karimi Maryam., et al. “Relative Frequency of Cytomegalovirus (CMV) in Tissue Samples of Women with Breast Cancer in Sanandaj, Iran”. International Journal of Bioassays03 (2016): 4907.
  28. Kühn J E., et al. “Quantitation of Human Cytomegalovirus Genomes in the Brain of AIDS Patients”. Journal of Medical Virology1 (1995): 70‑82.
  29. Mehrabani-Khasraghi Sahar., et al. “Analysis of Colorectal Cancer and Polyp for Presence Herpes Simplex Virus and Cytomegalovirus DNA Sequences by Polymerase Chain Reaction”. Journal of Analytical Research in Clinical Medicine2 (2016): 82‑89.
  30. Kalejta Robert F and Thomas Shenk. “Proteasome-dependent, ubiquitin-independent degradation of the Rb family of tumor suppressors by the human cytomegalovirus pp71 protein”. Proceedings of the National Academy of Sciences 6 (2003): 3263‑3268.
  31. Al‐Sohaily Sam., et al. “Molecular Pathways in Colorectal Cancer”. Journal of Gastroenterology and Hepatology9 (2012): 1423‑1431.
  32. Shen Zhiyuan. “Genomic instability and cancer: an introduction”. Journal of Molecular Cell Biology1 (2011): 1‑3.
  33. Bender Cecilia., et al. “Analysis of colorectal cancers for human cytomegalovirus presence”. Infectious Agents and Cancer1 (2009): 1‑6.


Citation: Gervillien Arnold Malonga., et al. “Detection of Human Cytomegalovirus in Tissue Samples of Colorectal Cancer in Brazzaville Hospital University Center, Congo ”. Acta Scientific Microbiology 4.7 (2021): 32-37.


Copyright: © 2021 Gervillien Arnold Malonga., et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.


Acceptance rate33%
Acceptance to publication20-30 days

Indexed In

News and Events

  • Certification for Review
    Acta Scientific certifies the Editors/reviewers for their review done towards the assigned articles of the respective journals.
  • Submission Timeline for Upcoming Issue
    The last date for submission of articles for regular Issues is July 30, 2024.
  • Publication Certificate
    Authors will be issued a "Publication Certificate" as a mark of appreciation for publishing their work.
  • Best Article of the Issue
    The Editors will elect one Best Article after each issue release. The authors of this article will be provided with a certificate of "Best Article of the Issue"
  • Welcoming Article Submission
    Acta Scientific delightfully welcomes active researchers for submission of articles towards the upcoming issue of respective journals.

Contact US