Microbiological Investigation of Water Samples from Intensive Care Unit (ICU) of Tertiary Care Hospitals of Dhaka, Bangladesh
Shah Murshid UJ Jaman Arowan, Abdihakim Ismail Ali and Saurab Kishore Munshi*
Department of Microbiology, Stamford University Bangladesh, Dhaka, Bangladesh
*Corresponding Author: Saurab Kishore Munshi, Department of Microbiology, Stamford University Bangladesh, Dhaka, Bangladesh.
Received: May 23, 2021; Published: June 07, 2021
Water could be a crucial transmitter of nosocomial infections in patients who are admitted to hospitals’ Intensive Care Unit (ICU). The present study was undertaken to evaluate the microbiological quality of different water samples from ICU of tertiary care hospitals for measuring their potential of causing nosocomial infections. In this regard, drinking and tap water samples were collected from the ICU of three tertiary care hospitals located in Dhaka, Bangladesh. Total viable bacteria were present in an average of 3 log10 cfu/ml in all the samples. Among the specific bacterial isolates, Pseudomonas spp. and Bacillus spp. were predominant and encountered in all samples. E. coli contamination was observed in almost all water samples collected of the three ICUs. Staphylococcus spp. and Klebsiella spp. were recovered in water samples from ICU-1 and ICU-2. Fecal coliforms and Vibrio spp. were alarmingly found in the tap water samples of ICU-1. A few of the isolates from the water samples of ICUs were able to form biofilm on the Congo red media that accelerate the risk. Moreover, the bacterial isolates were found to be multidrug resistant. Higher resistance was found against Cefixime, Amoxicillin, Nalidixic Acid, Cephradine and Piperacillin. Overall, The findings of the present study reveal that the tested ICU water samples could be a potential source of nosocomial infections by the drug-resistant bacteria.
Keywords: Intensive Care Unit; Nosocomial Infection; Drinking Water; Water Quality; Bacterial Pathogens; Drug Resistance
- Dasgupta S., et al. “Nosocomial infections in the intensive care unit: Incidence, risk factors, outcome and associated pathogens in a public tertiary teaching hospital of Eastern India”. Indian Journal of Critical Care Medicine1 (2015): 14-20.
- Hopman J., et al. “Reduced rate of intensive care unit acquired gram-negative bacilli after removal of sinks and introduction of ‘water-free’ patient care”. Antimicrobial Resistance and Infection Control 6 (2017): 59.
- Maltezou HC., et al. “Infection control practices in facilities for highly infectious diseases across Europe”. Journal of Hospital Infection3 (2012):184-191.
- Dancer SJ. “Controlling hospital-acquired infection: focus on the role of the environment and new technologies for decontamination”. Clinical Microbiology Reviews4 (2014): 665-690.
- Gonsu KH., et al. “Bacteriological assessment of the hospital environment in two referral hospitals in Yaoundé-Cameroon”. The Pan African Medical Journal 20 (2015): 224.
- Anaissie EJ., et al. “The hospital water supply as a source of nosocomial infections”. Archives of Internal Medicine13 (2002):1483-1492.
- Mara D., et al. “Sanitation and health”. PLoS Medicine11 (2010): e1000363.
- Kirankumar Y., et al. “Bacteriological analysis of water samples from different points in a tertiary care hospital”. Journal of Clinical and Diagnostic Research19 (2019): DC04-DC09.
- Ashbolt NJ. “Microbial contamination of drinking water and disease outcomes in developing regions”. Toxicology 198 (2004): 229-238.
- Zamxaka M., et al. “Microbiological and physico-chemical assessment of the quality of domestic water sources in selected rural communities of the Eastern Cape Province, South Africa”. Water SA 30 (2004): 333-340.
- Trautmann M., et al. “Tap water colonization with Pseudomonas aeruginosain a surgical intensive care unit (ICU) and relation to Pseudomonas infections of ICU patients”. Infection Control and Hospital Epidemiology 22 (2001): 2249-2252.
- Perkins KM., et al. “Investigation of healthcare infection risks from water-related organisms: Summary of CDC consultations, 2014—2017”. Infection Control and Hospital Epidemiology6 (2019): 621-626.
- Mansour O., et al. “Analyze study of water in emergency departments at general hospitals of the Syrian coast”. Research Journal of Pharmacy and Technology 1 (2017): 1-6.
