Acta Scientific Microbiology (ISSN: 2581-3226)

Research Article Volume 4 Issue 2

Fabrication, Characterization, In Vitro Release, and Some Biological Activities of Eucalyptus Essential Oil Loaded Poly (Lactic Acid) Nanofibers

Hayfa Argui1,3*, Salih Can Suner2,5, Çağdaş Deniz Periz4, Seyhan Ulusoy4, Mossadok Ben-Attia3, Ayhan Oral2, Yalçın Coşkun6 , Ayhan Oral7 and Hechmi Said1

1Materials Chemistry Laboratory, Department of Chemistry, Faculty of Sciences of Bizerte, University of Carthage, Tunisia
2Department of Chemistry and Chemical Processing, Lapseki Vocational School, Canakkale Onsekiz Mart University, Turkey
3Biomonitoring of the Environment Laboratory (LR01/ES14), Faculty of Sciences of Bizerte, University of Carthage, Tunisia
4Department of Biology, Faculty of Science, Suleyman Demirel University, Turkey
5Canakkale Onsekiz Mart University Application in Science and Technology and Research Laboratory, Turkey
6Canakkale Onsekiz Mart University Department of Organic Farming, Lapseki Vocational College, Turkey
77Department of Chemistry, Faculty of Art and Science, Canakkale Onsekiz Mart University 17100 Çanakkale, Turkey.

*Corresponding Author: Hayfa Argui, Materials Chemistry Laboratory, Department of Chemistry, Faculty of Sciences of Bizerte, University of Carthage, Tunisia.

Received: December 29, 2020 ; Published: January 28, 2021

Reprints View PDF Related Articles

×

Abstract

The objective of this work was to evaluate antibacterial and antibiofilm activity of Eucalyptus camaldulensis EO-loaded PLA nanofibers. Different concentrations of E. camaldulensis EO (7.5, 10 and 15%) were incorporated into PLA nanofibers via an electrospinning technique. The nanofibers are characterized by Fourier transform infrared spectroscopy (FT-IR), scanning electron microscopy (SEM) and dynamic mechanical analysis (DMA). Antibacterial activity of essential oil-loaded PLA nanofibers against Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa and Enterococcus faecalis were investigated by disc diffusion method. The antibiofilm activity of essential oil-loaded PLA nanofibers was studied against Pseudomonas aeruginosa. The results obtained from the biofilm formation test revealed that PLA essential oil inhibited the Pseudomonas aeruginosa biofilm by 9.3%, 12.8% and 12.6%, respectively. Drug releasing behavior of essential oil-loaded PLA nanofibers was investigated by UV-Visible spectroscopy. According to releasing results, as the amounts of Eucalyptus camaldulensis essential oil in PLA nanofibers increased, the releasing decreased. The essential oil-loaded PLA nanofiber successfully delivered about 160 h.

Keywords: Eucalyptus camaldulensis Dehn; Electrospinning; Nanofibers; Drug Delivery; Antibacterial Activity; Antibiofilm Activity

