Andrii I Shurma* and Fedir V Grynchuk
Department of Surgery № 1, Bukovynian State Medical University, Chernivtsi, Ukraine
*Corresponding Author: Andrii I Shurma, Department of Surgery № 1, Bukovynian State Medical University, Chernivtsi, Ukraine.
Received: April 13, 2022; Published: May 27, 2022
Background/Aims: Recognized laboratory criteria for acute intra-abdominal infection, in particular, general blood test, C-reactive protein, are nonspecific and insufficiently informative. We previously reported on the results of venous plasma optical density (ODVBP) measurements in emergency abdominal surgery. But the examined patients differed in the type of disease, sex, etc. Therefore, we conducted experimental studies to substantiate the possibility of using such a criterion.
Methods: 60 white nonlinear mature rats of both sexes, 180 to 200 g weight. To simulate acute intra-abdominal infection, intra-abdominal injection of 10 ml of 10% solution of autofaeces per 100 g of weight was performed. Blood from the jugular vein and pieces of peritoneum for histological examination was taken. The studies were performed before simulation, 6, 12, 24 and 48 h after simulation. ODVBP was examined on an Agilent Cary 100/300 Series UV-Vis spectrophotometer. Healthy animals were monitored for control.
Results: In healthy animals at a wavelength l = 310 nm there is a specific maximum ODVBP, reaching 0.22 units. After simulation of acute intra-abdominal infection and the occurrence of serous peritonitis, there was a statistically significant increase in ODVBP at this wavelength. After the onset of purulent peritonitis, ODVBP also increased statistically significantly. Subsequently, after the spread of inflammation to the deep tissues of the abdominal wall, ODVBP was at a high level and statistically significantly exceeded the control values.
Keywords: Inflammation; Acute Intra-Abdominal Infection; Venous Blood Plasma; Optical Density of Venous Blood Plasma
Citation: Andrii I Shurma and Fedir V Grynchuk. “Investigation of Optical Density of Venous Blood Plasma in Acute Intraabdominal Infection in the Experiment". Acta Scientific Gastrointestinal Disorders 5.6 (2022): 76-79.
Copyright: © 2022 Andrii I Shurma and Fedir V Grynchuk. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.