Acta Scientific Microbiology (ISSN: 2581-3226)

Research Article Volume 4 Issue 6

Validation of Optimized Method for Quantitative Estimation of Extracellular Ethanol from Micro-algae

Nishant Saxena1, Preeti Singh1, Debanjan Sanyal1* and Santanu Dasgupta2

1Research and Development, Reliance Industries Ltd, Jamnagar, India
2Research and Development, Reliance Corporate Park, Ghansoli, Navi-Mumbai, India

*Corresponding Author: Debanjan Sanyal, Research and Development, Reliance Industries Ltd, Jamnagar, India.

Received: April 01, 2021 ; Published: May 17, 2021

Abstract

Identification and quantification of ethanol in biological samples such as algae, is of paramount importance for research and quality laboratories. An accurate quantification with minimum standard deviation is still a challenging task due to high interference of salts, ions, metals and biological contaminations. A rapid method for the determination of ethanol content in algae samples was validated for seven parameters using gas chromatography. The study was conducted here, to confirm it’s specificity, linearity, accuracy, precision, robustness, sensitivity, and suitability for ethanol estimation in micro-algae samples. During specificity analysis, retention time of ethanol were found 3.27 minutes for standard and 3.25 minutes for sample, which were statistically similar. The linearity of this method for ethanol estimation was found till 5000 ppm for both standard and sample and R2 were 1 and 0.9953 respectively. In the recovery study, the 100% ethanol were recovered from standard and sample with a standard deviation of less than 2%. Repeatability study demonstrated that, the method was found more precise and observed < 1% standard deviation for peak retention time and peak area, similarly < 2% standard deviation was observed among the 10 replicates of standard. In the ruggedness analysis, significant differences were not observed on ethanol content by different analysts on different days of analysis. In the robustness analysis the variation of flow rate, oven temperature and column did not affect the final results of ethanol content significantly. It was found from sensitivity analysis that, this analytical method can detect > 1.36 ppm and can accurately quantify > 4.13 ppm ethanol of algae sample. Validated data indicated that, GC-FID-HSS method is more specific, linear, accurate, repeatable, rugged, robust and sensitive for ethanol estimation. Hence this method can be used effectively to estimate ethanol content in algae samples without any interference from ions, salts, metals and biological contamination.

Keywords: Accuracy; Algae Sample; DB Wax; Ethanol Content; GC-FID-HS

References

  1. Brennan L and Owende P. “Biofuels from micro-algae—a review of technologies for production, processing, and extractions of biofuels and co-products”. Renewable and Sustainable Energy Reviews 14 (2010): 557-577.
  2. Nigam PS and Singh A. “Production of liquid biofuels from renewable resources”. Progress in Energy and Combustion Science 37 (2011): 52-68.
  3. Mata TM., et al. “Microalgae for biodiesel production and other applications: a review”. Renewable and Sustainable Energy Reviews 14 (2010): 217-232.
  4. Demirbas A. “Comparison of transesterification methods for production of biodiesel from vegetable oils and fats”. Energy Conversion and Management 49 (2008): 125-130.
  5. Song M., et al. “Marine brown algae: a conundrum answer for sustainable biofuels production”. Renewable and Sustainable Energy Reviews 50 (2015): 782-792.
  6. Kecebas A and Alkan MA. “Educational and consciousness-raising movements for renewable energy in Turkey”. Energy Education Science and Technology 1 (2009): 157-170.
  7. Fulton LT., et al. “Biofuels for transport: An international perspective”. Paris: International Energy Agency (IEA) (2004).
  8. Hill J., et al. “Environmental, economic, and energetic costs and benefits of biodiesel and ethanol biofuels”. Proceedings of the National Academy of Sciences of the United States of America 103 (2006): 11206-11210.
  9. Goldemberg J and Guardabassi P. “The potential for first generation ethanol production from sugarcane”. Biofuels Bioproducts and Biorefining 4 (2010): 17-24.
  10. Cuellar-Bermudez SP., et al. “Photosynthetic bioenergy utilizing CO2: an approach on flue gases utilization for third generation biofuels”. Journal of Cleaner Production 98 (2015): 53-65.
  11. Burton T., et al. “A review of the potential of marine algae as a source of biofuel in Ireland”. Dublin: Sustainable Energy Ireland- SEI (2009).
  12. Yen HW and Brune DE. “Anaerobic co-digestion of algal sludge and waste paper to produce methane”. Bioresource Technology 98 (2007): 130-134.
  13. Laurens LM., et al. “Algal biomass constituent analysis: method uncertainties and investigation of the underlying measuring chemistries”. Analytical Chemistry4 (2012): 1879-188.
  14. Vergara-Fernandez A., et al. “Evaluation of marine algae as a source of biogas in a two-stage anaerobic reactor system”. Biomass and Bioenergy 32 (2008): 338-344.
  15. Nakas JPS., et al. “System development for linked-fermentation production of solvents from algal biomass”. Applied and Environmental Microbiology 46 (1983): 1017-1023.
  16. Cheng-Wu Z., et al. “An industrial-size flat plate glass reactor for mass production of Nannochloropsis sp. (Eustigmatophyceae)”. Aquaculture 195 (2001): 35-49.
  17. Besada V., et al. “Heavy metals in edible seaweeds commercialised for human consumption”. Journal of Marine Systems 75 (2009): 305-313.
  18. Spolaore P., et al. “Commercial applications of microalgae”. Journal of Bioscience and Bioengineering 101 (2006): 87-96.
  19. Demirbas MF. “Biofuels from algae for sustainable development”. Applied Energy10 (2011): 3473-3480.
  20. John RP., et al. “Micro and microalgal biomass: a renewable source for bioethanol”. Bioresource Technology 102 (2011): 186-193.
  21. Donald TM. “Ethanol fuel use in Brazil”. 16th International Symposium on Alcohol Fuels (2006).
  22. Tiscione NB., et al. “Ethanol Analysis by Headspace Gas Chromatography with Simultaneous Flame-Ionization and Mass Spectrometry Detection”. Journal of Analytical Toxicology 35 (2011): 501-511.
  23. ICH Guideline. “Q2B, Validation of analytical procedures: methodology”. in: Proceedings of the International Conference on Harmonization (1996).
  24. Helena P., et al. “GC Determination of Acetone, Acetaldehyde, Ethanol, and Methanol in Biological Matrices and Cell Culture”. Journal of Chromatographic Science4 (2009): 272-278.
  25. Wang ML., et al. “A Rapid Method for Determination of Ethanol in Alcoholic Beverages Using Capillary Gas Chromatography”. Journal of Food and Drug Analysis2 (2003): 133-140.
  26. Argasiska JG., et al. “Development and validation of gc-fid method for The determination of ethanol residue in marjoram ointment”. Acta Poloniae Pharmaceutican Drug Research6 (2009): 611-615.
  27. Batista LR and Antoniosi Filho NR. “Ethanol Content Determination in Medicine Syrups Using Headspace and Multidimensional Heart-Cut Gas Chromatography Coupled to Mass Spectrometry”. Journal of the Brazilian Chemical Society 2 (2020): 394-401.

Citation

Citation: Debanjan Sanyal., et al. “Validation of Optimized Method for Quantitative Estimation of Extracellular Ethanol from Micro-algae ”. Acta Scientific Microbiology 4.6 (2021): 28-37.

Copyright

Copyright: © 2021 Debanjan Sanyal., et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.




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