Camargo CP2, Kubrusly MS1, Lee AD1, Traldi MC3, Maluf FC, Costa AC4, Figueira ERR, Gemperli R2, Carneiro-D’Albuquerque LA1 and Galvão FH1*
1LIM 37, Laboratorio de Transplante e Cirurgia de Figado, Departamento de Gastroenterologia, Hospital das Clinicas HCFMUSP, Faculdade de Medicina, Universidade de Sao Paulo, Sao Paulo, SP, Brazil
2LIM 04, Laboratorio de Microcirurgia e Cirurgia Plastica, Hospital das Clinicas HCFMUSP, Faculdade de Medicina, Universidade de Sao Paulo, Sao Paulo, SP, Brazil
3Resident of Departamento de Cirurgia, Hospital das Clinicas HCFMUSP, Faculdade de Medicina, Universidade de Sao Paulo, Sao Paulo, SP, Brazil
4Departamento de Patologia, Hospital das Clinicas HCFMUSP, Faculdade de Medicina, Universidade de Sao Paulo, Sao Paulo, SP, Brazil
*Corresponding Author: Galvão FH, LIM 37, Laboratorio de Transplante e Cirurgia de Figado, Departamento de Gastroenterologia, Hospital das Clinicas HCFMUSP, Faculdade de Medicina, Universidade de Sao Paulo, Sao Paulo, SP, Brazil.
Received: October 18, 2021; Published: December 09, 2021
Introduction: Enterocutaneous fistula (ECF) is a hazardous postoperative complication in abdominal surgeries that demand costly treatment and can reach a mortality incidence as high as 30%. There is a lack of animal models of ECF to test new strategies treatment. In this study we describe in detail an efficient and simplified model of ECF.
Methods: After general anesthesia, thirteen male Lewis rats were submitted to a midline incision. The cecum was identified and exteriorized through a small incision in the right flank to create the enterocutaneous fistula. The animals were observed twice a week searching for clinical signs of complications (abdominal distention, piloerection, infection, herniation, necrosis or fistula) and body weight assessment. At the 30th post-operative day (POD), the animals were euthanized to perform autopsy, evaluating abdominal cavity and collecting fistula samples for histology (HE).
Results: One animal was sacrificed at the 2nd POD because intestinal prolapse. The remaining 12 animals lost around 10% of the original weight, presented signs of moderate clinical stress but survived untill 30th POD with a steady stercoral fistula and continuous feces output. Histological analysis confirmed the diagnosis of fistula, observing an important lymphomononuclear component in subserous layer and ulceration of squamous epithelium in the fistula border.
Conclusion: This simplified model was effective to create a consistent enterocutaneous fistula with continuous output during 30 days.
Keywords: Intestine; Fistula; Model; Rat
et al. “Zur Methodik der Ileostomie bei der Laborratte”. Z Versuchstierkd 23 (1981): 46-51.Citation: Galvão FH., et al. “Simplified Model of Enterocutaneous Fistula in the Rat". Acta Scientific Gastrointestinal Disorders 5.1 (2022): 06-10.
Copyright: © 2022 Galvão FH., et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.