Acta Scientific Gastrointestinal Disorders (ASGIS)(ISSN: 2582-1091)

Research Article Volume 4 Issue 6

Detection of Human Intestinal Parasites in Commonly Consumed Fresh Leafy Vegetables in Al-Baha Region, Saudi Arabia

Eman M Hussein1,4*, Nawal M Osman2, Sarah A Al-Harbi3, Ahlam S Al Abbad3, Noof A Alghamdi3, Raghad A Alghamdi3, Lama M Alkhediwi3, Safa S Al Zahrani3, Yakin H Alhaddad3 and Ala A Atta5

1Microbiology Department, Faculty of Medicine, Al-Baha University, Al-Baha, KSA
2Family Medicine and Community Department, Faculty of Medicine, Al-Baha University, Al-Baha, KSA
3Medical Students, Faculty of Medicine, Al Baha University, Al Baha, KSA
4Medical Parasitology, Faculty of Medicine, Suez Canal University, Ismailia, Egypt
5Microbiology Department, Faculty of Dentists, Al-Baha University, Al-Baha, KSA

*Corresponding Author: Eman M Hussein, Microbiology Department, Faculty of Medicine, Al-Baha University, Al-Baha, KSA and Medical Parasitology, Faculty of Medicine, Suez Canal University, Ismailia, Egypt.

Received: March 23, 2021; Published: May 17, 2021

Abstract

Introduction: Ingestion of contaminated fresh leafy vegetables had an important role in the transmission of several intestinal parasitic diseases.

Objectives: This study aimed to identify human intestinal parasites in commonly consumed fresh leafy vegetables in Al-Baha Region, Saudi Arabia.

Methodology: In the current study, 400 leafy vegetable samples collected randomly from the supermarkets and open-aired markets at different times of the year in Al-Baha city were examined to detect the frequency of contamination with human intestinal parasites. Sediments and supernatants of concentrated washing solution of vegetables were examined after stained by iodine and modified Ziehl-Neelsen.

Results: The leafy vegetables contaminated with intestinal parasites were 41% (164/400). The most contaminated leafy vegetables were spinach, red radish, watercress and lettuce with 58.3%, 57.5%, 55% and 50%, respectively, while the less contaminated were mint and cabbage with 16.6% per each. Green onions, parsley, dill, celery, leek and coriander were contaminated with 40%, 37.5%, 33.3%, 31.2%, 25% and 21.9%, respectively. Contaminated leafy vegetables collected from open-air markets was 41% compared to 33% of that were collected from supermarkets. These results were statistically significant. About 122 leafy vegetables out of 164 (74.3%) were contaminated with helminths. Taeniid eggs and Strongyloides larvae were the most common with 18.3% (30 leafy vegetables per each). About 141 leafy vegetables out of 164 (85.9%) were contaminated with pathogenic human intestinal protozoa. Cryptosporidium oocysts and Giardia lamblia cysts were the most commonly found with 48 (29.3%) and 35 (21.3%), respectively. The highest prevalence was in spring (45.5%) and the lowest was in summer (33.3%). These results were statistically significant.

Conclusion: Control measures should include treatment of irrigation water, municipal wastewater, treatment of infected persons and mass education of the persons in contact with fresh leafy vegetables to avoid a greater health risk from handling and consuming these vegetables.

