Abstract

Morphological peculiarities of liver after antigen influence on the mother-placenta-fetus system is not well studied.

Materials and methods: The study included the livers of 144 white laboratory rats from 1 to 90 days of life. All animals were conditionally divided into 3 groups: Group I - intact animals, which were born from healthy rats without any antigen administration during pregnancy; group II - control animals, which were exposed to antenatal intrafetal administration of 0.05 ml saline solution on the 18^th day of prenatal development; group III - experimental animals, which were exposed to antenatal antigen administration at 18^th day of prenatal development. The absolute and relative mass of the liver was calculated. For light microscopy, sections were stained with hematoxylin and eosin. Detection of β-D-galactose carbohydrate residues was performed using a Lectin from Ricinus communis Agglutinin (RCA) by standard methods, using standard set of reagents "LectinTest" (Lviv). Quantitative and qualitative morphological characteristics of the liver were studied. Analysis of the obtained results was conducted by means of statistical methods with the use of computer license program “Statistica for Windows 13” (StatSoft Inc., № JPZ804I382130ARCN10-J).

Results: In experimental animals after antenatal administration of staphylococcal toxoid, the relative area occupied by hepatic plates (as in the control) tends to increase gradually during the first two weeks of life. In animals, which were exposed to antenatal Staphylococcal purified liquid toxoid administration, the number of lymphocytes in the central zone of the liver lobule is lower than in the intact and control groups on the first day is 1.04 ± 0.35 cells on unit area. (2.26 ± 0.52 cells on unit area in control). The maximum decrease in the number of lymphocytes in the central zone in experimental rats in contrast to the control occurs on the 30^th day of life together with a simultaneous decrease in the number of Kupffer macrophages and is 0.70 ± 0.35 cells on unit area - in the experiment (2.61 ± 0.69 cells on unit area in the control group). A decrease in the number of lymphocytes is observed also in the peripheral zone of the lobule compared with the control from the third to the 30^th day of life, but such changes have no statistical significance. It was found that RCA+ receptors are expressed on dendritic cells in the liver, the number of RCA+ dendritic cells changes in waves during the first three months after birth.

Conclusions: The relative mass of the liver in experimental group rats significantly exceeds the control at the seventh day of life (3.06 ± 0.05% in the experimental and 2.91 ± 0.02% in the control group). In experimental rats the number of dendritic cells decreases during the first two weeks of life, the minimum number is observed on the 3^rd day. In the peripheral zone of the lobule, a decrease in the number of dendritic cells is observed on the 1^st and the 21^st day of postnatal life in comparison with control.

Keywords: Liver; Intranatal Antigen Influence; Dendritic Cells

References

1. Dovgal GV. “Morphological changes in the development of rat liver after the lead acetate treatment and under the correction in the prenatal period”. Український морфологічний альманах 12.1 (2014): 42-44.
2. Grynivetska NV and Hryhorieva OA. “Dynamics of distribution WGA+ receptors in pancreas in norm and after intranatal action of antigens”. Reports of Vinnytsia National Medical University 23.31 (2019): 94-97.
3. Grynivetska NV. “Dynamics of pancreas mass indexes in early postnatal period after intranatal antigen injection [in Ukraine]”. Actual questions of pharmacy and medical science and practice 14 (2011): 14.
4. Hryhorieva O., et al. “Intranatal antigen injection as an experimental model of undifferentiated connective tissue syndrome”. Abstracts of V International Scientific and practical Conference “Study of modern problems of civilization”, Oslo, Norway 19-23 October, (2020): 206-208.
5. Hryhorieva OA and Grynivetska NV. “Morphological features of alteration of pancreaic stroma in rats with experimental undifferentiated dysplasy of connective tissue [in Ukraine]”. Clinical Anatomy and Operative Surgery31 (2019): 46-51.
6. Hryhorieva OA., et al. “Peculiarities of synoviocytes and chondrocytes proliferative activity in rats with experimental model of undifferentiated dysplasia of connective tissue”. World of Medicine and Biology 76 (2021): 198-202.
7. Hryhorieva O A., et al. “Intrafetal injection of antigens as an experimental model of undifferentiated dysplasia of connective tissue”. Medico-biological aspects and multidisciplinary integration in the concept of human health : proceedings of Ukr. Conf. with int. Participation (Ternopil, 9-11 April 2020.) in III parts/Ternopil. Nat.Med.Univ named after I Horbachevsky MPH of Ukraine. - Ternopil : TNMU, 2020. -Part I (2020): 37-40.
8. Slobodyan OM., et al. “Prenatal development of hepatic veins”. Clinical Anatomy and Operative Surgery2 (2015): 38-41.
9. Voloshyn MA., et al. “Visceromegalia of newborns: morphological aspects”. Kharkiv: Kharkiv State Medical University (2004): 123.
10. Voloshyn MA., et al. “Intranatal antigen injection as a model of visceromegalia sympomocomlex”. Tavria Medico-Biological Reports3/4 (2006): 41-44.
11. Voloshin NA and Hrihorieva OA. “Experimental model of undifferentiated connective tissue dysplasya syndrome”. Pathologia1 (2009): 39-42.
12. Voloshyn MA and Hryhorieva OA. “Experimental model of undifferentiated dysplasy of connective tissue by impaired antigen homeostasis in the system of mother-placenta-foetus”. Pathologia2 (2011): 39-42.
13. Znamenska T K., et al. “Strategichni napryamki rekonstruktcii systemi ohoroni zdorovya novonarodjenich ta ditey Ukraini [Strategic directions of reconstruction of the system of health guard of new-born and children of Ukraine]”. Social'na Pediatriya Ta Reabilitologiya -Social Paediatrics and Rehabilitology 1-2.13-14 (2018): 7-14.

Citation

Citation: Pivtorak VI., et al. “Morphological Features of Liver Reactivity After Intranatal Antigen Influence”. Acta Scientific Gastrointestinal Disorders 4.11 (2021): 72-83.

Copyright

Copyright: © 2021 Pivtorak VI., et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.