×

References

  1. Greiner A and Wendorff JH. "Cover Picture: Electrospinning: A Fascinating Method for the Preparation of Ultrathin Fibers (Angew. Chem. Int. Ed. 30/2007)”. Angewandte Chemie International Edition 30 (2007): 5633-5633.
  2. Dzenis Y. "Spinning Continuous Fibers for Nanotechnology”. Science 5679 (2004): 1917-1919.
  3. Li D., et al. "Electrospinning: A Simple and Versatile Technique for Producing Ceramic Nanofibers and Nanotubes". Journal of the American Ceramic Society 6 (2006): 1861-1869.
  4. Frenot A and Chronakis IS. "Polymer Nanofibers Assembled by Electrospinning”. Current Opinion in Colloid nad Interface Science 1 (2003): 64-75.
  5. Ahn Y., et al. "Development of High Efficiency Nanofilters Made of Nanofibers”. Current Applied Physics 6 (2006): 1030-1035.
  6. Reyes-Jurado F., et al. "Essential Oils: Antimicrobial Activities, Extraction Methods, and Their Modeling”. Food Engineering Reviews 3 (2015): 275-297.
  7. López P., et al. "Development of Flexible Antimicrobial Films Using Essential Oils as Active Agents”. Journal of Agricultural and Food Chemistry 21 (2007): 8814-8824.
  8. Qin Y., et al. "Development of Active Packaging Film Made from Poly (Lactic Acid) Incorporated Essential Oil”. Progress in Organic Coatings 103 (2017): 76-82.
  9. Wang H., et al. "Diffusion and Antibacterial Properties of Nisin-Loaded Chitosan/Poly (L-Lactic Acid) Towards Development of Active Food Packaging Film”. Food and Bioprocess Technology 8 (2015): 1657-1667.
  10. Tsuji H. “Poly (Lactic Acid): Synthesis, Structures, Properties, Processing, and Applications”. Wiley (2011).
  11. Ghalem BR and Mohamed B. "Antibacterial Activity of Leaf Essential Oils of Eucalyptus Globulus and Eucalyptus Camaldulensis”. African Journal of Pharmacy and Pharmacology 10 (2008): 211-215.
  12. Khalaf ZZ and Zahra LA. "Evaluation of the Activity of Essential Oil and Hydrosol from Eucalyptus Camaldulensis against Some Bacterial Species”. Iraqi Journal of Science (2020): 1282-1288.
  13. Pharmacopée Européenne. Strasbourg Conseil De L’europe. Ed. edition, 4éme (2002).
  14. Im H., et al. "Combined Application of Bacterial Predation and Violacein to Kill Polymicrobial Pathogenic Communities”. Scientific Reports 1 (2017): 1-10.
  15. Angeles M., et al. "Emulsion Electrospinning: Composite Fibers from Drop Breakup During Electrospinning”. Polymers for Advanced Technologies 7 (2008): 728-33.
  16. Rieger KA and Schiffman JD. "Electrospinning an Essential Oil: Cinnamaldehyde Enhances the Antimicrobial Efficacy of Chitosan/Poly (Ethylene Oxide) Nanofibers”. Carbohydrate Polymers 113 (2014): 561-568.
  17. de Oliveira Mori CL., et al. "Electrospinning of Zein/Tannin Bio-Nanofibers”. Industrial Crops and Products 52 (2014): 298-304.
  18. Hosseini SF., et al. "Two-Step Method for Encapsulation of Oregano Essential Oil in Chitosan Nanoparticles: Preparation, Characterization and in Vitro Release Study”. Carbohydrate Polymers 1 (2013): 50-56.
  19. Hufford C., et al. "Two Antimicrobial Alkaloids from Heartwood of Liriodendron Tulipifera L”. Journal of Pharmaceutical Sciences 5 (1975): 789-792.
  20. Truchado P., et al. "Inhibition by Chestnut Honey of N-Acyl-L-Homoserine Lactones and Biofilm Formation in Erwinia Carotovora, Yersinia Enterocolitica, and Aeromonas Hydrophila”. Journal of Agricultural and Food Chemistry 23 (2009): 11186-11193.
  21. Dogan G., et al. "Chemical Composition and Biological Activities of Leaf and Fruit Essential Oils from Eucalyptus Camaldulensis”. Zeitschrift für Naturforschung C 11-12 (2017): 483-489.
  22. Sliti S., et al. "Leaf Essential Oils Chemical Composition, Antibacterial and Antioxidant Activities of Eucalyptus Camaldulensis and E. Rudis from Korbous (Tunisia)”. Journal of Materials and Environmental Science 3 (2015): 743-748.
  23. Mubarak EE., et al. "Essential Oil Compositions from Leaves of Eucalyptus Camaldulensis Dehn. And Callistemon Viminalis Originated from Malaysia”. IPCBEE 70 (2014): 137-141.
  24. Su Y-C., et al. "Antifungal Activities and Chemical Compositions of Essential Oils from Leaves of Four Eucalypts”. Taiwan Journal of Forest Science 1 (2006): 49-61.
  25. Cheng S-S., et al. "Chemical Compositions and Larvicidal Activities of Leaf Essential Oils from Two Eucalyptus Species”. Bioresource Technology 1 (2009): 452-456.
  26. Knezevic P., et al. "Antimicrobial Activity of Eucalyptus Camaldulensis Essential Oils and Their Interactions with Conventional Antimicrobial Agents against Multi-Drug Resistant Acinetobacter Baumannii”. Journal of Ethnopharmacology 178 (2016): 125-136.
  27. Ostad Asiaei E., et al. "Evaluation of Antimicrobial Activity of Eucalyptus Camaldulensis Essential Oil against the Growth of Drug-Resistant Bacteria”. Jundishapur Journal of Natural Pharmaceutical Products 4 (2018).
  28. Singh R and Thakur R. "Antibacterial Activity of Eucalyptus Camaldulensis against Bacteria Causing Food-Borne Illness”. The Indian Medical Gazette (2016): 195-99.
  29. Alghamdi AI and Ababutain IM. "Research Article Phytochemical Screening and Antibacterial Activity of Eucalyptus Camaldulensis’s Leaves and Bark Extracts” (2019).
  30. Traore N., et al. "Antimicrobial Activity of Essential Oils of Eucalyptus Camaldulensis from Mali”. Asian Journal of Plant Science and Research 4 (2014): 69-73.
  31. Javidi Z., et al. "Development of Flexible Bactericidal Films Based on Poly (Lactic Acid) and Essential Oil and Its Effectiveness to Reduce Microbial Growth of Refrigerated Rainbow Trout”. LWT-Food Science and Technology 72 (2016): 251-260.
  32. Pankongadisak P., et al. "The Use of Electrospun Curcumin-Loaded Poly (L-Lactic Acid) Fiber Mats as Wound Dressing Materials”. Journal of Drug Delivery Science and Technology 53 (2019): 101121.
  33. Kister G., et al. "Effects of Morphology, Conformation and Configuration on the Ir and Raman Spectra of Various Poly (Lactic Acid) S”. Polymer 2 (1998): 267-273.
  34. Inceboz T., et al. "In-Vivo and in-Vitro Tick Repellent Properties of Cotton Fabric”. Textile Research Journal 19 (2015): 2071-2082.
  35. Muller J., et al. "Poly (Lactic) Acid (Pla) and Starch Bilayer Films, Containing Cinnamaldehyde, Obtained by Compression Moulding”. European Polymer Journal 95 (2017): 56-70.
  36. Martino V., et al. "Thermal and Mechanical Characterization of Plasticized Poly (L-Lactide-Co-D, L-Lactide) Films for Food Packaging”. Journal of Thermal Analysis and Calorimetry 3 (2006): 707-712.
  37. Tawakkal IS., et al. "A Review of Poly (Lactic Acid)-Based Materials for Antimicrobial Packaging”. Journal of Food Science 8 (2014): R1477-1490.
  38. Mekonnen T., et al. "Progress in Bio-Based Plastics and Plasticizing Modifications”. Journal of Materials Chemistry A 43 (2013): 13379-98.
  39. Arrieta MP., et al. "Characterization of Pla-Limonene Blends for Food Packaging Applications”. Polymer Testing 4 (2013): 760-768.
  40. Zhang W., et al. "Electrospinning of Polylactic Acid Fibres Containing Tea Tree and Manuka Oil”. Reactive and Functional Polymers 117 (2017): 106-111.
  41. Nagarajan V., et al. "Perspective on Polylactic Acid (Pla) Based Sustainable Materials for Durable Applications: Focus on Toughness and Heat Resistance”. ACS Sustainable Chemistry and Engineering 6 (2016): 2899-2916.
  42. Vieira MGA., et al. "Natural-Based Plasticizers and Biopolymer Films: A Review”. European Polymer Journal 3 (2011): 254-263.
  43. Liu Y., et al. "Fabrication of Electrospun Polylactic Acid/Cinnamaldehyde/Β-Cyclodextrin Fibers as an Antimicrobial Wound Dressing”. Polymers 10 (2017): 464.
×

