Aoi Koga, Nao Yamauchi, Mayu Imamura, Mina Urata, Tomomi Kurayama, Ranko Iwai, Shuhei Hayashi, Shinjiro Yamamoto and Hitoshi Miyasaka*
Department of Applied Life Science, Sojo University, Nishiku, Japan
*Corresponding Author: Hitoshi Miyasaka, Department of Applied Life Science, Sojo University, Nishiku, Japan.
Received: December 30, 2020 ; Published: January 28, 2021
The marine non-sulfur purple photosynthetic bacterium Rhodovulum sulfidophilum has a wide application potential in the fields of aquaculture, renewable energy production, environmental protection, and biomaterial production. To detect, identify and classify various R. sulfidophilum strains, we designed a PCR primer set targeting pufC gene encoding one of the photosystem proteins. Nucleotide sequence alignment of the pufC genes from five R. sulfidophilum strains revealed that the 3’ region of this gene is rich in nucleotide substitutions (approximately 10 substitutions/100 bp), making it suitable for the identification and classification of various R. sulfidophilum strains. We designed a primer set that amplified 0.7 kb of the 3’ region of pufC gene. For the validation of this primer set, we used fish fecal DNA as the PCR templates, and successfully identified and classified several R. sulfidophilum strains.
Keywords: Photosynthetic Bacteria; Rhodovulum sulfidophilum; PCR; Fish Fecal DNA
Citation: Hitoshi Miyasaka., et al. “pufC Gene Targeted PCR Primers for Identification and Classification of Marine Photosynthetic Bacterium Rhodovulum sulfidophilum”. Acta Scientific Microbiology 4.2 (2021): 69-75.
Copyright: © 2021 Hitoshi Miyasaka., et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.