Acta Scientific Microbiology (ISSN: 2581-3226)

Research Article Volume 4 Issue 2

pufC Gene Targeted PCR Primers for Identification and Classification of Marine Photosynthetic Bacterium Rhodovulum sulfidophilum

Aoi Koga, Nao Yamauchi, Mayu Imamura, Mina Urata, Tomomi Kurayama, Ranko Iwai, Shuhei Hayashi, Shinjiro Yamamoto and Hitoshi Miyasaka*

Department of Applied Life Science, Sojo University, Nishiku, Japan

*Corresponding Author: Hitoshi Miyasaka, Department of Applied Life Science, Sojo University, Nishiku, Japan.

Received: December 30, 2020 ; Published: January 28, 2021

×

Abstract

The marine non-sulfur purple photosynthetic bacterium Rhodovulum sulfidophilum has a wide application potential in the fields of aquaculture, renewable energy production, environmental protection, and biomaterial production. To detect, identify and classify various R. sulfidophilum strains, we designed a PCR primer set targeting pufC gene encoding one of the photosystem proteins. Nucleotide sequence alignment of the pufC genes from five R. sulfidophilum strains revealed that the 3’ region of this gene is rich in nucleotide substitutions (approximately 10 substitutions/100 bp), making it suitable for the identification and classification of various R. sulfidophilum strains. We designed a primer set that amplified 0.7 kb of the 3’ region of pufC gene. For the validation of this primer set, we used fish fecal DNA as the PCR templates, and successfully identified and classified several R. sulfidophilum strains.