Citation

Citation: Hayfa Argui., et al. “Fabrication, Characterization, In Vitro Release, and Some Biological Activities of Eucalyptus Essential Oil Loaded Poly (Lactic Acid) Nanofibers”. Acta Scientific Microbiology 4.2 (2021): 54-63.




Metrics

Acceptance rate33%
Acceptance to publication20-30 days

Indexed In



News and Events


  • Certification for Review
    Acta Scientific certifies the Editors/reviewers for their review done towards the assigned articles of the respective journals.
  • Submission Timeline for Upcoming Issue
    The last date for submission of articles for regular Issues is December 25, 2024.
  • Publication Certificate
    Authors will be issued a "Publication Certificate" as a mark of appreciation for publishing their work.
  • Best Article of the Issue
    The Editors will elect one Best Article after each issue release. The authors of this article will be provided with a certificate of "Best Article of the Issue"

Contact US





Creative Commons License Open Access by Acta Scientific is licensed under a Creative Commons Attribution 4.0 International License
Based on a work at https://actascientific.com

ff

Acta-Publications

ff

© 2021 Acta Scientific, All rights reserved.

Creative Commons License Open Access by Acta Scientific is licensed under a Creative Commons Attribution 4.0 International License
Based on a work at https://actascientific.com

ff

Acta-Publications

ff

© 2021 Acta Scientific, All rights reserved.