Acta Scientific Microbiology (ASMI) (ISSN: 2581-3226)

Research Article Volume 3 Issue 12

Study of Herbal Antibiotics Derived from Turmeric and Ginger Applied on Enterobacteriaceae

Mehwish Saleem1*, Mashal Manzoor2, Sana Amjad2 and Aqsa Malik2

1PCSIR-Lab, Lahore, Pakistan
2Microbiology Lab Islamia College, Lahore, Pakistan

*Corresponding Author: Mehwish Saleem, PCSIR-Lab, Lahore, Pakistan.

Received: August 13, 2020; Published: November 11, 2020

×

Abstract

  Humans have been using natural products for medicinal use for ages. Natural products of therapeutic importance are compounds derived from plants, animals, or any microorganism. Ginger and Turmeric are also used as most commonly used condiments and natural drugs in vogue. These are traditional medicine, having some active ingredients used for the treatment of many diseases and killing of gram negative bacteria as well as gram positive i.e. E. coli, Klebsiella sp. Pseudomonas aeruginosa. Turmeric (Curcuma longa) and ginger (Zingiber officinale) has been used in cooking, and in herbal remedies. It’s possible mechanism of action was examined in terms of antioxidant availability during actual cooking conditions and in therapeutic applications using standardized extracts. The assays involve different levels of serial dilutions of ginger and turmeric and their activity on different stages of killing of bacteria. The aim of this study is to determine whether herbal antibiotics are efficient in their result of mortality in enterobacteriaceae. Aqueous, Methanolic and Ethanolic test extracts of Zingiber officinale and Curcuma longa were prepared. Microbiological tests were employed to determine the zone of inhibition. Standardization of isolates were obtained after incubating for 24 hours. Serial dilutions of Ginger and Turmeric were prepared to measure the OD value and growth of bacteria in the dilutions measured by spectrophotometer. The aqueous extracts of two major preparations of turmeric and ginger, corresponding to its use in cooking and medicine, shows significant antioxidant abilities. The studies reveal that the ability of ginger and turmeric having anti-inflammatory potential that shows susceptibility to gram negative bacteria in ethanol and methanol extract. The OD values of serial dilutions of Turmeric and Ginger determine the ratio of killing of bacteria in different concentrations. Thus it is concluded that Herbal antibiotics have a significant role in killing of bacteria. According to the research it is concluded that due to the excessive usage of medicines it is to be noted that the bacteria are shifting towards more resistancy. However usage of Herbal antibiotics can minimize this effect. Therefore scientists are shifting more towards Herbal medicines as compared to synthetic antibiotics.