Keywords: Photosynthetic Bacteria; Rhodovulum sulfidophilum; PCR; Fish Fecal DNA

×

References

  1. Loo PL., et al. “Rhodovulum sulfidophilum, A Phototrophic Bacterium, Grown in Palm Oil Mill Effluent Improves the Larval Survival of Marble Goby Oxyeleotris Marmorata (Bleeker)”. Aquaculture Research3 (2013): 495-507.
  2. Chang BV., et al. “Investigation of a Farm-Scale Multitrophic Recirculating Aquaculture System with the Addition of Rhodovulum sulfidophilum for Milkfish (Chanos chanos) Coastal Aquaculture”. Sustainability (Switzerland)7 (2019).
  3. Mukkata K., et al. “Diversity of Purple Nonsulfur Bacteria in Shrimp Ponds with Varying Mercury Levels”. Saudi Journal of Biological Sciences4 (2016): 478-487.
  4. Azad SA., et al. “Rhodovulum sulfidophilum in the Treatment and Utilization of Sardine Processing Wastewater”. Letters in Applied Microbiology1 (2004): 13-18.
  5. Maeda I., et al. “Maximization of Hydrogen Production Ability in High-Density Suspension of Rhodovulum sulfidophilum Cells Using Intracellular Poly(3-Hydroxybutyrate) as Sole Substrate”. Biotechnology and Bioengineering4 (2003): 474-481.
  6. Cai J and Guangce W. “Hydrogen Production by a Marine Photosynthetic Bacterium, Rhodovulum sulfidophilum P5, Isolated from a Shrimp Pond”. International Journal of Hydrogen Energy20 (2012): 15070-15080.
  7. Foong CP., et al. “Optimal Iron Concentrations for Growth-Associated Polyhydroxyalkanoate Biosynthesis in the Marine Photosynthetic Purple Bacterium Rhodovulum sulfidophilum under Photoheterotrophic Condition”. PLoS ONE4 (2019): 1-12.
  8. Kikuchi Y and Umekage S. “Extracellular Nucleic Acids of the Marine Bacterium Rhodovulum sulfidophilum and Recombinant RNA Production Technology Using Bacteria”. FEMS Microbiology Letters3 (2018).
  9. Foong CP., et al. “A Marine Photosynthetic Microbial Cell Factory as a Platform for Spider Silk Production”. Communications Biology1 (2020).
  10. Fujimoto H., et al. “Whole-Cell Arsenite Biosensor Using Photosynthetic Bacterium Rhodovulum Sulfidophilum”. Applied Microbiology and Biotechnology2 (2006): 332-338.
  11. Yamauchi N., et al. “Isolation of A Marine Purple Non-Sulfur Photosynthetic Bacterium With A High Ability of Glycerol Assimilation”. International Journal of Plant, Animal and Environmental Sciences4 (2019): 214-221.
  12. Dereeper A., et al. “Phylogeny.Fr: Robust Phylogenetic Analysis for the Non-Specialist”. Nucleic Acids Research 36 (2008).
  13. Masuda S., et al. “A New Cytochrome Subunit Bound to the Photosynthetic Reaction Center in the Purple Bacterium, Rhodovulum sulfidophilum”. Journal of Biological Chemistry16 (1999): 10795-10801.
  14. Tank M., et al. “Phylogenetic Relationship of Phototrophic Purple Sulfur Bacteria According to PufL and PufM Genes”. International Microbiology3 (2009): 175-185.
  15. Yutin N., et al. “Novel Primers Reveal Wider Diversity among Marine Aerobic Anoxygenic Phototrophs”. Applied and Environmental Microbiology12(2005): 8958-8962.
  16. Baldo L., et al. “Phylogeography and Ecological Niche Shape the Cichlid Fish Gut Microbiota in Central American and African Lakes”. Frontiers in Microbiology 10 (2019): 1-19.
  17. Ye Q., et al. “Effects of Dietary Gelsemium elegans Alkaloids on Intestinal Morphology, Antioxidant Status, Immune Responses and Microbiota of Megalobrama amblycephala”. Fish and Shellfish Immunology 94 (2019): 464-78.
  18. Huang Z., et al. “Microecological Koch’s Postulates Reveal That Intestinal Microbiota Dysbiosis Contributes to Shrimp White Feces Syndrome”. Microbiome1 (2020): 1-13.
  19. Yamazaki Y., et al. “Repeated Selective Enrichment Process of Sediment Microbiota Occurred in Sea Cucumber Guts”. Environmental Microbiology Reports6 (2019): 797-807.
  20. Yamazaki Y., et al. “Individual Apostichopus japonicus Fecal Microbiome Reveals a Link with Polyhydroxybutyrate Producers in Host Growth Gaps”. Scientific Reports 6 (2016): 1-10.
  21. Reddy CSK., et al. “Polyhydroxyalkanoates: An Overview”. Bioresource Technology2 (2003): 137-146.
  22. Schryver PD., et al. “Poly-β-Hydroxybutyrate (PHB) Increases Growth Performance and Intestinal Bacterial Range-Weighted Richness in Juvenile European Sea Bass, Dicentrarchus labrax”. Applied Microbiology and Biotechnology5 (2010): 1535-1541.
  23. Duan Y., et al. “Effect of Dietary Poly-β-Hydroxybutyrate (PHB) on Growth Performance, Intestinal Health Status and Body Composition of Pacific White Shrimp Litopenaeus vannamei (Boone, 1931)”. Fish and Shellfish Immunology 60 (2017): 520-528.
  24. Cristiane CP., et al. “Phylogenetically and Spatially Close Marine Sponges Harbour Divergent Bacterial Communities”. PLoS ONE12 (2012).
×

Citation

Citation: Hitoshi Miyasaka., et al. “pufC Gene Targeted PCR Primers for Identification and Classification of Marine Photosynthetic Bacterium Rhodovulum sulfidophilum”. Acta Scientific Microbiology 4.2 (2021): 69-75.




Metrics

Acceptance rate33%
Acceptance to publication20-30 days

Indexed In



News and Events


  • Certification for Review
    Acta Scientific certifies the Editors/reviewers for their review done towards the assigned articles of the respective journals.
  • Submission Timeline for Upcoming Issue
    The last date for submission of articles for regular Issues is December 25, 2024.
  • Publication Certificate
    Authors will be issued a "Publication Certificate" as a mark of appreciation for publishing their work.
  • Best Article of the Issue
    The Editors will elect one Best Article after each issue release. The authors of this article will be provided with a certificate of "Best Article of the Issue"

Contact US