Keywords: Diabetes Mellitus; Metabolism; Visceral Fat; Insulin; Cholesterol

References

  1. Fleury MD., et al. “A descriptive analysis of hospitalization due to acute gastrointestinal illness in Canada, 1995 -2004”. Canadian Journal of Public Health 99 (2008): 489-493.
  2. WHO “First Formal Meeting of the Foodborne Disease Burden Epidemiology Reference Group (FERG)” 26-28 (2007).
  3. Helms M., et al. “Short and long term mortality associated with foodborne bacterial gastrointestinal infections: registry-based study”. British Medical Journal 326 (2003): 357.
  4. Jay LS., et al. “A video study of Australian domestic food-handling practices”. Journal of Food Protection 62 (2016): 921-928.
  5. Finley R., et al. “High-risk food consumption and food safety practices in a Canadian community”. Journal of Food Protection 72 (2009): 2575-2586.
  6. Redmond EC and Griffith CJ. “Consumer food handling in the home: a review of food safety studies”. Journal of Food Protection 66 (2003): 130-161.
  7. Redmond EC., et al. “Microbiological and observational analysis of cross-contamination risks during domestic food preparation”. British Food Journal 106 (2004): 581-597.
  8. Kang GM., et al. “Prevalence of intestinal parasites in rural Southern Indians”. Tropical Medicine and International Health 3 (1998): 70.
  9. WHO: Schistosomiasis and soil-transmitted helminth infections. Fifty-Fourth World Health Assembly. Volume WHA54.19 (2001).
  10. Vuong TA., et al. “Fecal and protozoan parasite contamination of water spinach (Ipomoea aquatic) cultivated in urban wastewater in Phnom Penh, Cambodia”. Tropical Medicine and International Health 12 (2007): 73-81.
  11. De Oliveira CA and PM Germano. “Presence of intestinal parasites in vegetables sold in the metropolitan area of Sao Paulo-SP, Brazil II-Research on intestinal protozoans”. Revista de Saúde Pública 26 (1992): 332-335.
  12. Dawson D. “Foodborne protozoan parasites”. International Journal of Food Microbiology 2 (2005): 207-227.
  13. Duedu O., et al. “A comparative study of the prevalence of human parasites found in fresh vegetables sold in supermarkets and open-aired market in Accra, Ghana”. BMC Research Notes 7 (2014): 836.
  14. Al- Mohamed M., et al. “Parasitic contamination of fresh vegetables sold at central market in Khartoum state, Sudan”. Annals of Clinical Microbiology and Antimicrobials 15 (2016): 17.
  15. Cable J., et al. “Global change, parasite transmission, and disease control: lessons from ecology”. Philosophical Transactions of the Royal Society B: Biological Sciences 1719 (2017): 20160088.
  16. Hoberg EP and Brooks DR. “Evolution in action: climate change, biodiversity dynamics and emerging infectious disease”. Philosophical Transactions of the Royal Society B: Biological Sciences 1665 (2015): 20130553.
  17. Albinali AM. “The prevalence of parasites in commonly used leafy vegetables in southwestern, Saudi Arabia”. Saudi Medical Journal 27 (2006): 613-616.
  18. Al-Megrin W. “Prevalence of intestinal parasites in leafy vegetables in Riyadh, Saudi Arabia”. International Journal of Zoological Research 3 (2010): 190-195.
  19. Climate data of Saudi Arabia Jeddah Regional Climate Center. “Archived from the original on 2012-05-12”. (2016).
  20. Alqumber MA. “Parasitic Contamination in Raw Vegetables in Cities of the Sarawat Mountain Range of Saudi Arabia”. Life Science Journal 11 (2014): 9.
  21. Abougrains AK., et al. “Parasitological contamination in salad vegetables in Tripoli-Libya”. Food Control5 (2010): 760-762.
  22. Garcia L. “Macroscopic and microscopic examination of fecal specimen”. In diagnostic medical parasitology, 5th ed. ASM Press, Washington, DC (2007): 732-830.
  23. Gabber R and Shaker A. “Prevalence of some human enteroparasites in commonly consumed raw vegetables in Tabuk Saudi Arabia”. Journal of Food Protection4 (2014): 655-658.
  24. Ammar A and Omar H. “The prevalence of leafy vegetable-borne parasites in Al-Qassim region Saudi Arabia”. Journal of Agriculture and Veterinary Sciences. Al Qassim University 6 (2013): 29-40.
  25. Abougrain AK., et al. “Parasitological contamination in salad vegetables in Tripoli-Libya”. Food Control5 (2010): 5760-5762.
  26. Hadi AM. “Isolation and identification of intestinal parasites from vegetables from different markets of Iraq”. Bulletin of the Iraq Natural History Museum4 (2011): 17-25.
  27. Nyarango RM., et al. “The risk of pathogenic intestinal parasite infections in Kisii Municipality, Kenya”. BMC Public Health1 (2008): 237.
  28. Said DE. “Detection of parasites in commonly consumed raw vegetables”. Alexandria Journal of Medicine 48 (2012): 345-352.
  29. Eraky MA., et al. “Parasitic Contamination of Commonly Consumed Fresh Leafy Vegetables in Benha, Egypt”. Journal of Parasitology Research 14 (2014).
  30. Alsayeqh AF. “Foodborne disease risk factor among women in Riyadh, Saudi Arabia”. Food Control. 50 (2015): 85-91.
  31. Omar Amer., et al. “Strongyloides stercoralis in leafy vegetables, humans, and cats as a possible source for zoonotic disease in Hail, Saudi Arabia”. International Journal of Medicine in Developing Countries6 (2019): 553-556.
  32. Mejia R., et al. “Screening prevention, and treatment of hyperinfection syndrome and disseminated infections caused by Strongyloides stercoralis”. Current Opinion in Infectious Diseases - LWW Journals 25 (2012): 458-463.
  33. Macarisin D., et al. “Leaf Stomata Harboring Cryptosporidium parvum Oocysts: a Potential Threat to Food Safety”. Applied and Environmental Microbiology2 (2010): 555-559.
  34. Bidya Prasad., et al. “A method for incorporating a time-dose-response model into a Giardia lamblia outbreak”. Journal of Water and Health 15.4 (2017): 490-504.
  35. Messner M and Berger P. “Cryptosporidium infection risk result of new dose-response modeling”. Risk Analysis 36 (2016): 1969-1982.
  36. Uga S., et al. “Parasite egg contamination of vegetables from a suburban market in Hanoi, Vietnam”. Nepal Medical College Journal2 (2009): 75-78.
  37. Adanir R and. Tasci F. “Prevalence of helminth eggs in raw vegetables consumed in Burdur, Turkey”. Food Control 2 (2013): 482-484.
  38. Eslami A., et al. “Study on the prevalence seasonal incidence and economic importance of parasitic infections of small ruminants in the province of Semnan”. Pajouhesh Sazandegi 58 (2003): 55-58.

Citation

Citation: Eman M Hussein., et al. “Detection of Human Intestinal Parasites in Commonly Consumed Fresh Leafy Vegetables in Al-Baha Region, Saudi Arabia”. Acta Scientific Gastrointestinal Disorders 4.6 (2021): 39-47.

Copyright

Copyright: © 2021 Eman M Hussein., et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.




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