Keywords: Turmeric; Ginger; Enterobacteriaceae

×

References

  1. Igbinosa OO., et al. “Antimicrobial activity and phytochemical screening of stem bark extracts from Jatropha curcas (Linn)”. African Journal of Pharmacy and Pharmacology2 (2009): 058-062.
  2. Vuorelaa P., et al. “Natural products in the process of finding new drug candidates”. Current Medicinal Chemistry11 (2004): 1375-1389.
  3. Adeshina GO., et al. “Antibacterial activity of fresh juices of Allium cepaand Zingiber officinale against multidrug resistant bacteria”. International Journal of Pharma and Bio Sciences 2 (2011): 289-295.
  4. Hamburger M and Hostettman K. “Bioactive an plants: The link between phytochemistry and medicine”. Phytochemistry 30 (1991): 3864-3874.
  5. Suffredini IB., et al. “Screening of antibacterial extracts from plants native to the Brazilian Amazon Rain Forest and Atlantic Forest”. Brazilian Journal of Medical and Biological Research 37 (2009): 379-384.
  6. Ríos JL and Recio MC. “Medicinal plants and antimicrobial activity”. Journal of Ethnopharmacology 100 (2005): 80-84.
  7. Amita S., et al. “Class1 integron and SXT Element in EI-Torstrains. Calcuta, India”. Emerging Infectious Diseases4 (2011): 500-507.
  8. Sebiomo A., et al. “Comparative studies of antibacterial effect of some antibiotics and ginger (Zingiber officinale) on two pathogenic bacteria”. Journal of Microbiology and Antimicrobial Agents 3 (2011): 18-22.
  9. Nirmala K., et al. “Dose-dependent effect in the inhibition of oxidative stress and anticlastogenic potential of ginger in STZ induced diabetic rats”. Food Chemistry 135 (2012): 2954-2959.
  10. McGee Harold. "A survey of tropical spices". McGee on Food and Cooking. Hodder and Stoughton (2004): 426.
  11. Park M., et al. “Antibacterial activity of [10]-gingerol and [12]-gingerol isolated from ginger rhizome against periodontal bacteria”. 22.11 (2008): 1446-1449.
  12. Singh P., et al. Mycobiology4 (2000): 185-189.
  13. Sofowora EA. “Medicinal Plants and Traditional Medicine in Africa”. John Wiley and Sons Ltd., Hoboken (1982): 64-79.
  14. Porkhel S., et al. “Comparison of antimicrobial activity of crude ethanolic extracts and essential oils of spices against five strains of diarrhea causing Escherichia coli”. International Journal of Pharmacy and Life Sciences 4 (2012): 1624-1627.
  15. Kunz and Brook. “Emerging resistant Gram-negative aerobic bacilli in hospital-acquired infections”. Chemotherapy6 (2010): 492-500.
  16. Aggarwal BB., et al. “Curcumin: an orally bioavailable blocker of TNF and other pro-inflam-matory biomarkers”. British Journal of Pharmacology8 (2013): 1672-1692.
  17. SINGH G., et al. “Chemistry, antioxidant and antimicrobial investigations on essential oil and oleoresins of Zingiber officinale”. In Food and Chemical Toxicology 46 (2008): 3295-3302.
  18. Michielin EM., et al. “Chemical composition and antibacterial activity of Cordia verbenacea extracts obtained by different methods”. Bioresource Tech 100 (2009): 6615-6623.
  19. RAHIMAN A., et al. “Antibacterial activity of natural spices on multiple drug resistant Eschericha coli isolated from drinking water, Bangladesh”. In Annals of Clinical Microbiology 10 (2011): 1-4.
  20. NANA SOMABAT S and LOHASUPTHAWEE P. “Antibacterial activity of crude ethanolic extracts and essential oils of spices against salmonellae and other enterobacteria”. In KMITL Science and Technology Journal 5 (2010): 527-538.
  21. IBRAHIM SA., et al. “Antimicrobial activity of Bifidobacterium Longum (CNCFB2259) as influenced by spices”. In Internet Journal of Food Safety 2 (2003): 6-8.
  22. Gull I., et al. “Inhibitory effect of Allium sativum and Zingiber officinale extracts on clinically important drug resistant pathogenic bacteria”. Annals of Clinical Microbiology and Antimicrobials 11 (2012): 8.
  23. , et al. “Extractive Value and Phytochemical Screening of Ginger (zingiber officinale) and Turmeric (curcuma longa) Using Different Solvents”. 8 (2018): 13-22.
  24. Kader G., et al. “Antimicrobial activities of the rhizome extract of Zingiber zerumbet Linn”. Asian Pacific Journal of Tropical Biomedicine 5 (2011): 409-412.
  25. Nweze E I., et al. “Antimicrobial activities of methanolic extracts of Trema guineensis (Schumm and Thorn) and Morinda lucida Benth used in Nigerian”. Journal of Biological Research and Biotechnology1 (2004): 39-46.
  26. O'Hara M., et al. “A review of 12 commonly used medicinal herbs”. Archives of Family Medicine6 (1998): 523-536.
  27. Singh R., et al. “Antibacterial activity of Curcuma longa rhizome extract on pathogenic bacteria research communications”. Current Science6 (2002): 738.
  28. Kim KJ., et al. “Antibacterial activity of Curcuma longa L. against methicillin-resistant Staphylococcus aureus”. Phytotherapy Research 19 (2005): 599-604.
  29. Negi PS., et al. “Antibacterial activity of turmeric oil: a by product from curcumin”. Journal of Agricultural and Food Chemistry 47 (1999): 4297-4300.
  30. Singh G., et al. “Chemistry, antioxidant and antimicrobial investigations on essential oil and oleoresins of Zingiber officinale”. Food and Chemical Toxicology 46 (2008): 3295-3302.
  31. Wang W., et al. “Simultaneous determination of 6-gingerol , 8-gingerol, 10-gingerol, 6-shaogol in rat plasma by liquid chromatography-mass spectrometry: Application to pharmacokinetics”. Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Science B 877 (2009): 671-679.
  32. Bajpai VK., et al. “Chemical composition, antibacterial and antioxidant activities of leaf essential oil and extracts of Metasequioa glyptostroboides Miki ex Hu”. Food Chemistry and Toxicology 47 (2009): 1876-1883.
  33. “Application to pharmacokinetics”. Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Science B 877: 671-679.
  34. Borges M Saavedra and M Simoes. “Insights on antimicrobial resistance, biofilms and the use of phytochemicals as new antimicrobial agents” (2015).
  35. Parveen Gul and Jehan Bakht. “Antimicrobial activity of turmeric extract and its potential use in food industry”. Journal of Food Science and Technology 4 (2015): 2272–2279.
×

Citation

Citation: Mehwish Saleem., et al. “Study of Herbal Antibiotics Derived from Turmeric and Ginger Applied on Enterobacteriaceae". Acta Scientific Microbiology 3.12 (2020): 07-15.




Metrics

Acceptance rate30%
Acceptance to publication20-30 days

Indexed In






News and Events


  • Certification for Review
    Acta Scientific certifies the Editors/reviewers for their review done towards the assigned articles of the respective journals.
  • Submission Timeline for Upcoming Issue
    The last date for submission of articles for regular Issues is December 25, 2024.
  • Publication Certificate
    Authors will be issued a "Publication Certificate" as a mark of appreciation for publishing their work.
  • Best Article of the Issue
    The Editors will elect one Best Article after each issue release. The authors of this article will be provided with a certificate of "Best Article of the Issue"

Contact US