Acta Scientific Gastrointestinal Disorders (ASGIS)(ISSN: 2582-1091)

Research Article Volume 5 Issue 6

Investigation of Optical Density of Venous Blood Plasma in Acute Intraabdominal Infection in the Experiment

Andrii I Shurma* and Fedir V Grynchuk

Department of Surgery № 1, Bukovynian State Medical University, Chernivtsi, Ukraine

*Corresponding Author: Andrii I Shurma, Department of Surgery № 1, Bukovynian State Medical University, Chernivtsi, Ukraine.

Received: April 13, 2022; Published: May 27, 2022

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Abstract

Background/Aims: Recognized laboratory criteria for acute intra-abdominal infection, in particular, general blood test, C-reactive protein, are nonspecific and insufficiently informative. We previously reported on the results of venous plasma optical density (ODVBP) measurements in emergency abdominal surgery. But the examined patients differed in the type of disease, sex, etc. Therefore, we conducted experimental studies to substantiate the possibility of using such a criterion.

Methods: 60 white nonlinear mature rats of both sexes, 180 to 200 g weight. To simulate acute intra-abdominal infection, intra-abdominal injection of 10 ml of 10% solution of autofaeces per 100 g of weight was performed. Blood from the jugular vein and pieces of peritoneum for histological examination was taken. The studies were performed before simulation, 6, 12, 24 and 48 h after simulation. ODVBP was examined on an Agilent Cary 100/300 Series UV-Vis spectrophotometer. Healthy animals were monitored for control.

Results: In healthy animals at a wavelength l = 310 nm there is a specific maximum ODVBP, reaching 0.22 units. After simulation of acute intra-abdominal infection and the occurrence of serous peritonitis, there was a statistically significant increase in ODVBP at this wavelength. After the onset of purulent peritonitis, ODVBP also increased statistically significantly. Subsequently, after the spread of inflammation to the deep tissues of the abdominal wall, ODVBP was at a high level and statistically significantly exceeded the control values.

Conclusions

  • In healthy white rats at a wavelength l = 310 nm, a maximum ODVBP was detected, reaching 0.22 units, which does not depend on sex differences.
  • After simulation of acute intra-abdominal infection and the development of serous inflammation of the peritoneum ODVBP this wavelength increased statistically significantly and reached 0.26 units.
  • After the development of purulent peritonitis, ODVBP again increased statistically significantly and reached 0.36 units, and then, after the spread of inflammation to the deep layers of the abdominal wall, the indicators were at a high level without significant differences.
  • The absolute parameters of the indicators differed from those determined in humans, but the ratios due to the presence of inflammatory changes in the abdominal cavity were preserved.

 

Keywords: Inflammation; Acute Intra-Abdominal Infection; Venous Blood Plasma; Optical Density of Venous Blood Plasma

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References

  • Di Saverio S., et al. “Diagnosis and treatment of acute appendicitis: 2020 update of the WSES Jerusalem guidelines”. World Journal of Emergency Surgery 27 (2020).
  • Rushing A., et al. “Management of acute appendicitis in adults: A practice management guideline from the Eastern Association for the Surgery of Trauma”. Journal of Trauma and Acute Care Surgery1 (2019): 214-224.
  • Leppäniemi A., et al. “2019 WSES guidelines for the management of severe acute pancreatitis”. World Journal of Emergency Surgery 15 (2020): 3.
  • Tarasconi A. et al. “Perforated and bleeding peptic ulcer: WSES guidelines”. World Journal of Emergency Surgery 15 (2020): 3.
  • Pisano M., et al. “2020 World Society of Emergency Surgery updated guidelines for the diagnosis and treatment of acute calculus cholecystitis”. World Journal of Emergency Surgery 15
    (2020): 61.
  • Hecker A., et al. “Intra-abdominal sepsis: new definitions and current clinical standards”. Langenbeck's Archives of Surgery 404 (2019): 257-271.
  • Gans S.L., et al. “Guideline for the diagnostic pathway in patients with acute abdominal pain”. Digestive Surgery 1 (2015): 23-31.
  • Kuyun LA. “Differential diagnostics of different forms of peritonitis by means of Chemiluminescence” Bulletin of Problems in Biology and Medicine138 (2017): 21-24.
  • Grynchuk FV., et al. “Experimental justification of definition of optical density plasma venous blood for diagnostic of intraabdominal inflammatory processes”. Clinical and Experimental Pathology1 (2011): 38-40.
  • Shurma AI., et al. “Preliminary Evaluation of Possibilities for Determination of Optical Density of Venous Blood Plasma for Diagnosis in Emergency Abdominal Surgery”. Acta Scientific Gastrointestinal Disorders10 (2021): 49-52.
  • Sartelli M., et al. “WSES/GAIS/SIS-E/WSIS/AAST global clinical pathways for patients with intra-abdominal infections”. World Journal of Emergency Surgery1 (2021): 49.
  • Lee M.J., et al. “Dose-dependent mortality and organ injury in a cecal slurry peritonitis model”. Journal of Surgical Science2 (2016): 427-434.
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    Citation

    Citation: Andrii I Shurma and Fedir V Grynchuk. “Investigation of Optical Density of Venous Blood Plasma in Acute Intraabdominal Infection in the Experiment". Acta Scientific Gastrointestinal Disorders 5.6 (2022): 76-79.




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    Acceptance rate35%
    Acceptance to publication20-30 days

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