Detection of Human Cytomegalovirus in Tissue Samples of Colorectal Cancer in Brazzaville Hospital University Center, Congo
Dorine Florence Luthera Ngombe Mouabata1, Anicet Luc Magloire Boumba1,2, Gervillien Arnold Malonga1*, Dimitry Moudiongui Mboungou Malanda1,4, Patrina Joseph Iloukou Mayakia1, Donatien Moukassa1,3 and Jean Felix Peko1,4
1Department of Virology and Molecular Viral Oncology, Faculty of Health Sciences, Marien Ngouabi University, Brazzaville, Republic of Congo
2Medical Analysis Laboratory, General Hospital of LOANDJILI, Pointe- Noire, Republic of Congo
3Department of Anatomy and cytopathology, General Hospital Edith Lucie Bongo Ondimba, Oyo, Republic of Congo
4Department of Anatomy and Cytopathology, University Hospital Center, Brazzaville, Republic of Congo
*Corresponding Author: Gervillien Arnold Malonga, Department of Virology and Molecular Viral Oncology, Faculty of Health Sciences, Marien Ngouabi University, Brazzaville, Republic of Congo.
Received: May 06, 2021 ; Published: June 07, 2021
Background: Colorectal cancer (CRC) is the most common gastrointestinal cancer and the third leading cause of cancer death and morbidity worldwide. Human cytomegalovirus (HCMV) has been implicated as a potential etiopathogenetic agent. He is considered as an oncomodulator virus because of its effects on cell-cycle progression, mutagenesis, angiogenesis, and immune evasion. We conducted this study with the objective of detecting human cytomegalovirus by Nested Polymerase Chain Reaction.
Methods: In this study, 41 colorectal cance formalin-fixed paraffin-embedded (FFPE) tissue samples diagnosed on histological basis were included. DNA was extracted from these tissues for detection of HCVM DNA virus using Nested Polymerase Chain Reaction. Statistical analysis was performed with Chi-square test.
Results: In 6 cases of colorectal cancer tissue samples (6/41, 14.6%), HCMV DNA was detected. The mean age was 53 years. The histological type of the patients was associated with the positivity of human cytomegalovirus with a statistically significant difference (p = 0.041).
Conclusion: The HCMV virus could play a role in creating malignancy and the progression of cancer through the process of oncomodulation. He has a contributing role in colorectal cancer; although more study is required to clearly define its involvement in this type of cancer.
Keywords: Human Cytomegalovirus; Colorectal Cancer; Nested-PCR; Brazzaville; Congo
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