Camargo CP², Kubrusly MS¹, Lee AD1, Traldi MC³, Maluf FC, Costa AC⁴, Figueira ERR, Gemperli R², Carneiro-D’Albuquerque LA¹ and Galvão FH¹*

¹LIM 37, Laboratorio de Transplante e Cirurgia de Figado, Departamento de Gastroenterologia, Hospital das Clinicas HCFMUSP, Faculdade de Medicina, Universidade de Sao Paulo, Sao Paulo, SP, Brazil
²LIM 04, Laboratorio de Microcirurgia e Cirurgia Plastica, Hospital das Clinicas HCFMUSP, Faculdade de Medicina, Universidade de Sao Paulo, Sao Paulo, SP, Brazil
³Resident of Departamento de Cirurgia, Hospital das Clinicas HCFMUSP, Faculdade de Medicina, Universidade de Sao Paulo, Sao Paulo, SP, Brazil
⁴Departamento de Patologia, Hospital das Clinicas HCFMUSP, Faculdade de Medicina, Universidade de Sao Paulo, Sao Paulo, SP, Brazil

*Corresponding Author: Galvão FH, LIM 37, Laboratorio de Transplante e Cirurgia de Figado, Departamento de Gastroenterologia, Hospital das Clinicas HCFMUSP, Faculdade de Medicina, Universidade de Sao Paulo, Sao Paulo, SP, Brazil.

Received: October 18, 2021; Published: December 09, 2021

Abstract

Introduction: Enterocutaneous fistula (ECF) is a hazardous postoperative complication in abdominal surgeries that demand costly treatment and can reach a mortality incidence as high as 30%. There is a lack of animal models of ECF to test new strategies treatment. In this study we describe in detail an efficient and simplified model of ECF.

Methods: After general anesthesia, thirteen male Lewis rats were submitted to a midline incision. The cecum was identified and exteriorized through a small incision in the right flank to create the enterocutaneous fistula. The animals were observed twice a week searching for clinical signs of complications (abdominal distention, piloerection, infection, herniation, necrosis or fistula) and body weight assessment. At the 30^th post-operative day (POD), the animals were euthanized to perform autopsy, evaluating abdominal cavity and collecting fistula samples for histology (HE).

Results: One animal was sacrificed at the 2^nd POD because intestinal prolapse. The remaining 12 animals lost around 10% of the original weight, presented signs of moderate clinical stress but survived untill 30^th POD with a steady stercoral fistula and continuous feces output. Histological analysis confirmed the diagnosis of fistula, observing an important lymphomononuclear component in subserous layer and ulceration of squamous epithelium in the fistula border.

Conclusion: This simplified model was effective to create a consistent enterocutaneous fistula with continuous output during 30 days.

Keywords: Intestine; Fistula; Model; Rat

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Citation

Citation: Galvão FH., et al. “Simplified Model of Enterocutaneous Fistula in the Rat". Acta Scientific Gastrointestinal Disorders 5.1 (2022): 06-10.

Copyright

Copyright: © 2022 Galvão FH., et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.