- Nahar A., et al. “Isolation of Acinetobacter species and their antibiotic resistance pattern in an intensive care unit (ICU) of a tertiary care hospital in Dhaka, Bangladesh”. Bangladesh Journal of Medical Microbiology1 (2012): 3-6.
- Islam T., et al. “Bacterial propagation in municipal water and deep tube-well water in kashipur locality of Narayanganj City, Bangladesh”. Journal of Water and Environment Technology5 (2020): 327-337.
- Acharjee M., et al. “Bacterial proliferation in municipal water supplied in Mirpur locality of Dhaka city, Bangladesh”. Clean − Soil Air Water4 (2014): 434-441.
- Munshi SK., et al. “Detection of virulence potential of diarrheagenic Escherichia coli isolated from surface water rivers surrounding Dhaka city”. Journal of Bangladesh Academy of Sciences1 (2012): 109-121.
- Ahmed T., et al. “Microbiological study of drinking water: qualitative and quantitative approach”. Asian Journal of Microbiology, Biotechnology and Environmental Sciences4 (2013): 23-30.
- Hossaini F., et al. “Antimicrobial traits of different medicinal plants locally available in Bangladesh”. Biomedical and Biotechnology Research Journal1 (2021): 1-6.
- Hossaini F., et al. “Antimicrobial effects of different extracts of medicinally used green leafy vegetables collected from local market of Dhaka”. Food Research3 (2020): 860-865.
- Akter M., et al. “Microbiological quality assessment of ready-to-eat fried chicken and chicken soup samples sold in Dhaka Metropolis, Bangladesh”. Sumerianz Journal of Biotechnology7 (2019): 48-54.
- Akter A., et al. “Occurrence of drug resistant bacteria in household waste samples”. Stamford Journal of Microbiology1 (2019): 15-18.
- Munshi SK., et al. “Influence of multi-species biofilm formed in vitro from different environmental samples on the drug-resistance traits of resident bacteria”. Bangladesh Journal of Microbiology2 (2018): 108-114.
- Kaiser TDL., et al. “Modification of the Congo red agar method to detect biofilm production by Staphylococcus epidermidis”. Diagnostic Microbiology and Infectious Disease 3 (2013): 235-239.
- Das NC., et al. “Influence of tannery wastes and the surrounding environment of tannery industries on microbial growth and proliferation”. Stamford Journal of Microbiology1 (2017): 14-18.
- Leitner E., et al. “Contaminated handwashing sinks as the source of a clonal outbreak of KPC-2-producing Klebsiella oxytoca on a hematology ward”. Antimicrobial Agents and Chemotherapy 59.1 (2015): 714-716.
- Guyot A., et al. “Outbreak of Stenotrophomonas maltophilia on an intensive care unit”. Journal of Hospital Infection4 (2013): 303-307.
- Grundmann H., et al. “Pseudomonas aeruginosain a neonatal intensive care unit: reservoirs and ecology of the nosocomial pathogen”. Journal of Infectious Disease4 (1993): 943-947.
- Barrell R., et al. “Microbiological standards for water and their relationship to health risk”. Communicable Disease and Public Health 3 (2000): 8-13.
- Eckmanns T., et al. “An outbreak of hospital-acquired Pseudomonas aeruginosa infection caused by contaminated bottled water in intensive care units”. Clinical Microbiology and Infection 14 (2008): 454-458.
- Decker BK and Palmore TN. “Hospital water and opportunities for infection prevention”. Current Infectious Disease Report10 (2014): 432.
- Ng C., et al. “Characterization of Metagenomes in urban aquatic compartments reveals high prevalence of clinically relevant antibiotic resistance genes in wastewaters”. Frontiers in Microbiology 8 (2017): 2200.
- Yao Y., et al. “Insights into a novel blaKPC-2-encoding IncP-6 plasmid reveal Carbapenem-resistance circulation in several Enterobacteriaceae species from wastewater and a hospital source in Spain”. Frontiers in Microbiology 8 (2017):1143.
- Vergara-Lopez S., et al. “Wastewater drainage system as an occult reservoir in a protracted clonal outbreak due to metallo-beta-lactamase-producing Klebsiella oxytoca”. Clinical Microbiology and Infection11 (2013): E490-498.