Acta Scientific Gastrointestinal Disorders (ASGIS)(ISSN: 2582-1091)

Research Article Volume 5 Issue 1

Simplified Model of Enterocutaneous Fistula in the Rat

Camargo CP2, Kubrusly MS1, Lee AD1, Traldi MC3, Maluf FC, Costa AC4, Figueira ERR, Gemperli R2, Carneiro-D’Albuquerque LA1 and Galvão FH1*

1LIM 37, Laboratorio de Transplante e Cirurgia de Figado, Departamento de Gastroenterologia, Hospital das Clinicas HCFMUSP, Faculdade de Medicina, Universidade de Sao Paulo, Sao Paulo, SP, Brazil
2LIM 04, Laboratorio de Microcirurgia e Cirurgia Plastica, Hospital das Clinicas HCFMUSP, Faculdade de Medicina, Universidade de Sao Paulo, Sao Paulo, SP, Brazil
3Resident of Departamento de Cirurgia, Hospital das Clinicas HCFMUSP, Faculdade de Medicina, Universidade de Sao Paulo, Sao Paulo, SP, Brazil
4Departamento de Patologia, Hospital das Clinicas HCFMUSP, Faculdade de Medicina, Universidade de Sao Paulo, Sao Paulo, SP, Brazil

*Corresponding Author: Galvão FH, LIM 37, Laboratorio de Transplante e Cirurgia de Figado, Departamento de Gastroenterologia, Hospital das Clinicas HCFMUSP, Faculdade de Medicina, Universidade de Sao Paulo, Sao Paulo, SP, Brazil.

Received: October 18, 2021; Published: December 09, 2021

Abstract

Introduction: Enterocutaneous fistula (ECF) is a hazardous postoperative complication in abdominal surgeries that demand costly treatment and can reach a mortality incidence as high as 30%. There is a lack of animal models of ECF to test new strategies treatment. In this study we describe in detail an efficient and simplified model of ECF.

Methods: After general anesthesia, thirteen male Lewis rats were submitted to a midline incision. The cecum was identified and exteriorized through a small incision in the right flank to create the enterocutaneous fistula. The animals were observed twice a week searching for clinical signs of complications (abdominal distention, piloerection, infection, herniation, necrosis or fistula) and body weight assessment. At the 30th post-operative day (POD), the animals were euthanized to perform autopsy, evaluating abdominal cavity and collecting fistula samples for histology (HE).

Results: One animal was sacrificed at the 2nd POD because intestinal prolapse. The remaining 12 animals lost around 10% of the original weight, presented signs of moderate clinical stress but survived untill 30th POD with a steady stercoral fistula and continuous feces output. Histological analysis confirmed the diagnosis of fistula, observing an important lymphomononuclear component in subserous layer and ulceration of squamous epithelium in the fistula border.

Conclusion: This simplified model was effective to create a consistent enterocutaneous fistula with continuous output during 30 days.

Keywords: Intestine; Fistula; Model; Rat

et al. “Zur Methodik der Ileostomie bei der Laborratte”. Z Versuchstierkd 23 (1981): 46-51.

References

  1. Berry S M and Fischer J E. “Classification and pathophysiology of enterocutaneous fistulas”. Surgical Clinics of North America 5 (1996): 1009-1018.
  2. Gribovskaja-Rupp I and Melton GB. “Enterocutaneous Fistula: Proven Strategies and Updates”. Clinics in Colon and Rectal Surgery 2 (2016): 130-137.
  3. Wercka J., et al. “Epidemiology and outcome of patients with postoperative abdominal fistula”. Revista do Colégio Brasileiro de Cirurgiões 2 (2016): 117-123.
  4. Fischer PE., et al. “A ten-year review of enterocutaneous fistulas after laparotomy for trauma”. Journal of Trauma 5 (2009): 924-928.
  5. Hatchimonji JS., et al. “Enterocutaneous fistula after emergency general surgery: Mortality, readmission, and financial burden”. The Journal of Trauma and Acute Care Surgery 1 (2020): 167-172.
  6. Quinn M., et al. “Management of Enterocutaneous Fistula: Outcomes in 276 Patients”. World Journal of Surgery 41 (2017): 2502-2511.
  7. Winder J S and Pauli E M. “Comprehensive management of full-thickness luminal defects: the next frontier of gastrointestinal endoscopy”. World Journal of Gastrointestinal Endoscopy's8 (2015): 758-768.
  8. Avalos-González J., et al. “Reduction of the closure time of postoperative enterocutaneous fistulas with fibrin sealant”. World Journal of Gastroenterology22 (2010): 2793-2800.
  9. Huang JJ., et al. “3D-printed “fistula stent” designed for management of enterocutaneous fistula: An advanced strategy”. World Journal of Gastroenterology41 (2017): 7347-7494.
  10. Lee ADW., et al. “Home parenteral nutrition program and referral of potential candidates for intestinal and multivisceral transplantation in a single Brazilian center”. Transplantation Proceedings 46 (2014): 1839-1841.
  11. Galvão FH., et al. “Experimental animal model of graft-versus-host disease (GVHD) after small-bowel transplantation: characteristics of the model and application to developing treatment strategies”. Transplantation Proceedings 1-2 (1997): 700.
  12. Galvão FH., et al. “Anorectal transplantation”. Techniques in Coloproctology 1 (2009): 55-59.
  13. Galvão FH., et al. “Simplified rat model of intestinal transplantation”. Transplantation 10 (2005): 1522-1523.
  14. Galvão FH., et al. “Modified multivisceral transplantation in the rat”. Transplantation 2 (2013): e3-4.
  15. Galvão FH., et al. “Allogeneic anorectal transplantation in rats: technical considerations and preliminary results”. Scientific Report 6 (2016): 30894.
  16. Bültmann O., et al. “Creation of an enterocutaneous fistula”. Research in Experimental Medicine (Berl)4 (1998): 215-228.
  17. Wittig G., et al. “Der Einfluß der Ingesta auf die Kanzerisierung des Rattendarmes durch Dimethylhydrazin”. Arch Geschwulstforsch 37 (1971): 105-115.
  18. Kohn M. “Satiation of hunger from stomach versus mouth feeding”. Journal of Comparative and Physiological Psychology 44 (1951): 412-422.
  19. Bleyl DWR., et al. “Zur Methodik der Ileostomie bei der Laborratte”. Z Versuchstierkd 23 (1981): 46-51.

Citation

Citation: Galvão FH., et al. “Simplified Model of Enterocutaneous Fistula in the Rat". Acta Scientific Gastrointestinal Disorders 5.1 (2022): 06-10.

Copyright

Copyright: © 2022 Galvão FH., et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.




Metrics

Acceptance rate35%
Acceptance to publication20-30 days

Indexed In




News and Events


  • Certification for Review
    Acta Scientific certifies the Editors/reviewers for their review done towards the assigned articles of the respective journals.
  • Submission Timeline for Upcoming Issue
    The last date for submission of articles for regular Issues is July 10, 2022.
  • Publication Certificate
    Authors will be issued a "Publication Certificate" as a mark of appreciation for publishing their work.
  • Best Article of the Issue
    The Editors will elect one Best Article after each issue release. The authors of this article will be provided with a certificate of “Best Article of the Issue”.
  • Welcoming Article Submission
    Acta Scientific delightfully welcomes active researchers for submission of articles towards the upcoming issue of respective journals.
  • Contact US